Carbapenem Resistance Gene (KPC/NDM/OXA 48/OXA 23/VIM/IMP)
Lebitso la sehlahisoa
HWTS-OT045 Carbapenem Resistance Gene (KPC/NDM/OXA 48/OXA 23/VIM/IMP) Kit (Fluorescence PCR)
Epidemiology
Lithibela-mafu tsa Carbapenem ke lithibela-mafu tse sa tloaelehang tsa β-lactam tse nang le li-antibacterial spectrum tse pharaletseng ka ho fetisisa le ts'ebetso e matla ea antibacterial. Ka lebaka la botsitso ba eona ho β-lactamase le chefo e tlase, e fetohile e 'ngoe ea litlhare tsa bohlokoa tsa antibacteria bakeng sa kalafo ea mafu a matla a baktheria. Carbapenems e tsitsitse haholo ho plasmid-mediated extended-spectrum β-lactamases (ESBLs), chromosomes, le plasmid-mediated cephalosporinases (AmpC enzymes).
Channel
| PCR-Mix 1 | PCR-Mix 2 | |
| FAM | IMP | VIM |
| VIC/HEX | Taolo ea ka hare | Taolo ea ka hare |
| CY5 | NDM | KPC |
| ROX | OXA48
| OXA23 |
Tekheniki Parameters
| Polokelo | ≤-18℃ |
| Shelf-bophelo | Likhoeli tse 12 |
| Mofuta oa Mohlala | Sekhohlela, likolone tse hloekileng, swab ea rectal |
| Ct | ≤36 |
| CV | ≤5.0% |
| LoD | 103CFU/mL |
| Tse khethehileng | a) Kit e lemoha litšupiso tse mpe tsa k'hamphani tse emeng, 'me liphetho li fihlela litlhoko tsa litšupiso tse tsamaellanang. b) Liphetho tsa tlhahlobo ea cross-reactivity li bonts'a hore kit ena ha e na karabelo ea sefapano le likokoana-hloko tse ling tsa phefumoloho, joalo ka Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, Streptococcus pneumoniae, Neisseria meningitidis, Staphylococcus aureus, Klexytocasiella oreus, Klexytocasiella Acinetobacter junii, Acinetobacter haemolyticus, Legionella pneumophila, Escherichia coli, Pseudomonas fluorescens, Candida albicans, Chlamydia pneumoniae, Respiratory adenovirus, Enterococcus, kapa disampole tse nang le mefuta e meng e hanyetsanang le lithethefatsi CTX, TME, mecSHA jj. c) Ho thibela ho kena-kenana: Mucin, Minocycline, Gentamicin, Clindamycin, Imipenem, Cefoperazone, Meropenem, Ciprofloxacin Hydrochloride, Levofloxacin, Clavulanic acid, Roxithromycin e khethiloe bakeng sa tlhahlobo ea ho kena-kenana, 'me liphello li bontša hore lintho tse sitisang tse boletsoeng ka holimo ha li na ho fumanoa ha genesba carpen, KDM, k'hanyello ea lik'hemik'hale tsa KDM. OXA48, OXA23, VIM, le IMP. |
| Lisebelisoa tse sebetsang | Applied Biosystems 7500 Real-Time PCR Systems QuantStudio®5 Nako ea 'Nete ea PCR Systems SLAN-96P ea Nako ea Sebele PCR Systems (Hongshi Medical Technology Co., Ltd.) Sesebelisoa sa PCR sa LightCycler®480 Real-Time LineGene 9600 Plus Real-Nako PCR Detection System (FQD-96A,HangzhouTheknoloji ea bioer) MA-6000 Nako ea 'Nete ea Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) Sistimi ea PCR ea Nako ea 'Nete ea BioRad CFX96 BioRad CFX Opus 96 Real-Nako PCR System |
Phallo ea Mosebetsi
Khetho ea 1.
Sehlahisoa se khothalelitsoeng: Macro & Micro-Test General DNA/RNA Kit (HWTS-3019-50, HWTS-3019-32, HWTS-3019-48, HWTS-3019-96) (e ka sebelisoang ka Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) ka Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.. Eketsa 200μL ea saline e tloaelehileng ho pula ea thallus. Mehato e latelang e lokela ho latela litaelo tsa ho ntšoa, 'me molumo o khothaletsoang oa elution ke100μL
Khetho ea 2.
Sehlahisoa sa ho ntša se khothalelitsoeng: Nucleic Acid Extraction kapa Purification Reagent (YDP302) ke Tiangen Biotech (Beijing) Co., Ltd. Mokotla o lokela ho qalisoa ho latela mohato oa 2 oa taelo ea tšebeliso (eketsa 200μL ea buffer GA ho pula ea thallus, 'me u sisinyehe ho fihlela thallus e tima ka ho feletseng). Sebelisa metsi a mahala a RNase/DNase bakeng sa ho hlakisoa, 'me bophahamo ba modumo o khothaletsoang ke 100μL.
Khetho ea 3.
Sehlahisoa se khothalelitsoeng sa ho ntša: Macro & Micro-Test Sample Release Reagent. Mohlala oa sekhohlela o hloka ho hlatsuoa ka ho eketsa 1mL ea letsoai le tloaelehileng ho thallus precipitate e boletsoeng ka holimo, e centrifuged ho 13000r/min metsotso e 5, 'me supernatant ea lahloa (boloka 10-20µL ea supernatant). Bakeng sa kolone e hloekileng le swab ea rectal, eketsa 50μL ea reagent ea ho lokolla sampole ka kotloloho ho thallus precipitate e boletsoeng ka holimo, 'me mehato e latelang e lokela ho ntšoa ho latela taelo ea tšebeliso.
