West Nile Virus Nucleic Acid
Lebitso la sehlahisoa
HWTS-FE041-West Nile Virus Nucleic Acid Detection Kit(Fluorescence PCR)
Epidemiology
Kokoana ea West Nile ke setho sa lelapa la Flaviviridae, mofuta oa Flavivirus, 'me e le sehlopheng se le seng le kokoana-hloko ea encephalitis ea Japane, kokoana-hloko ea dengue, kokoana-hloko ea yellow fever, kokoana-hloko ea St. Kokoana-hloko ea West Nile e fetisoa ka linonyana e le li-reservoir hosts, 'me batho ba tšoaetsoa ke ho longoa ke menoang ea ho fepa linonyana (ornithophilic) joalo ka Culex. Batho, lipere le liphoofolo tse ling tse anyesang lia kula ka mor’a ho longoa ke menoang e nang le kokoana-hloko ea West Nile. Maemo a bobebe a ka hlaha ka matšoao a kang a ntaramane joalo ka feberu le hlooho, ha maemo a matla a ka hlaha ka matšoao a tsamaiso ea methapo kapa lefu[1-3]. Lilemong tsa morao tjena, ka lebaka la ho teba ha lipuisano le tšebelisano ea machaba, lipuisano pakeng tsa linaha li fetohile khafetsa, 'me palo ea baeti e ntse e eketseha selemo le selemo. Ka nako e ts'oanang, ka lebaka la lintlha tse kang ho falla ha linonyana tse fallang, monyetla oa hore feberu ea West Nile e kenngoe Chaena e eketsehile[4].
Tekheniki Parameters
| Polokelo | -18℃ |
| Shelf-bophelo | Likhoeli tse 12 |
| Mofuta oa Mohlala | lisampole tsa serum |
| CV | ≤5.0% |
| LoD | 500 Likopi / μL |
| Lisebelisoa tse sebetsang | E sebetsa ho mochini oa ho lemoha oa mofuta oa I: Applied Biosystems 7500 Real-Nako PCR Systems, QuantStudio®Litsamaiso tse 5 tsa nako ea 'nete tsa PCR, SLAN-96P ea Nako ea Sebele PCR Systems (Hongshi Medical Technology Co., Ltd.), LineGene 9600 Plus Nako ea 'Nete ea PCR Detection Systems (FQD-96A, theknoloji ea Hangzhou Bioer), MA-6000 Real-Nako Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.), Sistimi ea PCR ea Nako ea 'Nete ea BioRad CFX96, BioRad CFX Opus 96 Real-Nako PCR System. E sebetsa ho mofuta oa II reagent ea ho lemoha: EudemonTMAIO800 (HWTS-EQ007) ka Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. |
Phallo ea Mosebetsi
Khetho ea 1.
Sehlahisoa sa ho ntša se khothalelitsoeng: Macro & Micro-Test Viral DNA/RNA Kit(HWTS-3001, HWTS-3004-32, HWTS-3004-48) le Macro & Micro-Test Nucleic Acid Extractor(HWTS-3006).
Khetho ea 2.
Sehlahisoa se khothalelitsoeng sa ho ntšoa: Nucleic Acid Extraction or Purification Kit(YD315-R) e entsoeng ke Tiangen Biotech(Beijing) Co., Ltd.
