Gene ea ho Hanyetsa Carbapenem (KPC/NDM/OXA 48/OXA 23/VIM/IMP)
Lebitso la sehlahisoa
Kiti ea ho lemoha ea HWTS-OT045 Carbapenem Resistance Gene (KPC/NDM/OXA 48/OXA 23/VIM/IMP) (Fluorescence PCR)
Thuto ea mafu a seoa
Lithibela-mafu tsa Carbapenem ke lithibela-mafu tse sa tloaelehang tsa β-lactam tse nang le mefuta e mengata ea lithibela-mafu le tšebetso e matla ea lithibela-mafu. Ka lebaka la botsitso ba eona ho β-lactamase le chefo e tlase, e fetohile e 'ngoe ea lithibela-mafu tsa bohlokoa ka ho fetisisa bakeng sa kalafo ea tšoaetso e matla ea baktheria. Li-Carbapenems li tsitsitse haholo ho li-β-lactamase tse atolositsoeng ...
Mokero
| Motsoako oa PCR 1 | Motsoako oa PCR 2 | |
| FAM | IMP | VIM |
| VIC/HEX | Taolo ea Ka Hare | Taolo ea Ka Hare |
| CY5 | NDM | KPC |
| ROX | OXA48
| OXA23 |
Li-parameter tsa Tekheniki
| Polokelo | ≤-18℃ |
| Nako ea ho phela | Likhoeli tse 12 |
| Mofuta oa Sampole | Sekhohlela, likolone tse hloekileng, sesepa sa rectal |
| Ct | ≤36 |
| CV | ≤5.0% |
| LoD | 103CFU/mL |
| Ho hlaka | a) Sete ena e lemoha litšupiso tse mpe tsa k'hamphani tse tloaelehileng, 'me liphetho li fihlela litlhoko tsa litšupiso tse tsamaellanang. b) Liphetho tsa teko ea ho kopanya lisele li bontša hore kit ena ha e na karabelo e tšoanang le likokoana-hloko tse ling tsa phefumoloho, tse kang Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, Streptococcus pneumoniae, Neisseria meningitidis, Staphylococcus aureus, Klebsiella oxytoca, Haemophilus influenzae, Acinetobacter junii, Acinetobacter haemolyticus, Legionella pneumophila, Escherichia coli, Pseudomonas fluorescens, Candida albicans, Chlamydia pneumoniae, Respiratory adenovirus, Enterococcus, kapa lisampole tse nang le liphatsa tse ling tsa lefutso tse hanyetsanang le meriana CTX, mecA, SME, SHV, TEM, jj. c) Thibelo ea tšitiso: Mucin, Minocycline, Gentamicin, Clindamycin, Imipenem, Cefoperazone, Meropenem, Ciprofloxacin Hydrochloride, Levofloxacin, Clavulanic acid, Roxithromycin li khethiloe bakeng sa teko ea tšitiso, 'me liphetho li bontša hore lintho tse tšitiso tse boletsoeng ka holimo ha li na karabelo e tšitiso ho fumanoeng ha liphatsa tsa lefutso tsa ho hanyetsa carbapenem KPC, NDM, OXA48, OXA23, VIM, le IMP. |
| Lisebelisoa tse Sebetsang | Mekhoa ea Li-biosystem tse Sebelisitsoeng 7500 Mekhoa ea PCR ea Nako ea Sebele Mekhoa ea PCR ea QuantStudio®5 ea Nako ea 'Nete Mekhoa ea PCR ea Nako ea 'Nete ea SLAN-96P (Hongshi Medical Technology Co., Ltd.) Sistimi ea PCR ea LightCycler®480 ea Nako ea 'Nete Sistimi ea ho lemoha PCR ea LineGene 9600 Plus ea Nako ea 'Nete (FQD-96A，HangzhouTheknoloji ea Bioer) Sesebelisi sa Thermal sa MA-6000 sa Nako ea Sebele sa Bongata ba Thermal (Suzhou Molarray Co., Ltd.) Sistimi ea PCR ea BioRad CFX96 ea Nako ea 'Nete Sistimi ea PCR ea BioRad CFX Opus 96 ea Nako ea 'Nete |
Phallo ea Mosebetsi
Khetho ea 1.
Reagent e kgothaletswang ya ho ntsha: Kit ya DNA/RNA e Akaretsang ya Macro le Micro-Test (HWTS-301)9-50, HWTS-3019-32, HWTS-3019-48, HWTS-3019-96) (e ka sebelisoang le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) ke Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.. Kenya 200μL ea letsoai le tloaelehileng ho thallus precipitate. Mehato e latelang e lokela ho latela litaelo tsa ho ntša, 'me bophahamo bo khothaletsoang ba elution ke100μL.
Khetho ea 2.
Reagent e kgothalletswang ya ho ntsha: Nucleic Acid Extraction kapa Purification Reagent (YDP302) ke Tiangen Biotech (Beijing) Co., Ltd. Ho ntsha ho lokela ho qalwa ho ya ka mohato wa 2 wa taelo ya tshebediso (eketsa 200μL ya buffer GA ho thallus precipitate, mme o sisinye ho fihlela thallus e emisitsoe ka botlalo). Sebedisa metsi a se nang RNase/DNase bakeng sa elution, mme bophahamo ba elution bo kgothaletswang ke 100μL.
Khetho ea 3.
Reagent e kgothalletswang ho ntsha: Reagent ya Macro & Micro-Test Sample Release. Sampole ya sekhohlela e hloka ho hlatsuwa ka ho eketsa 1mL ya saline e tlwaelehileng ho thallus precipitate e boletsweng ka hodimo, e kentswe ka centrifuge ka 13000r/min metsotso e 5, mme supernatant e lahlwe (boloka 10-20µL ya supernatant). Bakeng sa colony e hlwekileng le rectal swab, eketsa 50μL ya reagent ya sampole ya ho ntsha ka ho toba ho thallus precipitate e boletsweng ka hodimo, mme mehato e latelang e lokela ho ntshuwa ho ya ka taelo ya tshebediso.
