Mefuta e tšeletseng ea likokoana-hloko tsa phefumoloho

Tlhaloso e Khutšoanyane:

Sesebelisoa sena se ka sebelisoa ho lemoha ka mokhoa o nepahetseng nucleic acid ea SARS-CoV-2, kokoana-hloko ea ntaramane A, kokoana-hloko ea ntaramane ea B, adenovirus, mycoplasma pneumoniae le kokoana-hloko ea syncytial ea ho hema.

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HWTS-OT058A/B/C/Z-Real time fluorescent RT-PCR kit bakeng sa ho lemoha mefuta e tšeletseng ea likokoana-hloko tsa phefumoloho

Setifikeiti

Epidemiology

Lefu la Corona Virus 2019, le bitsoang "COVID-19", le bolela pneumonia e bakoang ke ts'oaetso ea SARS-CoV-2.SARS-CoV-2 ke coronavirus ea mofuta oa β.COVID-19 ke lefu le tšoaetsanoang le matla la phefumoloho, 'me baahi ba bangata hangata ba kotsing.Hajoale, mohloli oa tšoaetso ke bakuli ba tšoaelitsoeng haholo ke SARS-CoV-2, mme batho ba tšoaelitsoeng ba asymptomatic le bona e ka ba mohloli oa tšoaetso.Ho ipapisitsoe le lipatlisiso tsa morao-rao tsa epidemiological, nako ea ho incubation ke matsatsi a 1-14, haholo matsatsi a 3-7.Feberu, ho khohlela ho omileng le mokhathala ke lipontšo tse ka sehloohong.Bakuli ba seng bakae ba ne ba e-na le tšubuhlellano ea nko, nko e phallang, 'metso o bohloko, myalgia le letšollo.

Influenza, eo hangata e tsejoang e le "ntaramane", ke lefu le tšoaetsanoang le matla la ho hema le bakoang ke vaerase ea ntaramane.E tšoaetsanoa haholo.E fetisoa haholo ke ho khohlela le ho thimola.Hangata e hlaha nakong ea selemo le mariha.Likokoana-hloko tsa ntaramane li arotsoe ka ntaramane A, IFV A, ntaramane B, IFV B, le Influenza C, IFV C mefuta e meraro, kaofela ke ea kokoana-hloko e khomarelang, e bakang lefu la batho haholo-holo bakeng sa likokoana-hloko tsa ntaramane A le B, ke mofuta o le mong, kokoana-hloko ea RNA e arohaneng.Kokoana-hloko ea Influenza A ke ts'oaetso e matla ea ho hema, e kenyeletsang H1N1, H3N2 le mefuta e meng e nyenyane, e atisang ho fetoha le ho qhoma lefatšeng ka bophara."Shift" e bolela phetoho ea kokoana-hloko ea ntaramane A, e hlahisang ho hlaha ha kokoana-hloko e ncha "subtype".Livaerase tsa mokakallane oa B li arotsoe ka mela e 'meli, Yamagata le Victoria.Kokoana-hloko ea Influenza B e na le ho hoholeha ha antijeni, 'me e qoba ho lekoa le ho felisoa ha sesole sa 'mele oa motho ka phetoho ea eona.Leha ho le joalo, lebelo la ho iphetola ha lintho la kokoana-hloko ea ntaramane ea B le butle ho feta ba kokoana-hloko ea ntaramane A.Kokoana-hloko ea Influenza B e ka boela ea baka mafu a matšoafo a batho 'me ea baka mafu a seoa.

Adenovirus (AdV) ke ea mammalian adenovirus, e leng kokoana-hloko ea DNA e mahlakore a mabeli ntle le enfelopo.Bonyane ho fumanoe liphatsa tsa lefutso tse 90, tse ka aroloang ka AG 7 subgenera.Ts'oaetso ea AdV e ka baka mafu a fapaneng, ho kenyelletsa pneumonia, bronchitis, cystitis, conjunctivitis ea mahlo, mafu a mala le encephalitis.Adenovirus pneumonia ke e 'ngoe ea mefuta e matla haholo ea pneumonia e fumanoang ke sechaba ho bana, e etsang hoo e ka bang 4% -10% ea pneumonia e fumanoang ke sechaba.

