Likokoana-hloko tse Phefumolohang li Kopanetsoe
Lintlha tse Kopantsoeng tsa Likokoana-hloko tsa ho Phefumoloha:
Lebitso la sehlahisoa
HWTS-RT050-Mefuta e Tšeletseng ea Phefumoloho ea Pathogen Nucleic Acid Detection Kit(Fluorescence PCR)
Epidemiology
Influenza, eo ka tlwaelo e tsejoang e le 'ntaramane', ke lefu le tšoaetsanoang le matla la ho hema le bakoang ke kokoana-hloko ea ntaramane, e tšoaetsanoang haholo 'me e fetisoa haholo ke ho khohlela le ho thimola.
Respiratory syncytial virus (RSV) ke kokoana-hloko ea RNA, ea lelapa la paramyxoviridae.
Human adenovirus (HAdV) ke kokoana-hloko ea DNA e likhoele habeli ntle le enfelopo. Bonyane 90 genotypes e fumanoe, e ka aroloa ka 7 subgenera AG.
Human rhinovirus (HRV) ke setho sa lelapa la Picornaviridae le mofuta oa Enterovirus.
Mycoplasma pneumoniae (MP) ke kokoana-hloko ea pathogenic e pakeng tsa baktheria le likokoana-hloko ka boholo.
Channel
| Channel | PCR-Mix A | PCR-Mix B |
| Mokhatlo oa FAM | IFV A | HADV |
| VIC/HEX Channel | HRV | IFV B |
| CY5 Channel | RSV | MP |
| ROX Channel | Taolo ea ka hare | Taolo ea ka hare |
Tekheniki Parameters
| Polokelo | -18℃ |
| Shelf-bophelo | Likhoeli tse 12 |
| Mofuta oa Mohlala | Oropharyngeal swab |
| Ct | ≤35 |
| LoD | 500Likopi / mL |
| Tse khethehileng | 1.Liphetho tsa liteko tsa cross-reactivity li bonts'itse hore ho ne ho se na karabelo e fapaneng lipakeng tsa kit le coronavirus ea motho SARSr-CoV, MERSr-CoV, HCoV-OC43, HCoV-229E, HCoV-HKU1, HCoV-NL63, mefuta ea vaerase ea Parainfluenza 1, 2, le 3, Chlamydia pneumoniaeumoviruse, Chlamydia pneumoniaeumovirus, B, Enapteroneviruse, B, Chlamydia pneumoniaeumoviruse, Chlamydia pneumoniaeumoviruse, B, Enapteroneviruse Kokoana ea Epstein-Barr, kokoana-hloko ea 'maselese, cytomegalovirus ea motho, Rotavirus, Norovirus, kokoana-hloko ea Mumps, Varicella-zoster virus, Legionella, Bordetella pertussis, Haemophilus influenzae, Staphylococcus aureus, Streptococcus pneumoniae, Streptococcus pyogenesis, Ksperllus pyogenesis, Ksperllus pyogenesis, Ksperllus pyogenesis fumigatus, Candida albicans, Candida glabrata, Pneumocystis jiroveci, Cryptococcus neoformans le human genomic nucleic acids. 2.Bokhoni ba ho thibela ho kena-kenana: Mucin (60mg/mL), 10% (v/v) mali a motho, phenylephrine (2mg/mL), oxymetazoline (2mg/mL), sodium chloride (e nang le li-preservatives) (20mg/mL), beclomethasone (20mg/mL), dexamethasone/0mL) triamcinolone acetonide (2mg/mL), budesonide (2mg/mL), mometasone (2mg/mL), Fluticasone (2mg/mL), histamine hydrochloride (5mg/mL), alpha-interferon (800IU/mL), zanamivir (20mg/mL) rimg/mL), oseamivir (20mg/mL) (60ng/mL), peramivir (1mg/mL), lopinavir (500mg/mL), ritonavir (60mg/mL), mupirocin (20mg/mL), azithromycin (1mg/mL), cefprozil (40μg/mL), Meropenem (200mg/mL), levofloxamy, levofloxamy (0.6mg / mL) e khethiloe bakeng sa tlhahlobo ea ho kena-kenana, 'me liphello li bontšitse hore lintho tse sitisang ho tse ka holimo li ne li se na karabelo ea ho kena-kenana le liphello tsa liteko tsa likokoana-hloko. |
| Lisebelisoa tse sebetsang | Applied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®Litsamaiso tse 5 tsa nako ea 'nete tsa PCR Litsamaiso tsa PCR tsa nako ea sebele tsa SLAN-96P LightCycler®Sistimi ea 480 ea Nako ea 'Nete ea PCR LineGene 9600 Plus Nako ea 'Nete ea PCR ea ho Khetholla Sistimi MA-6000 Real-Nako Quantitative Thermal Cycler BioRad CFX96 Real-Nako PCR System, BioRad CFX Opus 96 Real-Nako PCR System |
Kakaretso ea Tharollo ea PCR
Litšoantšo tsa lintlha tsa sehlahisoa:
Tataiso e Amanang ea Sehlahisoa:
Re lumela hore tšebelisano-'moho ea nako e telele e bakiloe ke maemo a holimo, mofani oa phaello, tsebo e atlehileng le ho ikopanya le motho ka mong bakeng sa Likokoana-hloko tse Phefumolohang, Sehlahisoa se tla fana ka lefats'e ka bophara, joalo ka: Casablanca, Montreal, Austria, re na le mohala o felletseng oa tlhahiso, tsamaiso ea taolo ea boleng, 'me ntho ea bohlokoa ka ho fetisisa, re na le lihlopha tse ngata tsa thekiso le litšebeletso tsa litsebi. Ka melemo eo kaofela, re tla theha lets'oao le tsebahalang la machaba la nylon monofilaments, le ho hasa lihlahisoa tsa rona likarolong tsohle tsa lefats'e. Re ntse re tsamaea 'me re leka ka hohle ho sebeletsa bareki ba rona.
Ka Alex ho tsoa Cancun - 2018.12.11 14:13 Fektheri e na le lisebelisoa tse tsoetseng pele, basebetsi ba nang le phihlelo le boemo bo botle ba tsamaiso, kahoo boleng ba sehlahisoa bo ne bo e-na le tiisetso, tšebelisano ena e phutholohile haholo ebile e thabile!
Ka Albert oa Philippines - 2017.05.02 11:33 Lihlopha tsa lihlahisoa
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