Kiti ea ho lemoha Staphylococcus Aureus le Staphylococcus Aureus Nucleic Acid e Hanelang Methicillin
Lebitso la sehlahisoa
Kit ea ho lemoha Staphylococcus Aureus le Staphylococcus Aureus e Hanelang Methicillin (Fluorescence PCR)
Tšebeliso e Reriloeng
Sete ena e sebelisetsoa ho fumana boleng ba staphylococcus aureus le staphylococcus aureus nucleic acids e hananang le methicillin lisampoleng tsa sputum tsa batho, lisampoleng tsa swab ea nko le lisampoleng tsa tšoaetso ea letlalo le lisele tse bonolo ka har'a vitro. Sampole ea sputum le tlhahlobo ea sampole ea swab ea nko li sebelisoa litsing tsa bongaka ho thibela le ho laola tšoaetso ea MRSA nosocomial ho bakuli ba sepetlele, ho kenyeletsoa bakuli ba tlhokomelo e matla, bakuli ba opereishene le bakuli ba tlhokomelo ea nako e telele; E ka boela ea sebelisoa bakeng sa tlhahlobo e thusang ea bakuli ba belaelloang ba tšoaetso ea phefumoloho ea Staphylococcus Aureus le Staphylococcus Aureus e hananang le Methicillin; Kapa hammoho le liteko tse ling tsa laboratori tse kang litso tsa likokoana-hloko ho thusa ho fumaneng tšoaetso ea letlalo le lisele tse bonolo tsa MRSA/SA. Liphetho tsa liteko ha li sebelisoe ka kotloloho ho tataisa tšebeliso ea lithibela-mafu.
Thuto ea mafu a seoa
Staphylococcus aureus ke e 'ngoe ea libaktheria tsa bohlokoa tse bakang tšoaetso ea nosocomial. Staphylococcus aureus (SA) ke ea staphylococcus 'me ke moemeli oa libaktheria tse nang le Gram-positive, tse ka hlahisang chefo e fapaneng le li-enzyme tse hlaselang. Libaktheria li na le litšobotsi tsa kabo e pharaletseng, ho kula ho matla le sekhahla se phahameng sa ho hanyetsa. Lefu la nuclease le nang le thermostable (nuc) ke lefu la staphylococcus aureus le bolokiloeng haholo.
Lilemong tsa morao tjena, ka lebaka la tšebeliso e pharaletseng ea lihormone le litokisetso tsa 'mele oa ho itšireletsa mafung le tšebeliso e mpe ea lithibela-mafu tse nang le mahlakore a mangata, tšoaetso ea nosocomial e bakoang ke Staphylococcus aureus (MRSA) e hanyetsanang le Methicillin ho Staphylococcus e ntse e eketseha. Sekhahla sa naha sa ho lemoha MRSA e ne e le 30.2% ka 2019 Chaena. MRSA e arotsoe ka MRSA (HA-MRSA) e amanang le tlhokomelo ea bophelo bo botle, MRSA (CA-MRSA) e amanang le sechaba, le MRSA (LA-MRSA) e amanang le liphoofolo. CA-MRSA, HA-MRSA, LA-MRSA li na le liphapang tse kholo ho microbiology, ho hanyetsa baktheria (mohlala, HA-MRSA e bontša ho hanyetsa meriana e mengata ho feta CA-MRSA) le litšobotsi tsa kliniki (mohlala, sebaka sa tšoaetso). Ho ea ka litšobotsi tsena, CA-MRSA le HA-MRSA li ka khetholloa. Leha ho le joalo, liphapang pakeng tsa CA-MRSA le HA-MRSA lia fokotseha ka lebaka la motsamao o sa khaotseng oa batho lipakeng tsa lipetlele le metse. MRSA e hanela meriana e mengata, ha e hanele feela lithibela-mafu tsa β-lactam, empa hape e hanela le aminoglycosides, macrolides, tetracyclines le quinolones ka tekanyo e fapaneng. Ho na le liphapang tse kholo tsa libaka tsa sekhahla sa ho hanyetsa meriana le mekhoa e fapaneng. MRSA e ka hlasela 'mele ka maqeba a letlalo, li-follicle tsa moriri, mali le lisosa tse ling tsa tšoaetso ea suppurative,' me bakuli ba nang le mafu a letlalo le ho chesa ba kotsing e kholo ea tšoaetso ea MRSA. Pneumonia ke e 'ngoe ea tšoaetso e tloaelehileng haholo ea kliniki ea MRSA, 'me kalafo e sa lokelang ke sesosa sa bohlokoa sa lefu le phahameng.
Li-parameter tsa Tekheniki
| Polokelo | ≤ -18℃ |
| Nako ea ho phela | Likhoeli tse 12 |
| Mofuta oa Sampole | Sekhohlela, Sesepa sa nko, Sampole ea tšoaetso ea letlalo le lisele tse bonolo, |
| CV | <5.0% |
| LoD | 1000 CFU/mL |
| Ho hlaka | Teko ea ho kopanya lisele e bontša hore kit ena ha e na mokhoa oa ho kopanya lisele le likokoana-hloko tse ling tse phefumolohang tse kang staphylococcus aureus e nang le methicillin, staphylococcus e se nang coagulase, staphylococcus epidermidis e hanyetsanang le methicillin, pseudomonas aeruginosa, escherichia coli, klebsiella pneumoniae, acinetobacter baumannii, proteus mirabilis, enterobacter cloacae, streptococcus pneumoniae, enterococcus faecium, candida albicans, legionella pneumophila, candida parapsilosis, moraxella catarrhalis, neisseria meningitidis, haemophilus influenzae. |
| Lisebelisoa tse Sebetsang | Mofuta oa I: Mekhoa ea Biosystems 7500 ea Nako ea 'Nete ea PCR, Mekhoa ea QuantStudio®5 ea Nako ea 'Nete ea PCR, Mekhoa ea SLAN-96P ea Nako ea 'Nete ea PCR (Hongshi Medical Technology Co., Ltd.), Sistimi ea ho Fumana PCR ea LineGene 9600 Plus ea Nako ea 'Nete ea ho Etela (FQD-96A, theknoloji ea Hangzhou Bioer), Sepalami sa Thermal sa MA-6000 sa Nako ea 'Nete sa Quantitative (Suzhou Molarray Co., Ltd.), Sistimi ea PCR ea BioRad CFX96 ea Nako ea 'Nete, Sistimi ea PCR ea BioRad CFX Opus 96 ea Nako ea 'Nete. Mofuta oa II: HWTS-EudemonTM AIO800 |
Phallo ea Mosebetsi
Li-reagent le lisebelisoa lia hlokahala empa ha lia fanoa
Mofuta oa I
(1) Kit ea DNA/RNA e Akaretsang ea Macro le Micro-Test (HWTS-3019) ho tsoa ho Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. (E ka sebelisoa le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006B, 3006C).
(2) Motsoako oa sampole: 4% NaOH kapa Sputasol ho tsoa ho Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. (STXB 20220253)
(3) Letswai le tloaelehileng.
Mofuta oa II:
Kit ea DNA/RNA e Akaretsang ea Macro le Micro-Test (HWTS-3019-8) ho tsoa ho Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.
Lintho tse sebelisoang lia hlokahala empa ha lia fanoa:Malebela a se nang RNase/DNase, Liatlana tse lahloang, li-tube tsa EP tse se nang RNase/DNase, li-strips tsa li-tube tse 8 bakeng sa PCR.
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