Mefuta e 4 ea Likokoana-hloko tse Phefumolohang Nucleic Acid

Tlhaloso e Khutšoanyane:

Setsi sena se sebelisetsoa ho lemoha boleng ba SARS-CoV-2, kokoana-hloko ea ntaramane A, kokoana-hloko ea ntaramane ea B le li-nucleic acid tsa syncytial virus ka lisampole tsa batho ba oropharyngeal.

Re romelle lengolo-tsoibila

Lintlha tsa Sehlahisoa

Li-tag tsa Sehlahisoa

Lebitso la sehlahisoa

HWTS-RT075-Freeze-Omisitsoeng Mefuta e 4 ea Likokoana-hloko tse Phefumolohang Nucleic Acid Detection Kit (Fluorescence PCR)

Epidemiology

Lefu la Corona Virus 2019, le bitsoang "COVID-19", le bolela pneumonia e bakoang ke ts'oaetso ea SARS-CoV-2.SARS-CoV-2 ke coronavirus ea mofuta oa β.COVID-19 ke lefu le tšoaetsanoang le matla la phefumoloho, 'me baahi ba bangata hangata ba kotsing.Hajoale, mohloli oa tšoaetso ke bakuli ba tšoaelitsoeng haholo ke SARS-CoV-2, mme batho ba tšoaelitsoeng ba asymptomatic le bona e ka ba mohloli oa tšoaetso.Ho ipapisitsoe le lipatlisiso tsa morao-rao tsa epidemiological, nako ea ho incubation ke matsatsi a 1-14, haholo matsatsi a 3-7.Feberu, ho khohlela ho omileng le mokhathala ke lipontšo tse ka sehloohong.Bakuli ba seng bakae ba ne ba e-na le matšoao a kang ho tsubella nko, nko e phallang, 'metso o bohloko, myalgia le letšollo, joalo-joalo.

Influenza, eo hangata e tsejoang e le "ntaramane", ke lefu le tšoaetsanoang le matla la ho hema le bakoang ke vaerase ea ntaramane.E tšoaetsanoa haholo.E fetisoa haholo ke ho khohlela le ho thimola.Hangata e hlaha nakong ea selemo le mariha.Likokoana-hloko tsa ntaramane li arotsoe ka ntaramane A (IFV A), ntaramane B (IFV B), le Influenza C (IFV C) mefuta e meraro, kaofela ke ea kokoana e khomarelang, e bakang lefu la batho haholo-holo bakeng sa likokoana-hloko tsa ntaramane A le B, ke mofuta o le mong. kokoana-hloko ea RNA e arohaneng, e arohaneng.Kokoana-hloko ea Influenza A ke ts'oaetso e matla ea ho hema, e kenyeletsang H1N1, H3N2 le mefuta e meng e nyenyane, e atisang ho fetoha le ho qhoma lefatšeng ka bophara."Shift" e bolela phetoho ea kokoana-hloko ea ntaramane A, e hlahisang ho hlaha ha kokoana-hloko e ncha "subtype".Livaerase tsa mokakallane oa B li arotsoe ka mela e 'meli, Yamagata le Victoria.Kokoana-hloko ea Influenza B e na le ho hoholeha ha antijeni, 'me e qoba ho lekoa le ho felisoa ha sesole sa 'mele oa motho ka phetoho ea eona.Leha ho le joalo, lebelo la ho iphetola ha lintho la kokoana-hloko ea ntaramane ea B le butle ho feta ba kokoana-hloko ea ntaramane A.Kokoana-hloko ea Influenza B e ka boela ea baka mafu a matšoafo a batho 'me ea baka mafu a seoa.

Respiratory syncytial virus (RSV) ke kokoana-hloko ea RNA, ea lelapa la paramyxoviridae.E fetisoa ke marotholi a moea le ho kopana haufi-ufi 'me ke eona kokoana-hloko e ka sehloohong ea tšoaetso e tlaase ea matšoafo ho masea.Masea a tšoaelitsoeng ke RSV a ka ’na a ba le bronchiolitis e matla le pneumonia, tse amanang le asma ho bana.Masea a na le matšoao a matla, ho kenyelletsa feberu e phahameng, rhinitis, pharyngitis le laryngitis, ebe joale la bronchiolitis le pneumonia.Bana ba seng bakae ba kulang ba ka ba thata le otitis media, pleurisy le myocarditis, joalo-joalo Tšoaetso ea phefumoloho e ka holimo ke letšoao le ka sehloohong la tšoaetso ho batho ba baholo le bana ba baholo.

Channel

FAM	SARS-CoV-2
VIC(HEX)	RSV
CY5	IFV A
ROX	IFV B
NED	Taolo ea ka hare

Tekheniki Parameters

Polokelo	2-8°C
Shelf-bophelo	Likhoeli tse 12
Mofuta oa Mohlala	Oropharyngeal swab
Ct	≤38
LoD	SARS-CoV-2: 150Copies/mL Kokoana-hloko ea Influenza A/Kokoana-hloko ea Mofoka B/Vaerase ea ho hema ea syncytial: 300Copies/mL
Tse khethehileng	Ha ho cross-reactivity with human coronavirus SARSr-CoV, MERSr-CoV, HCoV-OC43, HCoV-229E, HCoV-HKU1, HCoV-NL63, parainfluenza virus type 1, 2, 3, rhinovirus A, B, C, chlamydia pneumoniae, human metapneumovirus, enterovirus A, B, C, D, kokoana-hloko ea matšoafo ea motho, kokoana-hloko ea epstein-barr, kokoana-hloko ea maselese, kokoana-hloko ea cytomegalo, rotavirus, norovirus, parotitis virus, varicella-zoster virus, legionella, bordetella pertussis, haemophilus influenzae, staphylococcus aureus, streptococcus pneumoniae, s.pyogenes, klebsiella pneumoniae, lefuba la mycobacterium, aspergillus ea mosi, candida albicans, candida glabrata, pneumocystis jiroveci le cryptococcus e sa tsoa tsoaloa le acid ea motho ea genomic nucleic.
Lisebelisoa tse sebetsang	Applied Biosystems 7500 Real-Time PCR System, Applied Biosystems 7500 Fast Real-Time PCR Systems, QuantStudio®5 Real-Time PCR Systems

Phallo ea Mosebetsi

Khetho ea 1.

Sehlahisoa sa ho ntša se khothalelitsoeng: Macro & Micro-Test Viral DNA/RNA Kit(HWTS-3001, HWTS-3004-32, HWTS-3004-48) le Macro & Micro-Test Automatic Nucleic Acid Extractor(HWTS-3006).

Khetho ea 2.

Sehlahisoa se khothalelitsoeng sa ho ntšoa: Nucleic Acid Extraction kapa Purification Reagent(YDP302) ke Tiangen Biotech(Beijing) Co.,Ltd.

E fetileng: Mycobacterium Tuberculosis Rifampicin Resistance

E 'ngoe: 19 Mefuta ea Phefumoloho ea Pathogen Nucleic Acid

Ngola molaetsa wa hao mona mme o re romele wona