Staphylococcus Aureus le Staphylococcus Aureus e Hanelang Methicillin (MRSA/SA)
Lebitso la sehlahisoa
HWTS-OT062 Staphylococcus Aureus le Staphylococcus Aureus e Hanelang Methicillin (MRSA/SA) Nucleic Acid Detection Kit (Fluorescence PCR)
Setifikeiti
CE
Thuto ea mafu a seoa
Staphylococcus aureus ke e 'ngoe ea libaktheria tsa bohlokoa tse bakang tšoaetso ea nosocomial. Staphylococcus aureus (SA) ke ea staphylococcus 'me ke moemeli oa libaktheria tse nang le Gram-positive, tse ka hlahisang chefo e fapaneng le li-enzyme tse hlaselang. Libaktheria li na le litšobotsi tsa kabo e pharaletseng, ho kula ho matla le sekhahla se phahameng sa ho hanyetsa. Lefu la nuclease le nang le thermostable (nuc) ke lefu la staphylococcus aureus le bolokiloeng haholo.
Mokero
| FAM | jini ea mecA e hananang le methicillin |
| ROX | Taolo ea Ka Hare |
| CY5 | jini ea nuc ea staphylococcus aureus |
Li-parameter tsa Tekheniki
| Polokelo | ≤-18℃ & e sirelelitsoe khanyeng |
| Nako ea ho phela | Likhoeli tse 12 |
| Mofuta oa Sampole | disampole tsa sekhohlela, letlalo le dinama tse bonolo tsa tshwaetso, le disampole tsa swab ya nko |
| Ct | ≤36 |
| CV | ≤5.0% |
| LoD | 1000 CFU/mL staphylococcus aureus, 1000 CFU/mL libaktheria tse hanelang methicillin. Ha kit e lemoha referense ea naha ea LoD, 1000/mL staphylococcus aureus e ka fumanoa |
| Ho hlaka | Teko ea ho kopanya lisele e bontša hore kit ena ha e na mokhoa oa ho kopanya lisele le likokoana-hloko tse ling tse phefumolohang tse kang staphylococcus aureus e nang le methicillin, staphylococcus e se nang coagulase, staphylococcus epidermidis e hanyetsanang le methicillin, pseudomonas aeruginosa, escherichia coli, klebsiella pneumoniae, acinetobacter baumannii, proteus mirabilis, enterobacter cloacae, streptococcus pneumoniae, enterococcus faecium, candida albicans, legionella pneumophila, candida parapsilosis, moraxella catarrhalis, neisseria meningitidis, haemophilus influenzae. |
| Lisebelisoa tse Sebetsang | Mekhoa ea Li-biosystem tse Sebelisitsoeng 7500 Mekhoa ea PCR ea Nako ea Sebele QuantStudio®Mekhoa e 5 ea PCR ea Nako ea Sebele Mekhoa ea PCR ea Nako ea Sebele ea SLAN-96P Leseli la ho Tsamaisa Li-Cycler®Sistimi ea PCR ea Nako ea 'Nete ea 480 Sistimi ea ho lemoha PCR ea LineGene 9600 Plus ea Nako ea 'Nete MA-6000 Nako ea Sebele ea Bongata ba Thermal Cycler Sistimi ea PCR ea BioRad CFX96 ea Nako ea 'Nete Sistimi ea PCR ea BioRad CFX Opus 96 ea Nako ea 'Nete |
Phallo ea Mosebetsi
Khetho ea 1.
Macro & Micro-Test Genomic DNA/RNA Kit (HWTS-3019) ea Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. e ka sebelisoa le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B). Kenya 200µL ea letsoai le tloaelehileng ho precipitate e sebetsitsoeng, 'me mehato e latelang e lokela ho ntšoa ho latela litaelo, 'me bophahamo bo khothaletsoang ba elution ke 80µL.
Khetho ea 2.
Setlolo sa ho Lokolla Sample Release Reagent sa Macro & Micro-Test (HWTS-3005-8) sa Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Kenya 1mL ea letsoai le tloaelehileng ho precipitate ka mor'a ho hlatsoa ka letsoai le tloaelehileng, ebe u kopanya hantle. Centrifuge ka 13,000r/min metsotso e 5, tlosa supernatant (boloka 10-20µL ea supernatant), 'me u latele litaelo tsa ho ntša metsi ka mor'a moo.
Reagent e kgothalletswang ho ntsha: Nucleic Acid Extraction kapa Purification Reagent (YDP302) ke Tiangen Biotech (Beijing) Co., Ltd. Ho ntsha ho lokela ho etswa ka thata ho latela mohato wa 2 wa bukana ya ditaelo. Ho kgothaletswa ho sebedisa metsi a se nang RNase le DNase bakeng sa ho ntsha metsi ka bophahamo ba 100µL.
