Mefuta e 15 ea Papillomavirus ea Motho e Kotsing e Phahameng E6/E7 Gene mRNA
Lebitso la sehlahisoa
Mefuta ea HWTS-CC005A-15 ea Kit ea ho Fumana Gene mRNA ea Papillomavirus ea Motho e Kotsing e Holimo E6/E7 (Fluorescence PCR)
Thuto ea mafu a seoa
Kankere ea molomo oa popelo ke e 'ngoe ea mefuta e atileng haholo ea kankere ea basali lefatšeng ka bophara, 'me ho hlaha ha eona ho amana haufi-ufi le livaerase tsa papilloma tsa batho (HPV), empa ke karolo e nyane feela ea tšoaetso ea HPV e ka fetohang kankere. HPV e kotsi haholo e tšoaetsa lisele tsa epithelial tsa molomo oa popelo 'me e hlahisa liprotheine tse peli tsa oncoprotein, E6 le E7. Protheine ena e ka ama mefuta e fapaneng ea liprotheine tsa sele (joalo ka liprotheine tse thibelang hlahala pRB le p53), ea lelefatsa potoloho ea sele, ea ama tlhahiso ea DNA le botsitso ba genome, 'me ea sitisa likarabelo tsa' mele tsa ho itšireletsa mafung le tse khahlanong le hlahala.
Mokero
| Mokero | Karolo | Tlhahlobo ea liphatsa tsa lefutso |
| FAM | Sesebediswa sa ho thibela ho ata ha HPV 1 | HPV16, 31, 33, 35, 51, 52, 58 |
| VIC/HEX | Jini ea β-actin ea motho | |
| FAM | Sesebediswa sa ho thibela ho ata ha HPV 2 | HPV 18, 39, 45, 53, 56, 59, 66, 68 |
| VIC/HEX | Lefutso la INS la motho |
Li-parameter tsa Tekheniki
| Polokelo | Metsi: ≤-18℃ |
| Nako ea ho phela | Likhoeli tse 9 |
| Mofuta oa Sampole | Sesepa sa molomo oa popelo |
| Ct | ≤38 |
| CV | <5.0% |
| LoD | Likopi tse 500/mL |
| Lisebelisoa tse Sebetsang | Sistimi ea PCR ea Nako ea 'Nete e Sebelisitsoeng ea 7500Mekhoa ea PCR e Potlakileng ea Li-Biosystem tse 7500 tsa Nako ea 'Nete Mekhoa ea PCR ea QuantStudio®5 ea Nako ea 'Nete Mekhoa ea PCR ea Nako ea Sebele ea SLAN-96P Sistimi ea PCR ea LightCycler®480 ea Nako ea 'Nete Sistimi ea ho lemoha PCR ea LineGene 9600 Plus ea Nako ea 'Nete MA-6000 Nako ea Sebele ea Bongata ba Thermal Cycler Sistimi ea PCR ea BioRad CFX96 ea Nako ea 'Nete Sistimi ea PCR ea BioRad CFX Opus 96 ea Nako ea 'Nete |
Phallo ea Mosebetsi
Reagent e kgothalletswang ho ntsha: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3020-50-HPV15) ka Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ho ntsha ho lokela ho etswa ka thata ho ya ka ditaelo tsa tshebediso. Bophahamo bo kgothaletswang ba ho ntsha ke 50μL. Haeba sampole e sa silwa ka botlalo, e kgutlisetse mohatong wa 4 bakeng sa ho ntsha metsi hape. Ebe o leka ho ya ka ditaelo tsa tshebediso.
Reagent e kgothalletswang ya ho ntsha: RNAprep Pure Animal Tissue Total RNA Extraction Kit (DP431). Ho ntsha ho lokela ho etswa ho ya ka ditaelo tsa tshebediso ka tieo (Mohatong wa 5, eketsa habeli mahloriso a tharollo e sebetsang ya DNaseI, ke hore, nka 20μL ya tharollo ya setoko ya RNase-Free DNaseI (1500U) ka hara tube e ntjha ya RNase-Free centrifuge, eketsa 60μL ya RDD buffer, mme o kopanye butle). Bophahamo bo kgothaletswang ba ho ntsha ke 60μL. Haeba sampole e sa silwa ka botlalo, e kgutlisetse mohatong wa 5 bakeng sa ho ntsha metsi hape. Ebe o leka ho ya ka ditaelo tsa tshebediso.
