Mefuta e 28 ea Kotsi e Phahameng ea Papilloma Virus ea Motho (Typing ea 16/18) Nucleic Acid

Tlhaloso e Khutšoanyane:

Sesebelisoa sena se loketse ho lemoha boleng ba in vitro mefuta e 28 ea livaerase tsa papilloma ea motho (HPV6, 11, 16, 18, 26, 31, 33, 35, 39, 40, 42, 43, 44, 45, 51, 3, 55, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5. 61, 66, 68, 73, 81, 82, 83) nucleic acid ka moroto oa banna / basali le lisele tse tšehali tsa cervical exfoliated. HPV 16/18 e ka thaepa, mefuta e setseng e ke ke ea ngoloa ka ho feletseng, ho fana ka mokhoa o thusang bakeng sa tlhahlobo le phekolo ea tšoaetso ea HPV.

Re romelle lengolo-tsoibila

Lintlha tsa Sehlahisoa

Li-tag tsa Sehlahisoa

Lebitso la sehlahisoa

HWTS-CC006A-28 Mefuta ea Kotsi e Phahameng ea Papilloma Virus ea Motho (16/18 Typing) Nucleic Acid Detection Kit (Fluorescence PCR)

Epidemiology

Kankere ea popelo ke e 'ngoe ea lihlahala tse kotsi tse atileng ka ho fetesisa lesakeng la pelehi la mosali. Liphuputso li bontšitse hore tšoaetso ea HPV e phehellang le mafu a mangata ke e 'ngoe ea lisosa tse ka sehloohong tsa mofetše oa molomo oa popelo. Hajoale, liphekolo tse lemohuoeng tse sebetsang hantle li ntse li haella bakeng sa mofetše oa popelo o bakoang ke HPV, kahoo ho sibolloa kapele le thibelo ea tšoaetso ea molomo oa popelo e bakoang ke HPV ke senotlolo sa ho thibela mofetše oa popelo. Ho bohlokoa haholo ho theha tlhahlobo e bonolo, e tobileng le e potlakileng ea tlhahlobo ea etiology bakeng sa tlhahlobo ea bongaka le kalafo ea mofetše oa popelo.

Channel

Reaction Mix	Channel	Mofuta
PCR-Mix1	FAM	18
	VIC(HEX)	16
	ROX	31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, 68
	CY5	Taolo ea ka hare
PCR-Mix2	FAM	6, 11, 54, 83
	VIC(HEX)	26, 44, 61, 81
	ROX	40, 42, 43, 53, 73, 82
	CY5	Taolo ea ka hare

Tekheniki Parameters

Polokelo	Mokelikeli: ≤-18℃
Shelf-bophelo	Likhoeli tse 12
Mofuta oa Mohlala	Motsoako oa Cervical Swab, Vaginal Swab, Moroto
Ct	≤28
CV	<5.0%
LoD	300Likopi / mL
Lisebelisoa tse sebetsang	Applied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio^®Litsamaiso tse 5 tsa nako ea 'nete tsa PCR Litsamaiso tsa PCR tsa nako ea sebele tsa SLAN-96P LightCycler^®Sistimi ea 480 ea Nako ea 'Nete ea PCR LineGene 9600 Plus Nako ea 'Nete ea PCR ea ho Fumana Sistimi MA-6000 Real-Nako Quantitative Thermal Cycler Sistimi ea PCR ea Nako ea 'Nete ea BioRad CFX96 BioRad CFX Opus 96 Real-Nako PCR System

Phallo ea Mosebetsi

Sehlahisoa se khothalelitsoeng: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (e ka sebelisoang ka Macro & Micro-Test Automatic Nucleic Acid006W Extra HWTS-3006B)) ka Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Eketsa 200μL ea saline e tloaelehileng ho khutlisetsa pellet mohatong oa 2.1, ebe ho ntšoa ho lokela ho etsoa ho latela litaelo tsa tšebeliso ea reagent ena ea ho ntša. Molumo o khothaletsoang oa elution ke 80μL.
Sehlahisoa se khothalelitsoeng: QIAamp DNA Mini Kit (51304) kapa Macro & Micro-Test Viral DNA/RNA Column (HWTS-3020-50). Eketsa 200μL ea saline e tloaelehileng ho emisa pellet mohatong oa 2.1, 'me joale ho ntšoa ho lokela ho etsoa ho latela litaelo tsa tšebeliso ea reagent ena. Bolumo ea sampole e ntšitsoeng ea lisampole kaofela ke 200μL, 'me bophahamo ba molumo o khothaletsoang ke 100μL.

E fetileng: Enterococcus e hanyetsanang le Vancomycin le Gene e hanyetsanang le Lithethefatsi

E 'ngoe: Papillomavirus ea Motho (Mefuta e 28) Genotyping

Ngola molaetsa wa hao mona mme o re romele wona