Staphylococcus Aureus le Methicillin-Resistant Staphylococcus Aureus (MRSA/SA)
Lebitso la sehlahisoa
HWTS-OT062 Staphylococcus Aureus le Methicillin-Resistant Staphylococcus Aureus (MRSA/SA) Nucleic Acid Detection Kit(Fluorescence PCR)
Setifikeiti
CE
Epidemiology
Staphylococcus aureus ke e 'ngoe ea libaktheria tsa bohlokoa tsa pathogenic tsa nosocomial.Staphylococcus aureus (SA) ke ea staphylococcus 'me ke moemeli oa baktheria ea Gram-positive, e ka hlahisang mefuta e mengata ea chefo le li-enzyme tse hlaselang.Libaktheria li na le litšobotsi tsa ho ajoa ka bophara, pathogenicity e matla le sekhahla se phahameng sa ho hanyetsa.Thermostable nuclease gene (nuc) ke mofuta o bolokehileng haholo oa staphylococcus aureus.
Channel
| FAM | Lefutso la mecA le hananang le methicillin |
| ROX | Taolo ea ka hare |
| CY5 | staphylococcus aureus nuc gene |
Tekheniki Parameters
| Polokelo | ≤-18℃ & e sirelelitsoe khanyeng |
| Shelf-bophelo | Likhoeli tse 12 |
| Mofuta oa Mohlala | sekhohlela, disampole tsa tshwaetso ya letlalo le dithishu tse bonolo, le disampole tsa swab ya nko |
| Ct | ≤36 |
| CV | ≤5.0% |
| LoD | 1000 CFU/mL staphylococcus aureus, 1000 CFU/mL libaktheria tse hanyetsanang le methicillin.Ha khiti e lemoha tšupiso ea naha ea LoD, 1000/mL staphylococcus aureus e ka bonoa. |
| Tse khethehileng | Teko e nang le litefo e bonts'a hore ke tlokotsi ena e be le tšusumetso ea li-pathoges tse ling tse ntle tse nang le limela, li-ascherichi li hatella. , Proteus mirabilis, enterobacter cloacae, streptococcus pneumoniae, enterococcus faecium, candida albicans, legionella pneumophila, candida parapsilosis, moraxella catarrhalis, neisseria meningitidis, haemophilus influenzae. |
| Lisebelisoa tse sebetsang | Applied Biosystems 7500 Real-Time PCR Systems QuantStudio®Litsamaiso tse 5 tsa nako ea 'nete tsa PCR Litsamaiso tsa PCR tsa nako ea sebele tsa SLAN-96P LightCycler®Sistimi ea 480 ea Nako ea 'Nete ea PCR LineGene 9600 Plus Nako ea 'Nete ea PCR ea ho Fumana Sistimi MA-6000 Real-Nako Quantitative Thermal Cycler Sistimi ea PCR ea Nako ea 'Nete ea BioRad CFX96 BioRad CFX Opus 96 Real-Nako PCR System |
Phallo ea Mosebetsi
Khetho ea 1.
Macro & Micro-Test Genomic DNA/RNA Kit (HWTS-3019) ka Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. e ka sebelisoa le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS- 3006B).Eketsa 200µL ea saline e tloaelehileng ho pula e sebelitsoeng, 'me mehato e latelang e lokela ho ntšoa ho ea ka litaelo,' me bophahamo ba molumo o khothaletsoang ke 80µL.
Khetho ea 2.
Macro & Micro-Test Sample Release Reagent (HWTS-3005-8) ka Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Kenya 1mL ea saline e tloaelehileng ho metsi ka mor'a ho hlatsoa ka saline e tloaelehileng, ebe u kopanya hantle.Centrifuge ho 13,000r/min bakeng sa metsotso e 5, tlosa supernatant (boloka 10-20µL ea supernatant), 'me u latele litaelo bakeng sa ho ntšoa ho latelang.
Sehlahisoa se khothalelitsoeng sa ho ntšoa: Nucleic Acid Extraction kapa Purification Reagent (YDP302) ke Tiangen Biotech (Beijing) Co.,Ltd.Ho ntšoa ho lokela ho etsoa ka tieo ho latela mohato oa 2 oa bukana ea litaelo.Ho khothaletsoa ho sebelisa metsi a se nang RNase le a DNase bakeng sa tlhahiso ea molumo oa 100µL.
