Mumps Virus Nucleic Acid
Lebitso la sehlahisoa
HWTS-RT029-Mumps Virus Nucleic Acid Detection Kit(Fluorescence PCR)
Epidemiology
Kokoana-hloko ea 'mumps ke kokoana-hloko e le' ngoe ea serotype, empa mofuta oa protheine ea SH o fetoha haholo ho livaerase tse fapaneng tsa mumps. Kokoana-hloko ea Mumps e arotsoe ka li-genotypes tse 12 tse itšetlehileng ka phapang ea liphatsa tsa lefutso tsa SH, e leng mefuta ea A, B, C, D, F, G, H, I, J, K, L, le N. Kabo ea likokoana-hloko tsa genotypes ea mumps e na le litšobotsi tse hlakileng tsa libaka. Mefuta e atileng Europe haholo-holo ke liphatsa tsa lefutso A, C, D, G, le H; mefuta e meholo e atileng linaheng tsa Amerika ke mefuta ea C, D, G, H, J, le K; mefuta e ka sehloohong e atileng Asia ke liphatsa tsa lefutso B, F, I, le L; mofuta o ka sehloohong o atileng Chaena ke mofuta oa F; mefuta e atileng Japane le Korea Boroa ke mofuta oa B le 'na ka ho latellana. Ha ho hlake hore na mofuta ona oa kokoana-hloko e thehiloeng ho liphatsa tsa lefutso tsa SH o na le moelelo bakeng sa lipatlisiso tsa ente. Hajoale, mefuta ea ente e fokolisitsoeng e ntseng e sebelisoa lefatšeng ka bophara haholo-holo ke genotype A, 'me li-antibodies tse hlahisoang ke likokoana-hloko tsa likokoana-hloko tsa mefuta e fapaneng ea genotype li sireletsa.
Tekheniki Parameters
| Polokelo | -18℃ |
| Shelf-bophelo | Likhoeli tse 9 |
| Mofuta oa Mohlala | Ho phunya 'metso |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 1000 Likopi / mL |
| Lisebelisoa tse sebetsang | E sebetsa ho mochini oa ho lemoha oa mofuta oa I: Applied Biosystems 7500 Real-Nako PCR Systems, QuantStudio®Litsamaiso tse 5 tsa nako ea 'nete tsa PCR, SLAN-96P ea Nako ea Sebele PCR Systems (Hongshi Medical Technology Co., Ltd.), LineGene 9600 Plus Nako ea 'Nete ea PCR Detection Systems (FQD-96A, theknoloji ea Hangzhou Bioer), MA-6000 Real-Nako Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.), Sistimi ea PCR ea Nako ea 'Nete ea BioRad CFX96, BioRad CFX Opus 96 Real-Nako PCR System. E sebetsa ho mofuta oa II reagent ea ho lemoha: EudemonTMAIO800 (HWTS-EQ007) ka Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. |
Phallo ea Mosebetsi
Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (e ka sebelisoang ka Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)), le Macro & Micro-Test Viral DNA/RNA Kit (HWTS-80) e sebelisa EWTS-30TM AIO800 (HWTS-EQ007)) ka Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.
Bolumo ea sampole e ntšitsoeng ke 200μL mme bophahamo ba molumo o khothaletsoang ke 150μL.
