Likokoana-hloko tse Phefumolohang li Kopanetsoe
Lebitso la sehlahisoa
HWTS-RT183-Likokoana-hloko tsa Phefumoloho tse Kopantsoeng tsa Temoho(Fluorescence PCR)
Epidemiology
Lefu la Corona Virus 2019, le bitsoang 'COVID-19', le bolela pneumonia e bakoang ke tšoaetso ea SARS-CoV-2. SARS-CoV-2 ke coronavirus ea mofuta oa β. COVID-19 ke lefu le tšoaetsanoang le matla la phefumoloho, 'me baahi ba bangata hangata ba kotsing. Hajoale, mohloli oa tšoaetso ke bakuli ba tšoaelitsoeng haholo ke 2019-nCoV, mme batho ba tšoaelitsoeng ba asymptomatic le bona e ka ba mohloli oa ts'oaetso. Ho ipapisitsoe le lipatlisiso tsa morao-rao tsa epidemiological, nako ea ho incubation ke matsatsi a 1-14, haholo matsatsi a 3-7. Feberu, ho khohlela ho omileng le mokhathala ke lipontšo tse ka sehloohong. Bakuli ba 'maloa ba ne ba e-na le matšoao a kang tšubuhlellano ea linko, nko e phallang,'metso, myalgia le letšollo, joalo-joalo. Influenza, e tsejoang ka hore ke 'ntaramane', ke lefu le tšoaetsanoang le matla la ho hema le bakoang ke kokoana-hloko ea ntaramane. E tšoaetsanoa haholo. E fetisoa haholo ke ho khohlela le ho thimola. Hangata e hlaha nakong ea selemo le mariha. Likokoana-hloko tsa ntaramane li arotsoe ka ntaramane A (IFV A), ntaramane B (IFV B), le Influenza C (IFV C) mefuta e meraro, kaofela ke ea kokoana-hloko e khomarelang, e bakang lefu la batho haholo-holo bakeng sa likokoana-hloko tsa ntaramane A le B, ke kokoana-hloko e le 'ngoe, e arohaneng ea RNA. Kokoana-hloko ea Influenza A ke ts'oaetso e matla ea ho hema, e kenyeletsang H1N1, H3N2 le mefuta e meng e nyenyane, e atisang ho fetoha le ho qhoma lefatšeng ka bophara. 'Shift' e bolela phetoho ea kokoana-hloko ea ntaramane A, e bakang ho hlaha ha kokoana-hloko e ncha 'subtype'. Livaerase tsa mokakallane oa B li arotsoe ka meloko e 'meli, kokoana-hloko ea Yamagata le Victoria Influenza B e na le antigeniki feela, 'me e qoba ho lebela le ho felisoa ha liente tsa motho ka phetoho ea eona. Leha ho le joalo, lebelo la ho iphetola ha lintho la kokoana-hloko ea ntaramane ea B le butle ho feta ba kokoana-hloko ea ntaramane A. Kokoana-hloko ea Influenza B e ka boela ea baka mafu a matšoafo a batho 'me ea baka mafu a seoa.
Respiratory syncytial virus (RSV) ke kokoana-hloko ea RNA, ea lelapa la paramyxoviridae. E fetisoa ka marotholi a moea le ho kopana haufi-ufi 'me ke eona kokoana-hloko e ka sehloohong ea tšoaetso e tlaase ea matšoafo ho masea. Masea a tšoaelitsoeng ke RSV a ka ’na a ba le bronchiolitis e matla le pneumonia, tse amanang le asma ho bana. Masea a na le matšoao a matla, ho kenyelletsa feberu e phahameng, rhinitis, pharyngitis le laryngitis, ebe joale la bronchiolitis le pneumonia. Bana ba seng bakae ba kulang ba ka ba thata ho otitis media, pleurisy le myocarditis, joalo-joalo.Tshoaetso ea phefumoloho e ka holimo ke letšoao le ka sehloohong la tšoaetso ho batho ba baholo le bana ba baholo.
Tekheniki Parameters
| Polokelo | -18℃ Lefifing |
| Shelf-bophelo | Likhoeli tse 9 |
| Mofuta oa Mohlala | lesela la Oropharyngeal; Nasopharyngeal swab |
| Ct | IFV A, IFVB, RSV, SARS-CoV-2, IFV A H1N1Ct≤38 |
| CV | ≤5% |
| LoD | 200 Likopi / μL |
| Tse khethehileng | Liphetho tsa cross-reactivity li bonts'a hore ha ho na karabelo e fapaneng lipakeng tsa kit le cytomegalovirus, herpes simplex virus type 1, varicella zoster virus, Epstein-Barr virus, adenovirus, human metapneumovirus, rhinovirus, parainfluenza virus type I/II/III/IV, bocavirus, enterovirus, coronavirus, Mycoplasmet pneumonia, Chlamydia Corynebacterium spp., Escherichia coli, Haemophilus influenzae, Lactobacillus spp., Legionella pneumophila, Moraxella catarrhalis, mefuta e fokolisitsoeng ea lefuba la Mycobacterium, Neisseria meningitidis, Neisseria spp. Streptococcus pneumoniae, Staphylococcus epidermidis, Streptococcus pyogenes, Streptococcus salivarius, Acinetobacter baumannii, Stenotrophomonas maltophilia, Burkholderia cepacia, Corynebacterium fasciatum, Nocardia, Serraticternscocus martopace, Cryptococter, Serraticternscocus martophilia, Aspergillus fumigatus, Aspergillus flavus, Pneumocystis carinii, Candida albicans, Roseburia mucosa, Streptococcus oalis, Klebsiella pneumoniae, Chlamydia psittaci, Rickettsia Q fever le human genomic nucleic acid. |
| Lisebelisoa tse sebetsang | Applied Biosystems 7500 Real-Time PCR Systems, Applied Biosystems 7500 Fast Real-Time PCR Systems, QuantStudio®Li-PCR Systems tse 5 tsa Nako ea 'Nete, SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.), LightCycler®480 Real-Time PCR system, LineGene 9600 Plus Real-Time PCR Detection Systems (FQD-96A, Hangzhou Bioer technology), MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.), BioRad CFX96 Real-Time PCR System, BioRad Real-Time PCR System Opus PCR 9 |
Phallo ea Mosebetsi
Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (e ka sebelisoang ka Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)), le Macro & Micro-Test Viral DNA/RNA Kit (HWTS-80) e sebelisa EWTS-30TM AIO800 (HWTS-EQ007)) ka Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.
Bolumo ea sampole e ntšitsoeng ke 200μL mme bophahamo ba molumo o khothaletsoang ke 150μL.
