CarbapeneM Harana (KPC / NDM / Oxa 23 / Oxa 23 / Vim / IND)
Lebitso la Product
Hwts-ot045 carbapenem harane (kpc / ndm
Epidphiology
Lithibelo tsa lithibela-mafu ke lithibela-mafu tsa carbapene. Lithibelo tsa lithibela-mafu. Ka lebaka la botsitso ba eona ho β-lactama le chefo e tlase, e se e le e 'ngoe ea lithethefatsi tse bohlokoa ka ho fetisisa bakeng sa kalafo e matla ea baktheria e matla ea baktheria bakeng sa kalafo e matla ea baktheria e matla bakeng sa kalafo e matla ea baktheria e matla. Carbapenems e tsitsitse haholo ho li-spicrum tse sephara tse koahetsoeng ka har'a plasmid
Channel
| Remix 1 | PCR-Remix 2 | |
| FMAM | IG | Vim |
| Video / Hex | Taolo e ka hare | Taolo e ka hare |
| Cy5 | Ndm | Kpc |
| Rox | Oxa48
| Oxa23 |
Litekanyetso tsa tekheniki
| Polokelo | ≤-18 ℃ |
| Shell-Bophelo | Likhoeli tse 12 |
| Mofuta oa SIMON | Sputum, likolone tse hloekileng, liponko |
| Ct | ≤36 |
| CV | ≤ 40.0% |
| Lod | 103Cfu / ml |
| TLHOKOMELISO | a) Sehlopha se ana litšupiso tse mpe tsa k'hamphani, 'me litholoana li fihlela litlhoko tsa litšupiso tse tsamaellanang. b) Liphetho tsa teko e bakang sefapano li bonts'a hore na boholo bona ha bo na karabelo ea boroko, lyisetonas aireus, klebriaas areus, klebriaa oxytoca Haemophilus Hursezae, Ackiibacter ea Ackiiphira, EstioneEm Fluollisia Monko, Shv, tem, joalo-joalo c) Ho kena-kenana le 'nete: Mucin, granicline, silika ho a ho lemoha Carbapenem rerication ea kpc, NDM, Oxa48, Oxa23, Vim, VIM. |
| Lisebelisoa tse sebetsang | E sebelisoa BIOSSYSTEMS 7500 Real-Nako ea Pruc Quantshetstudio®5 Real-Nako ea Likarolo Slan-96p Real-Nako ea 'nete ea £ Hongbili Medical Lightcycler®480 Real-Nako ea PCR ea Pru Linegene 9600 PLs Sistimi ea nako ea nako ea DRIT ea PRCEFQD-96A,HangzhouTheakch of Bioer) Ma-6000 Real-Nako ea nnete ea Thermal cycler (Suzhou Molayy Co, Ltd.) Biorad CFX96 Real-Nako ea Prust Biorad Cfx OPS 96 Real-Nako ea Prust |
Phallo ea mosebetsi
Khetho ea 1.
Kholiseho e khothalletsoang: Macro & Micro-Tector General Dna / Rna Kit (HWTS-3019-50, HWTS-3019-32, HWts-3019-48, HWts-3019-96) (e ka sebelisoang ka boits'oaro bo iketsahallang ka boiketsetso. prepitate ea Thallus. Mehato e latelang e lokela ho latela litaelo tsa ho tsoaloa, 'me Buka e khothalelitsoeng e100μl.
Khetho ea 2.
Ho khothalletsoa nako ea ho hlasimolla: Nucleic acident ea nyuger kapa ea porp302) ka tiangen biffction (Kenya 200 , le ho thothomela ho fihlela thallus e emisitsoe ka botlalo). Sebelisa sebaka sa rnase / DNAAse metsi a mahala bakeng sa khoheli, 'me Buka e theko e tlaase ea ho loka e hlahisa 100μl.
Khetho ea 3.
Khopolo e Nchafatso ea Entercon: Macro & Micro-Temple ea Khopolo ea Khopolo-taba. Mohlala oa SPUTUM o hloka ho hlatsuoa ka ho eketsa 1ml oa letsoai le tloaelehileng ho la 13000r / Min bakeng sa 100000. Bakeng sa swab ea kolone e hloekileng le ho thaba, eketsa 50μl ea sampole e ikhula ka tompole e boletsoeng ka holimo, mme mehato e latelang e lokela ho etsoa ho latela thupelo ea ts'ebeliso.
