Likokoana-hloko tse Tšeletseng tsa ho Phefumoloha
Lebitso la sehlahisoa
HWTS-RT175-Six Pathogens Phefumolohang Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Mafu a phefumolohang ke sehlopha se atileng haholo sa mafu a batho a ka bang teng ho bong leha e le bofe, lilemo le sebaka sa libaka 'me ke e' ngoe ea lisosa tse kholo tsa ho kula le ho shoa ha baahi lefatšeng ka bophara. Likokoana-hloko tse tloaelehileng tsa ho hema li kenyelletsa kokoana ea ho hema ea syncytial, adenovirus, human metapneumovirus, rhinovirus, kokoana-hloko ea parainfluenza (I/II/III) le Mycoplasma pneumoniae. Matšoao a kliniki le matšoao a bakoang ke tšoaetso ea matšoafo a batla a tšoana, empa phekolo, katleho, le nako ea lefu lena li fapana pakeng tsa tšoaetso e bakoang ke likokoana-hloko tse fapaneng. Hona joale, mekhoa e ka sehloohong ea ho lemoha ka laboratoring ea likokoana-hloko tse ka holimo tsa phefumoloho e kenyelletsa: ho itšehla thajana ha kokoana-hloko, ho lemoha antigen le ho lemoha ha nucleic acid. Setsi sena se thusa ho hlahlobisisa tšoaetso ea kokoana-hloko ea ho hema ka ho lemoha le ho khetholla li-nucleic acid tse khethehileng ho batho ba nang le matšoao le matšoao a tšoaetso ea ho hema, hammoho le liphuputso tse ling tsa kliniki le tsa laboratori.
Tekheniki Parameters
| Polokelo | ≤-18℃ |
| Shelf-bophelo | Likhoeli tse 12 |
| Mofuta oa Mohlala | Mohlala oa oropharyngeal swab |
| Ct | Adv, PIV, MP, RhV, hMPV, RSV Ct≤38 |
| CV | <5.0% |
| LoD | LoD ea Adv, MP, RSV, hMPV, RhV le PIV kaofela ke 200Copies/mL |
| Tse khethehileng | Liphetho tsa tlhahlobo ea cross-reactivity li bonts'itse hore ha ho na cross-reactivity pakeng tsa kit le novel coronavirus, kokoana-hloko ea ntaramane A, kokoana-hloko ea ntaramane ea B, bocavirus ea motho, cytomegalovirus, herpes simplex virus mofuta oa 1, varicella zoster virus, EBV, pertussis bacillus, Chlamydophila pneumoniae, Corynebacteriosis, Ecolynebacterium cone, Heebacterium cone, Haemophilus Lactobacillus spp, Legionella pneumophila, C. catarrhalis, le mefuta e fokolisitsoeng ea Mycobacterium tuberculosis, Neisseria meningitidis, Neisseria spp, Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus epidermicoccus pneumoniae, St. Streptococcus salivarius, Actinobacillus baumannii, Narrow-feeding maltophilic monococci, Burkholderia maltophilia, Streptococcus striatus, Nocardia sp., Sarcophaga viscosa, Citrobacter citriodora, Cryptococcus spp, Aspertusflausgilluss, Aspertusflavusgilluss, Aspertus flacus, Aspertus, Aspertus, Aspertus, Aspertus, Aspertus, P, Nocardia sp. Candida albicans, Rohypnogonia viscera, oral streptococci, Klebsiella pneumoniae, Chlamydia psittaci, Rickettsia q fever le human genomic nucleic acids. Bokhoni ba ho thibela ho kena-kenana le: mucin (60 mg/mL), mali a motho, benfotiamine (2 mg/mL), oxymetazoline (2 mg/mL), sodium chloride (20 mg/mL), beclomethasone (20 mg/mL), dexamethasone (20 mg/mL), flunitrazolone/lomcinode (20 μcinode) mg/mL), budesonide (1 mg/mL), mometasone (2 mg/mL), fluticasone (2 mg/mL), histamine hydrochloride (5 mg/mL), intranasal live influenza virus vaccine, benzocaine (10%), menthol (10%), zanamivir (20 mg/mLvirin/mL), rianamivir (20 mg/mLvir1) oseltamivir (0.15 mg/mL), mupirocin (20 mg/mL), tobramycin (0.6 mg/mL), UTM, saline, guanidine hydrochloride (5 M/L), Tris (2 M/L), ENTA-2Na (0.6 M/L), trilostane (15%), isopropyl, potassium chloride (2%) le joala bo le bong (2) tlhahlobo ea tšitiso, liphetho li bontšitse hore ho ne ho se na karabelo ea tšitiso ho liphetho tsa ho sibolloa ha pathogen libakeng tse kaholimo tsa lintho tse sitisang. |
| Lisebelisoa tse sebetsang | Litsamaiso tsa PCR tsa nako ea sebele tsa SLAN-96P Lisebelisoa tsa Biosystems 7500 tsa Nako ea 'Nete ea PCR QuantStudio®Litsamaiso tse 5 tsa nako ea 'nete tsa PCR LineGene 9600 Plus Nako ea 'Nete ea PCR ea ho Fumana Sistimi MA-6000 Real-Nako Quantitative Thermal Cycler Sistimi ea PCR ea Nako ea 'Nete ea BioRad CFX96 BioRad CFX Opus 96 Real-Nako PCR System |
Phallo ea Mosebetsi
Macro & Micro-Test General DNA/RNA Kit (HWTS-3019) (e ka sebelisoang le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, (HWTS-3006B)ka Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. ba kgothaletswa bakeng sa mohlala ntsa lemehato e latelang e lokela ho batsamaisated ka tieo ho latela IFUea Kit.
