Mefuta e 28 ea Papillomavirus ea Motho (16/18/45Typing) Kit ea ho Fumana Nucleic Acid (Fluorescence PCR)
Lebitso la sehlahisoa
Mefuta e 28 ea Papillomavirus ea Motho (16/18/45Typing) Kit ea ho Fumana Nucleic Acid (Fluorescence PCR)
Thuto ea mafu a seoa
Vaerase ea Papillomavirus ea Motho (HPV) ke ea lelapa la Papillomaviridae la kokoana-hloko ea DNA e nyenyane, e sa koaheloang, e chitja e nang le mela e 'meli, e bolelele ba genome ea lipara tse ka bang 8000 tsa motheo (bp). HPV e tšoaetsa batho ka ho kopana ka kotloloho kapa ka tsela e sa tobang le lintho tse silafetseng kapa phetiso ea thobalano. Vaerase ha e amane feela le moamoheli, empa hape e amana le lisele, 'me e ka tšoaetsa letlalo la motho le lisele tsa epithelial tsa mucosal, e leng se bakang mefuta e fapaneng ea li-papilloma kapa li-warts letlalong la motho le tšenyo e atileng ho epithelium ea tsela ea ho ikatisa.
Kankere ea molomo oa popelo ke e 'ngoe ea lihlahala tse kotsi tse atileng haholo tsa tsela ea ho ikatisa ea basali. Lithuto li bontšitse hore tšoaetso e sa khaotseng ea HPV le tšoaetso e mengata ke e 'ngoe ea lisosa tse ka sehloohong tsa kankere ea molomo oa popelo. Hona joale, mekhoa ea phekolo e sebetsang e tsebahalang e ntse e haella bakeng sa kankere ea molomo oa popelo e bakoang ke HPV, kahoo ho sibolloa le ho thibeloa ha tšoaetso ea molomo oa popelo e bakoang ke HPV ke senotlolo sa ho thibela kankere ea molomo oa popelo. Ho bohlokoa haholo ho theha tlhahlobo e bonolo, e tobileng le e potlakileng ea tlhahlobo ea etiology bakeng sa tlhahlobo ea kliniki le kalafo ea kankere ea molomo oa popelo.
Li-parameter tsa Tekheniki
| Polokelo | ≤ -18℃ |
| Nako ea ho phela | Likhoeli tse 12 |
| Mofuta oa Sampole | Urinesampole,Fsampole ea swab ea molomo oa popelo ea emale, Fsampole ea swab ea botšehali ea emale |
| CV | <5.0% |
| LoD | Moeli oa ho Lemoha (LoD): LoD ea kit ke 300Cli-opioid/mL |
| Ho hlaka | Ha ho na karabelo e fapanengka likokoana-hloko tse tšoanang. |
| Lisebelisoa tse Sebetsang | Sistimi ea PCR ea Applied Biosystems 7500 ea Nako ea 'Nete, Sistimi ea PCR ea Applied Biosystems 7500 e Potlakileng ea Nako ea 'Nete, QuantStudio®5 Sistimi ea PCR ea Nako ea 'Nete, Sistimi ea PCR ea SLAN-96P ea Nako ea 'Nete (Hongshi Medical Technology Co., Ltd.), LightCycler®480 Sistimi ea PCR ea Nako ea 'Nete, LineGene 9600 Plus Sistimi ea ho Fumana PCR ea Nako ea 'Nete (FQD-96A,HangzhouTheknoloji ea Bioer), MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.), BioRad CFX96 Real-Time PCR System le BioRad CFX Opus 96 Real-Time PCR System.
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Mokero:
| Sesebediswa sa ho Buffer sa Karabelo | Fluorophore | Mochini oa ho tima |
| PCR-Mix1/PCR-Mix2 | FAM | ha ho letho |
| VIC(HEX) | ha ho letho | |
| ROX | ha ho letho | |
| CY5 | ha ho letho |
Phallo ea mosebetsi: Sheba Kit IFU bakeng sa lintlha tse qaqileng
Tharollo ea 1 (Ho ntšoa ha nucleic acid ka boiketsetso)
Tharollo ea 2: (Sesebelisoa sa ho Lokolla Sampole ea Macro le Micro-Test)
Li-reagent li hloka empa ha lia fanoa:Sesebediswa sa ho Lokolla Sample Release Reagent sa Macro & Micro-Test (HWTS-3005-8), Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (e ka sebediswang le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) ke Jiangsu Macro & Micro-Test Med-Tech Co., Ltd., Macro & Micro-Test Viral DNA/RNA Column (HWTS-3020-50).
Lintho tse sebelisoang lia hlokahala empa ha lia fanoa: 1.5mL DNase/RNase ha e nacentrifugetlibese,DNase/RMaqheka a se nang Nase, 0.2mL ea lipeipi tsa PCR, centrifuge ea desktop, motsoako oa ho sisinyeha ha desktop.
