Mofuta oa kokoana-hloko ea polio Ⅰ
Lebitso la sehlahisoa
HWTS-EV006- Sete ea ho lemoha asiti ea poliovirus ea mofuta oa Ⅰ (Fluorescence PCR)
Thuto ea mafu a seoa
Poliovirus ke vaerase e bakang poliomyelitis, lefu le tšoaetsanoang le matla le atang haholo. Hangata vaerase ena e hlasela tsamaiso ea methapo e bohareng, e senya lisele tsa methapo ea kutlo tse ka pele ho lenaka la mokokotlo, 'me e baka ho holofala ha maoto le matsoho, e leng ntho e atileng haholo ho bana, kahoo e boetse e bitsoa polio. Li-virus tsa polio ke tsa mofuta oa enterovirus oa lelapa la picornaviridae. Poliovirus e hlasela 'mele oa motho 'me e ata haholo-holo ka tsela ea tšilo ea lijo. E ka aroloa ka mefuta e meraro ea li-serotype ho latela tšireletso ea 'mele, mofuta oa I, mofuta oa II, le mofuta oa III.
Mokero
| FAM | kokoana-hloko ea polio ea mofuta oa I |
| ROX | Taolo ea Ka Hare |
Li-parameter tsa Tekheniki
| Polokelo | ≤-18℃ |
| Nako ea ho phela | Likhoeli tse 9 |
| Mofuta oa Sampole | Sampole ea mantle a sa tsoa bokelloa |
| Ct | ≤38 |
| CV | <5.0% |
| LoD | Likopi tse 1000/mL |
| Lisebelisoa tse Sebetsang | Sistimi ea PCR ea Nako ea 'Nete e Sebelisitsoeng ea 7500Mekhoa ea PCR e Potlakileng ea Li-Biosystem tse 7500 tsa Nako ea 'NeteQuantStudio®Mekhoa e 5 ea PCR ea Nako ea SebeleMekhoa ea PCR ea Nako ea Sebele ea SLAN-96P Leseli la ho Tsamaisa Li-Cycler®Sistimi ea PCR ea Nako ea 'Nete ea 480 Sistimi ea ho lemoha PCR ea LineGene 9600 Plus ea Nako ea 'Nete MA-6000 Nako ea Sebele ea Bongata ba Thermal Cycler Sistimi ea PCR ea BioRad CFX96 ea Nako ea 'Nete Sistimi ea PCR ea BioRad CFX Opus 96 ea Nako ea 'Nete |
Phallo ea Mosebetsi
Khetho ea 1.
Li-reagent tse khothaletsoang tsa ho ntša: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (e ka sebelisoang le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B) ke Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.. Ho ntša ho lokela ho etsoa ho latela IFU ka tieo. Bophahamo bo khothaletsoang ba ho tlosa ke 80μL.
Khetho ea 2.
Li-reagent tse khothaletsoang ho ntša: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3022) ea Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.. Ho ntša ho lokela ho etsoa ho latela IFU ka tieo. Bophahamo bo khothaletsoang ba ho tlosa ke 100μL.