Mycoplasma pneumoniae (MP) ke mofuta oa kokoana-hloko e nyenyane ka ho fetisisa ea prokaryotic, e pakeng tsa baktheria le kokoana-hloko, e nang le sebopeho sa lisele empa e se na lerako la sele.MP haholo e baka tšoaetso ea phefumoloho ea batho, haholo-holo ho bana le bacha.E ka baka nyumonia ea mycoplasma ea batho, tšoaetso ea matšoafo ea bana le pneumonia ea atypical.Lipontšo tsa kliniki li fapane, boholo ba tsona ke khohlela e matla, feberu, serame, hlooho e bohloko, 'metso.Tšoaetso e ka holimo ea matšoafo le pneumonia ea bronchial ke tsona tse atileng haholo.Bakuli ba bang ba ka hlaha ho tloha ho ts'oaetso e phahameng ea phefumoloho ho isa ho pneumonia e matla, khatello e matla ea ho hema le lefu le ka hlaha.

Respiratory syncytial virus (RSV) ke kokoana-hloko ea RNA, ea lelapa la paramyxoviridae.E fetisoa ke marotholi a moea le ho kopana haufi-ufi 'me ke eona kokoana-hloko e ka sehloohong ea tšoaetso e tlaase ea matšoafo ho masea.Masea a tšoaelitsoeng ke RSV a ka ’na a ba le bronchiolitis e matla (e bitsoang bronchiolitis) le pneumonia, tse amanang le asma ho bana.Masea a na le matšoao a matla, ho kenyelletsa feberu e phahameng, rhinitis, pharyngitis le laryngitis, ebe joale la bronchiolitis le pneumonia.Bana ba seng bakae ba kulang ba ka ba thata le otitis media, pleurisy le myocarditis, joalo-joalo Tšoaetso ea phefumoloho e ka holimo ke letšoao le ka sehloohong la tšoaetso ho batho ba baholo le bana ba baholo.

Channel

Lebitso la kanale	R6 Rection Buffer A	R6 Reaction Buffer B
FAM	SARS-CoV-2	HADV
VIC/HEX	Taolo ea ka hare	Taolo ea ka hare
CY5	IFV A	MP
ROX	IFV B	RSV

Tekheniki Parameters

Polokelo	Mokelikeli: ≤-18℃ Lefifing;Lyophilized: ≤30℃ Lefifing
Shelf-bophelo	Mokelikeli: likhoeli tse 9;Lyophilized: likhoeli tse 12
Mofuta oa Mohlala	Mali a felletseng, Plasma, Serum
Ct	≤38
CV	≤5.0％
LoD	300Likopi / mL
Tse khethehileng	Liphetho tsa li-cross-reactivity li bonts'itse hore ha ho na karabelo lipakeng tsa kit le coronavirus ea motho SARSr-CoV, MERSr-CoV, HCoV-OC43, HCoV-229E, HCoV-HKU1, HCoV-NL63, mofuta oa 1, 2, 3, rhinovirus A, B, C, chlamydia pneumoniae, metapneumovirus ea motho, enterovirus A, B, C, D, kokoana-hloko ea matšoafo ea motho, kokoana-hloko ea epstein-barr, kokoana-hloko ea maselese, kokoana-hloko ea cytomegalo, rotavirus, norovirus, kokoana-hloko ea parotitis, varicella-zoster virus, legionella, bordetella pertussis, haemophilus influenzae, staphylococcus aureus, streptococcus pneumoniae, s.pyogenes, klebsiella pneumoniae, lefuba la mycobacterium, aspergillus ea mosi, candida albicans, candida glabrata, pneumocystis jiroveci le cryptococcus e sa tsoa tsoaloa le acid ea motho ea genomic nucleic.
Lisebelisoa tse sebetsang	E ka tsamaisana le lisebelisoa tsa PCR tsa fluorescent tse fumanehang 'marakengLitsamaiso tsa PCR tsa nako ea sebele tsa SLAN-96P ABI 7500 Real-Nako PCR Systems ABI 7500 Fast Real-Nako PCR Systems QuantStudio^®Litsamaiso tse 5 tsa nako ea 'nete tsa PCR LightCycler^®Litsamaiso tsa PCR tsa Nako ea 480 LineGene 9600 Plus Nako ea 'Nete ea PCR ea ho Khetholla Sistimi MA-6000 Real-Nako Quantitative Thermal Cycler Sistimi ea PCR ea Nako ea 'Nete ea BioRad CFX96, BioRad Sistimi ea CFX Opus 96 ea Nako ea 'Nete ea PCR