Carbapenem Resistance Gene (KPC/NDM/OXA 48/OXA 23/VIM/IMP)

Tlhaloso e Khutšoanyane:

Setsi sena se sebelisetsoa ho lemoha boleng ba liphatsa tsa lefutso tsa carbapenem ho lisampole tsa sekhohlela sa motho, lisampole tsa rectal swab kapa likolone tse hloekileng, ho akarelletsa le KPC (Klebsiella pneumonia carbapenemase), NDM (New Delhi metallo-β-lactamase 1), OXA48 (oxacillinase 48), OXA23 (oxacillinase 23), VIM (Verona Imipenemase), le IMP (Imipenemase).


Lintlha tsa Sehlahisoa

Li-tag tsa Sehlahisoa

Lebitso la sehlahisoa

HWTS-OT045 Carbapenem Resistance Gene (KPC/NDM/OXA 48/OXA 23/VIM/IMP) Kit (Fluorescence PCR)

Epidemiology

Lithibela-mafu tsa Carbapenem ke lithibela-mafu tse sa tloaelehang tsa β-lactam tse nang le li-antibacterial spectrum tse pharaletseng ka ho fetisisa le ts'ebetso e matla ea antibacterial.Ka lebaka la botsitso ba eona ho β-lactamase le chefo e tlase, e fetohile e 'ngoe ea litlhare tsa bohlokoa tsa antibacteria bakeng sa kalafo ea mafu a matla a baktheria.Carbapenems e tsitsitse haholo ho plasmid-mediated extended-spectrum β-lactamases (ESBLs), chromosomes, le plasmid-mediated cephalosporinases (AmpC enzymes).

Channel

  PCR-Mix 1 PCR-Mix 2
FAM IMP VIM
VIC/HEX Taolo ea ka hare Taolo ea ka hare
CY5 NDM KPC
ROX

OXA48

OXA23

Tekheniki Parameters

Polokelo

≤-18℃

Shelf-bophelo Likhoeli tse 12
Mofuta oa Mohlala Sekhohlela, likolone tse hloekileng, swab ea rectal
Ct ≤36
CV ≤5.0%
LoD 103CFU/mL
Tse khethehileng a) Kit e lemoha litšupiso tse mpe tsa k'hamphani tse emeng, 'me liphetho li fihlela litlhoko tsa litšupiso tse tsamaellanang.

b) Liphetho tsa tlhahlobo ea cross-reactivity li bonts'a hore kit ena ha e na karabelo ea sefapano le likokoana-hloko tse ling tsa phefumoloho, joalo ka Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, Streptococcus pneumoniae, Neisseria meningitidis, Staphylococcus aureus, Klexytobacinella oreus, Klexytobacinella, Klexytobacinella, Klexytobacinella oreus junii, Acinetobacter haemolyticus, Legionella pneumophila, Escherichia coli, Pseudomonas fluorescens, Candida albicans, Chlamydia pneumoniae, Respiratory adenovirus, Enterococcus, kapa lisampole tse nang le mefuta e meng e hanyetsanang le lithethefatsi CTX, mecVA, TEM, joalo-joalo.

c) Khahlanong le tšitiso: Mucin, Minocycline, Gentamicin, Clindamycin, Imipenem, Cefoperazone, Meropenem, Ciprofloxacin Hydrochloride, Levofloxacin, Clavulanic acid, Roxithromycin li khethiloe bakeng sa tlhahlobo ea tšitiso, 'me liphetho li bontša hore lintho tse sitisang tse boletsoeng ka holimo ha li na ho kena-kenana. ho lemoha liphatsa tsa lefutso tsa carbapenem KPC, NDM, OXA48, OXA23, VIM, le IMP.

Lisebelisoa tse sebetsang Applied Biosystems 7500 Real-Time PCR Systems

QuantStudio®5 Nako ea 'Nete ea PCR Systems

SLAN-96P ea Nako ea Sebele PCR Systems (Hongshi Medical Technology Co., Ltd.)

Sesebelisoa sa PCR sa LightCycler®480 Real-Time

LineGene 9600 Plus Real-Nako PCR Detection System (FQD-96A,HangzhouTheknoloji ea bioer)

MA-6000 Nako ea 'Nete ea Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.)

Sistimi ea PCR ea Nako ea 'Nete ea BioRad CFX96

BioRad CFX Opus 96 Real-Nako PCR System

Phallo ea Mosebetsi

Khetho ea 1.

Sehlahisoa se khothalelitsoeng: Macro & Micro-Test General DNA/RNA Kit (HWTS-3019-50, HWTS-3019-32, HWTS-3019-48, HWTS-3019-96) (e ka sebelisoang le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) ka Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Eketsa 200μL ea saline e tloaelehileng ho pula ea thallus.Mehato e latelang e lokela ho latela litaelo tsa ho ntšoa, 'me molumo o khothaletsoang oa elution ke100μL

Khetho ea 2.

Sehlahisoa se khothalelitsoeng sa ho ntšoa: Nucleic Acid Extraction kapa Purification Reagent (YDP302) ke Tiangen Biotech (Beijing) Co., Ltd. Mokotla o lokela ho qalisoa ho latela mohato oa 2 oa taelo ea tšebeliso (eketsa 200μL ea buffer GA ho pula ea thallus. , 'me u sisinyehe ho fihlela thallus e emisoa ka ho feletseng).Sebelisa metsi a mahala a RNase/DNase bakeng sa ho hlakisoa, 'me bophahamo ba modumo o khothaletsoang ke 100μL.

Khetho ea 3.

Sehlahisoa se khothalelitsoeng sa ho ntša: Macro & Micro-Test Sample Release Reagent.Mohlala oa sekhohlela o hloka ho hlatsuoa ka ho kenya 1mL ea letsoai le tloaelehileng ho thallus precipitate e boletsoeng ka holimo, e centrifuged ho 13000r/min metsotso e 5, 'me supernatant ea lahloa (boloka 10-20µL ea supernatant).Bakeng sa kolone e hloekileng le swab ea rectal, eketsa 50μL ea reagent ea ho lokolla sampole ka kotloloho ho thallus precipitate e boletsoeng ka holimo, 'me mehato e latelang e lokela ho ntšoa ho latela taelo ea tšebeliso.


  • E fetileng:
  • E 'ngoe:

  • Ngola molaetsa wa hao mona mme o re romele wona