Mefuta e 28 ea Vaerase ea Papilloma ea Motho e Kotsing e Phahameng (Type ea 16/18) Nucleic Acid
Lebitso la sehlahisoa
HWTS-CC006A-28 Mefuta ea Vaerase ea Papilloma ea Motho e Kotsing e Holimo (Typing ea 16/18) Kit ea ho Fumana Asiti ea Nucleic (Fluorescence PCR)
Thuto ea mafu a seoa
Kankere ea molomo oa popelo ke e 'ngoe ea lihlahala tse kotsi tse atileng haholo tsa tsela ea ho ikatisa ea basali. Lithuto li bontšitse hore tšoaetso e sa khaotseng ea HPV le tšoaetso e mengata ke e 'ngoe ea lisosa tse ka sehloohong tsa kankere ea molomo oa popelo. Hona joale, mekhoa ea phekolo e sebetsang e tsebahalang e ntse e haella bakeng sa kankere ea molomo oa popelo e bakoang ke HPV, kahoo ho sibolloa le ho thibeloa ha tšoaetso ea molomo oa popelo e bakoang ke HPV ke senotlolo sa ho thibela kankere ea molomo oa popelo. Ho bohlokoa haholo ho theha tlhahlobo e bonolo, e tobileng le e potlakileng ea tlhahlobo ea etiology bakeng sa tlhahlobo ea kliniki le kalafo ea kankere ea molomo oa popelo.
Mokero
| Motsoako oa Karabelo | Mokero | Mofuta |
| PCR-Mix1 | FAM | 18 |
| VIC(HEX) | 16 | |
| ROX | 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, 68 | |
| CY5 | Taolo ea Ka Hare | |
| PCR-Mix2 | FAM | 6, 11, 54, 83 |
| VIC(HEX) | 26, 44, 61, 81 | |
| ROX | 40, 42, 43, 53, 73, 82 | |
| CY5 | Taolo ea Ka Hare |
Li-parameter tsa Tekheniki
| Polokelo | Metsi: ≤-18℃ |
| Nako ea ho phela | Likhoeli tse 12 |
| Mofuta oa Sampole | Sesepa sa molomo oa popelo, Sesepa sa botšehali, moroto |
| Ct | ≤28 |
| CV | <5.0% |
| LoD | Likopi tse 300/mL |
| Lisebelisoa tse Sebetsang | Mekhoa ea Li-biosystem tse Sebelisitsoeng 7500 Mekhoa ea PCR ea Nako ea Sebele Mekhoa ea PCR e Potlakileng ea Li-Biosystem tse 7500 tsa Nako ea 'Nete QuantStudio®Mekhoa e 5 ea PCR ea Nako ea Sebele Mekhoa ea PCR ea Nako ea Sebele ea SLAN-96P Leseli la ho Tsamaisa Li-Cycler®Sistimi ea PCR ea Nako ea 'Nete ea 480 Sistimi ea ho lemoha PCR ea LineGene 9600 Plus ea Nako ea 'Nete MA-6000 Nako ea Sebele ea Bongata ba Thermal Cycler Sistimi ea PCR ea BioRad CFX96 ea Nako ea 'Nete Sistimi ea PCR ea BioRad CFX Opus 96 ea Nako ea 'Nete |
Phallo ea Mosebetsi
Reagent e kgothalletswang ya ho ntsha: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (e ka sebediswang le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) ke Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Kenya 200μL ya saline e tlwaelehileng ho tjhesa pellet hape mohatong wa 2.1, ebe ho ntsha ho lokela ho etswa ho ya ka ditaelo tsa tshebediso ya reagent ena ya ho ntsha. Bophahamo bo kgothaletswang ba ho hlakisa ke 80μL.
Reagent e kgothalletswang ho ntsha: QIAamp DNA Mini Kit (51304) kapa Macro & Micro-Test Viral DNA/RNA Column (HWTS-3020-50). Kenya 200μL ya letsoai le tlwaelehileng ho kgutlisa pellet mohatong wa 2.1, ebe ho ntsha ho lokela ho etswa ho ya ka ditaelo tsa tshebediso ya reagent ena ya ho ntsha. Bongata ba sampole e ntshitsweng ba disampole kaofela ke 200μL, mme bongata bo kgothaletswang ba ho hlatswa ke 100μL.
