Monkeypox Virus Typing Nucleic Acid
Lebitso la sehlahisoa
HWTS-OT201Monkeypox Virus Typing Nucleic Acid Detection Kit(Fluorescence PCR)
Epidemiology
Monkeypox (Mpox) ke lefu le tšoaetsanoang le matla la zoonotic le bakoang ke Monkeypox Virus (MPXV). MPXV e na le sebopeho sa setene se chitja kapa se chitja, 'me ke kokoana-hloko ea DNA e nang le mela e 'meli e bolelele ba 197Kb. Boloetse bona bo fetisoa haholo ke liphoofolo, ’me batho ba ka tšoaetsoa ke ho longoa ke liphoofolo tse tšoaelitsoeng kapa ka ho kopana ka ho toba le mali, maro a ’mele le lekhopho la liphoofolo tse tšoaelitsoeng. Kokoana-hloko e ka boela ea fetisoa pakeng tsa batho, haholo-holo ka marotholi a ho hema nakong e telele, ka ho toba sefahleho le sefahleho kapa ka ho kopana ka ho toba le maro a 'mele oa mokuli kapa lintho tse nang le tšoaetso. Liphuputso li bontšitse hore MPXV e etsa lihlopha tse peli tse fapaneng: clade I (eo pele e neng e tsejoa e le Central African clade kapa Congo Basin clade) le clade II (eo pele e neng e bitsoa clade ea Afrika Bophirimela). Pox ya Congo Basin clade e bontshitswe ka ho hlaka hore e fetisetswa pakeng tsa batho mme e ka baka lefu, athe mpox ya clade ya Afrika Bophirima e baka matshwao a bobebe mme e na le sekhahla se tlase sa phetiso ho tloha ho motho ho ea ho motho.
Liphetho tsa tlhahlobo ea kit ena ha lia rereloa ho ba sesupo se le seng bakeng sa ho hlahlojoa ha tšoaetso ea MPXV ho bakuli, e lokelang ho kopanngoa le litšobotsi tsa mokuli tsa kliniki le lintlha tse ling tsa tlhahlobo ea laboratori ho ahlola ka nepo tšoaetso ea kokoana-hloko le ho etsa moralo o utloahalang oa phekolo ho netefatsa hore phekolo e sireletsehileng le e sebetsang.
Tekheniki Parameters
| Mofuta oa Mohlala | mokelikeli oa lekhopho la motho, oropharyngeal swab le serum |
| Ct | 38 |
| FAM | FAM-MPXV clade II VIC/HEX-MPXV clade I |
| CV | ≤5.0% |
| LoD | 200 Likopi / μL |
| Tse khethehileng | Sebelisa kit ho bona livaerase tse ling, joalo ka kokoana-hloko ea Sekholopane, kokoana-hloko ea Cowpox, Vaccinia virus, HSV1, HSV2, Human Herpesvirus mofuta 6, Human Herpesvirus mofuta 7, Human Herpesvirus type 8, Measels vieus, Chicken pox-Herpes zoster virus, EB virus, Rubella virus joalo-joalo, le ha ho na cross reaction. |
| Lisebelisoa tse sebetsang | Applied Biosystems 7500 Real-Time PCR Systems QuantStudio®Litsamaiso tse 5 tsa nako ea 'nete tsa PCR Litsamaiso tsa PCR tsa nako ea sebele tsa SLAN-96P LineGene 9600 Plus Nako ea 'Nete ea PCR ea ho Khetholla Sistimi MA-6000 Real-Nako Quantitative Thermal Cycler BioRad CFX96 Real-Nako PCR Systems BioRad CFX Opus 96 Real-Nako PCR Systems |
Phallo ea Mosebetsi
