Likokoana-hloko tsa ho hema tse kopantsoeng
Lebitso la sehlahisoa
Sete e Kopaneng ea ho Fumana Likokoana-hloko tsa ho Phefumoloha ea HWTS-RT183 (Fluorescence PCR)
Thuto ea mafu a seoa
Lefu la Vaerase ea Corona la 2019, le bitsoang 'COVID-19', le bolela pneumonia e bakoang ke tšoaetso ea SARS-CoV-2. SARS-CoV-2 ke coronavirus ea mofuta oa β. COVID-19 ke lefu le tšoaetsanoang le matla la ho hema, 'me baahi ba bangata ba kotsing. Hona joale, mohloli oa tšoaetso ke bakuli ba tšoaelitsoeng ke 2019-nCoV, 'me batho ba tšoaelitsoeng ba se nang matšoao le bona e ka ba mohloli oa tšoaetso. Ho latela lipatlisiso tsa hona joale tsa epidemiological, nako ea ho qala ke matsatsi a 1-14, boholo ba matsatsi a 3-7. Feberu, khohlela e omileng le mokhathala ke matšoao a mantlha. Bakuli ba 'maloa ba ne ba e-na le matšoao a kang ho thibana ha nko, nko e phallang, 'metso o bohloko, myalgia le letšollo, jj. Influenza, e tsejoang ka hore ke 'flu', ke lefu le matla la ho hema le bakoang ke kokoana-hloko ea ntaramane. Le tšoaetsanoa haholo. Le fetisoa haholo ka ho khohlela le ho thimola. Hangata le hlaha nakong ea selemo le mariha. Livaerase tsa ntaramane li arotsoe ka ntaramane A (IFV A), ntaramane B (IFV B), le ntaramane C (IFV C) mefuta e meraro, kaofela ke tsa kokoana-hloko e khomarelang, e bakang lefu la batho haholo-holo bakeng sa livaerase tsa ntaramane A le B, ke kokoana-hloko ea RNA e arohaneng ka thapo e le 'ngoe. Kokwana-hloko ea ntaramane A ke tšoaetso e matla ea ho hema, ho kenyeletsoa H1N1, H3N2 le mefuta e meng ea tsona, e kotsing ea ho fetoha le ho qhoma lefatšeng ka bophara. 'Shift' e bolela phetoho ea kokoana-hloko ea ntaramane A, e fellang ka ho hlaha ha 'subtype' e ncha ea kokoana-hloko. Livaerase tsa ntaramane B li arotsoe ka mela e 'meli, kokoana-hloko ea Yamagata le Victoria Influenza B e na le ho kheloha ha antigen feela, 'me e qoba tlhokomelo le ho felisoa ha sesole sa 'mele oa motho ka phetoho ea eona. Leha ho le joalo, lebelo la ho iphetola ha kokoana-hloko ea ntaramane B le lieha ho feta la kokoana-hloko ea ntaramane A ea motho. Kokwana-hloko ea ntaramane B e ka boela ea baka tšoaetso ea ho hema ea batho 'me ea lebisa mafung a seoa.
Vaerase ea syncytial ea phefumoloho (RSV) ke vaerase ea RNA, e tsoang lelapeng la paramyxoviridae. E fetisoa ka marotholi a moea le ho kopana haufi 'me ke eona kokoana-hloko e ka sehloohong ea tšoaetso ea methapo ea phefumoloho e tlase ho masea. Masea a tšoaelitsoeng ke RSV a ka ba le bronchiolitis e matla le pneumonia, tse amanang le asthma ho bana. Masea a na le matšoao a matla, ho kenyeletsoa feberu e phahameng, rhinitis, pharyngitis le laryngitis, ebe bronchiolitis le pneumonia. Bana ba 'maloa ba kulang ba ka rarahana le otitis media, pleurisy le myocarditis, jj. Tšoaetso ea methapo ea phefumoloho e ka holimo ke letšoao le ka sehloohong la tšoaetso ho batho ba baholo le bana ba baholo.
Li-parameter tsa Tekheniki
| Polokelo | -18℃ Lefifing |
| Nako ea ho phela | Likhoeli tse 9 |
| Mofuta oa Sampole | Swab ea molomo; Swab ea molomo |
| Ct | IFV A, IFVB, RSV, SARS-CoV-2, IFV A H1N1Ct≤38 |
| CV | ≤5% |
| LoD | Likopi tse 200/mL |
| Ho hlaka | Liphetho tsa ho kopana ha lisele li bontša hore ha ho na karabelo e kopaneng pakeng tsa kit le cytomegalovirus, mofuta oa 1 oa vaerase ea herpes simplex, vaerase ea varicella zoster, vaerase ea Epstein-Barr, adenovirus, human metapneumovirus, rhinovirus, parainfluenza virus type I/II/III/IV, bocavirus, enterovirus, coronavirus, Mycoplasma pneumoniae, Chlamydia pneumoniae, Bordetella pertussis, Corynebacterium spp., Escherichia coli, Haemophilus influenzae, Lactobacillus spp., Legionella pneumophila, Moraxella catarrhalis, mefuta e fokolitsoeng ea Mycobacterium tuberculosis, Neisseria meningitidis, Neisseria spp., Pseudomonas aeruginosa, Staphylococcus aureus, Streptococcus pneumoniae, Staphylococcus epidermidis, Streptococcus pyogenes, Streptococcus salivarius, Acinetobacter baumannii, Stenotrophomonas maltophilia, Burkholderia cepacia, Corynebacterium fasciatum, Nocardia, Serratia marcescens, Citrobacter rodentium, Cryptococcus, Aspergillus fumigatus, Aspergillus flavus, Pneumocystis carinii, Candida albicans, Roseburia mucosa, Streptococcus oralis, Klebsiella pneumoniae, Chlamydia psittaci, Rickettsia Q fever le human genomic nucleic acid. |
| Lisebelisoa tse Sebetsang | Mekhoa ea Li-biosystem tse Sebelisitsoeng 7500 Mekhoa ea PCR ea Nako ea 'Nete, Mekhoa ea Li-biosystem tse Sebelisitsoeng 7500 Mekhoa ea PCR e Potlakileng ea Nako ea 'Nete, QuantStudio®Mekhoa e 5 ea PCR ea Nako ea 'Nete, Mekhoa ea PCR ea Nako ea 'Nete ea SLAN-96P (Hongshi Medical Technology Co., Ltd.), LightCycler®Sistimi ea PCR ea Nako ea 'Nete ea 480, LineGene 9600 Plus Sistimi ea ho Fumana PCR ea Nako ea 'Nete (FQD-96A, theknoloji ea Hangzhou Bioer), MA-6000 Sesebelisoa sa ho Fuputsa Thermal sa Nako ea 'Nete sa MA-6000 (Suzhou Molarray Co., Ltd.), Sistimi ea PCR ea Nako ea 'Nete ea BioRad CFX96, Sistimi ea PCR ea Nako ea 'Nete ea BioRad CFX Opus 96. |
Phallo ea Mosebetsi
Sete ea Macro & Micro-Test Viral DNA/RNA (HWTS-3017) (e ka sebelisoang le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)), le Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-8) (e ka sebelisoang le EudemonTM AIO800 (HWTS-EQ007)) ke Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.
Bophahamo ba sampole e ntšitsoeng ke 200μL mme bophahamo bo kgothaletswang ba elution ke 150μL.
