Mofuta oa kokoana-hloko ea polio Ⅰ
Lebitso la sehlahisoa
HWTS-EV006- Mofuta oa Poliovirus Ⅰ Nucleic Acid Detection Kit(Fluorescence PCR)
Epidemiology
Poliovirus ke kokoana-hloko e bakang poliomyelitis, lefu le tšoaetsanoang le tšoaetsanoang le atileng hohle.Hangata kokoana-hloko e hlasela tsamaiso ea methapo e bohareng, e senya lisele tsa methapo ea kutlo e lenakang le ka pele la lesapo la mokokotlo, 'me e baka ho holofala ha maoto le matsoho, e leng ntho e atileng haholo ho bana, kahoo e boetse e bitsoa polio.Likokoana-hloko tsa polio ke tsa mofuta oa enterovirus oa lelapa la picornaviridae.Kokoana-hloko ea polio e kena ’meleng oa motho ’me e nama haholo-holo ka tšilo ea lijo.E ka aroloa ka li-serotype tse tharo ho latela ho itšireletsa mafung, mofuta oa I, mofuta oa II, le mofuta oa III.
Channel
FAM | mofuta oa poliovirus I |
ROX | Taolo ea ka hare |
Tekheniki Parameters
Polokelo | ≤-18℃ |
Shelf-bophelo | Likhoeli tse 9 |
Mofuta oa Mohlala | Sampole ea mantle e sa tsoa bokelloa |
Ct | ≤38 |
CV | <5.0% |
LoD | 1000Likopi/mL |
Lisebelisoa tse sebetsang | Lisebelisoa tsa Biosystems 7500 tsa Nako ea 'Nete ea PCRApplied Biosystems 7500 Fast Real-Time PCR SystemsQuantStudio®Litsamaiso tse 5 tsa nako ea 'nete tsa PCRLitsamaiso tsa PCR tsa nako ea sebele tsa SLAN-96P LightCycler®Sistimi ea 480 ea Nako ea 'Nete ea PCR LineGene 9600 Plus Nako ea 'Nete ea PCR ea ho Fumana Sistimi MA-6000 Real-Nako Quantitative Thermal Cycler Sistimi ea PCR ea Nako ea 'Nete ea BioRad CFX96 BioRad CFX Opus 96 Real-Nako PCR System |
Phallo ea Mosebetsi
Khetho ea 1.
Li-reagents tse khothalelitsoeng: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (e ka sebelisoang le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B) by Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.. Molumo o lokela ho etsoa ho latela IFU ka tieo. Bolumo e khothaletsoang ea elution ke 80μL.
Khetho ea 2.
Li-reagents tse khothalelitsoeng: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3022) by Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.. Ho ntšoa ho lokela ho etsoa ho latela IFU ka tieo.Molumo o khothaletsoang oa elution ke 100μL.