Mefuta e 15 ea Litorillomavirus ea batho e kotsing e kholo e6 / e7 Gener 'mirna
Lebitso la Product
HWTS-CC005a-CC00A-15 Mefuta ea PapilloMuvisuvirus ea Batho ea Papillomavirus ea batho ba 6 / e7 Gener Kit (fluorescence pcr)
Epidphiology
Kankere ea Cervical ke e 'ngoe ea masholu a atileng haholo ea mofets'e o atileng haholo oa mofets'e o amanang haufi le mofetše oa hae oa papillomadumps. HPV e kotsing e kholo ea HPV e tšoantšitsoeng ke lisele tsa maiketsetso e kholo ea lisele tse peli 'me li hlahisa li-oncoteins tse peli, E6 le E7. Liprotheine ena e ka ama li-s findsor tsa ho hatella prb le p53), phetoloha li-dna synthesis le tsitsipano ea li-antiviral.
Channel
| Channel | Karolo | Geotype e lekoa |
| FMAM | HPV Uaction Buffer 1 | HPV16,313,35 71,58 58,58 58 |
| Video / Hex | Monghali β-β gene | |
| FMAM | HPV Uay Buffer 2 | Hpv 18,39,55,56,66,66,66,66,66,66,66 |
| Video / Hex | Kholo ea motho |
Litekanyetso tsa tekheniki
| Polokelo | Metsi: ≤-18 ℃ |
| Shell-Bophelo | Likhoeli tse 9 |
| Mofuta oa SIMON | Swab ea cervical |
| Ct | ≤38 |
| CV | <5.0% |
| Lod | 500 likopi / ml |
| Lisebelisoa tse sebetsang | E sebelisoa habeli BiosSetems 7500 Real-Nako ea Nako ea Bobeli ea PCRBiosstems ea BiosStems 7500 ea Nako e sa Ts'oerehileng ea Nako ea Nako ea Nako Quantshetstudio®5 Real-Nako ea Likarolo Slan-96p Real-Nako ea Likarolo tsa nako ea ly-Nako Lightcycler®480 Real-Nako ea PCR ea Pru Linegene 9600 le sistimi ea nako ea nako ea nako ea nako Ma-6000 ea 'nete ea The Larmal Cyclement Cyclement Biorad CFX96 Real-Nako ea Prust Biorad Cfx OPS 96 Real-Nako ea Prust |
Phallo ea mosebetsi
E khothalelitsoeng e behang: Macro & Micro-Teb-Tem-Tem-Pro Veraral DNA / HU KIT (HWTS . Molumo o khothalletsoang oa ho Emela ke 50μl. Haeba sampole ha e fokotsoe ka botlalo, e khutlisetse ho isa mohatong oa 4 bakeng sa ho khutlisa. Ebe u leka ho latela litaelo tseo u ka li sebelisang.
Kholiso e khothalelitsoeng: Rnaprep e hloekileng ea liphoofolo tse hloekileng tsa Tissue Kakaretso ea Rna Dna Exction KNA (DP431). Ts'ebetso e lokela ho etsoa ho latela litaelo tsa ho sebelisa ka tieo (mohato oa bohlano, habeli khaello ea lithuso tsa Rnase-Free Dnase-Freerifuge Tube, Kenya 60μl ea RDD Buffer, 'me u kopanya ka bonolo). Buka e khothalelitsoeng ea ho lumelisa e na le 60μl. Haeba sampole e sa khangoa ka botlalo, e khutlisetse ka mohato oa 5 bakeng sa ho nchafatsa. Ebe u leka ho latela litaelo tseo u ka li sebelisang.
