Chefo ea Clostridium difficile A/B gene (C.diff)
Lebitso la sehlahisoa
Sete ea ho Fumana Asiti ea Nyutlelie ea HWTS-OT031A bakeng sa chefo ea Clostridium difficile gene ea A/B (C.diff) (Fluorescence PCR)
Setifikeiti
CE
Thuto ea mafu a seoa
Clostridium difficile (CD), e leng gram-positive anaerobic sporogenic Clostridium difficile, ke e 'ngoe ea likokoana-hloko tse ka sehloohong tse bakang tšoaetso ea mala a nosocomial. Ho ea ka bongaka, hoo e ka bang 15% ~ 25% ea letšollo le amanang le likokoana-hloko, 50% ~ 75% ea colitis e amanang le likokoana-hloko le 95% ~ 100% ea pseudomembranous enteritis li bakoa ke tšoaetso ea Clostridium difficile (CDI). Clostridium difficile ke kokoana-hloko e nang le maemo, ho kenyeletsoa mefuta ea chefo le mefuta e seng chefo.
Mokero
| FAM | tcdAjini |
| ROX | tcdBjini |
| VIC/HEX | Taolo ea Ka Hare |
Li-parameter tsa Tekheniki
| Polokelo | ≤-18℃ |
| Nako ea ho phela | Likhoeli tse 12 |
| Mofuta oa Sampole | setuloana |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | 200CFU/mL |
| Ho hlaka | Sebelisa kit ena ho lemoha likokoana-hloko tse ling tsa mala tse kang Escherichia coli, Staphylococcus aureus, Shigella, Salmonella, Vibrio parahaemolyticus, Sehlopha sa B Streptococcus, Clostridium difficile mefuta e seng ea mafu, Adenovirus, rotavirus, norovirus, kokoana-hloko ea ntaramane A, kokoana-hloko ea ntaramane B le DNA ea genomic ea motho, liphello kaofela ha tsona li mpe. |
| Lisebelisoa tse Sebetsang | Mekhoa ea Li-biosystem tse Sebelisitsoeng 7500 Mekhoa ea PCR ea Nako ea Sebele Mekhoa ea PCR e Potlakileng ea Li-Biosystem tse 7500 tsa Nako ea 'Nete QuantStudio®Mekhoa e 5 ea PCR ea Nako ea Sebele Mekhoa ea PCR ea Nako ea 'Nete ea SLAN-96P (Hongshi Medical Technology Co., Ltd.) Leseli la ho Tsamaisa Li-Cycler®Sistimi ea PCR ea Nako ea 'Nete ea 480 Sistimi ea ho lemoha PCR ea LineGene 9600 Plus ea Nako ea 'Nete (FQD-96A，HangzhouTheknoloji ea Bioer) Sesebelisi sa Thermal sa MA-6000 sa Nako ea Sebele sa Bongata ba Thermal (Suzhou Molarray Co., Ltd.) Sistimi ea PCR ea BioRad CFX96 ea Nako ea 'Nete Sistimi ea PCR ea BioRad CFX Opus 96 ea Nako ea 'Nete |
Phallo ea Mosebetsi
Khetho ea 1.
Kenya 180μL ea lysozyme buffer ho precipitate (hlapolla lysozyme ho 20mg/mL ka lysozyme diluent), pipette ho kopanya hantle, 'me u e sebetse ho 37°C metsotso e fetang 30. Nka 1.5mL ea tube ea centrifuge e se nang RNase/DNase, 'me u e kenye.180μL ea taolo e ntle le taolo e mpe ka tatellano. Kenya10μL ea taolo ea ka hare ho sampole e lokelang ho lekoa, taolo e ntle, le taolo e mpe ka tatellano, 'me u sebelise Nucleic Acid Extraction kapa Purification Reagent (YDP302) ea Tiangen Biotech (Beijing) Co., Ltd. bakeng sa ho ntša DNA ea sampole e latelang, 'me ka kopo latela litaelo tsa tšebeliso ka tieo bakeng sa mehato e itseng. Sebelisa DNase/RNase free H2O bakeng sa tharollo, 'me bophahamo ba tharollo bo khothaletsoang ke 100μL.
Khetho ea 2.
Nka 1.5mL ea tube ea centrifuge e se nang RNase/DNase, 'me u kenye 200μL ea taolo e ntle le taolo e mpe ka tatellano.10μL ea taolo ea ka hare ho sampole e lokelang ho lekoa, taolo e ntle, le taolo e mpe ka tatellano, 'me u sebelise Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006). Ho ntšoa ho lokela ho etsoa ho latela litaelo tsa tšebeliso, 'me bophahamo bo khothaletsoang ba elution ke 80μL.
