Mofuta oa Kokwana-hloko ea Monkeypox Nucleic Acid
Lebitso la sehlahisoa
HWTS-OT201Sete ea ho Fumana Sekoahelo sa Kokwana-hloko ea Monkeypox ea Nucleic Acid(Phatsimo ea PCR ea Fluorescence)
Thuto ea mafu a seoa
Monkeypox (Mpox) ke lefu le tšoaetsanoang le bakoang ke Monkeypox Virus (MPXV). MPXV e na le sebopeho se chitja kapa se oval, 'me ke vaerase ea DNA e nang le mela e 'meli e bolelele ba hoo e ka bang 197Kb. Lefu lena le fetisoa haholo ke liphoofolo, 'me batho ba ka tšoaetsoa ka ho longoa ke liphoofolo tse tšoaelitsoeng kapa ka ho kopana ka ho toba le mali, maro a 'mele le makhopho a liphoofolo tse tšoaelitsoeng. Vaerase ena e ka boela ea fetisoa pakeng tsa batho, haholo-holo ka marotholi a ho hema nakong ea ho kopana ka nako e telele, ka ho toba le sefahleho kapa ka ho kopana ka ho toba le maro a 'mele oa mokuli kapa lintho tse silafetseng. Lithuto li bontšitse hore MPXV e theha lihlopha tse peli tse fapaneng: clade I (eo pele e neng e tsejoa e le clade ea Afrika Bohareng kapa clade ea Congo Basin) le clade II (eo pele e neng e bitsoa clade ea Afrika Bophirimela). Pox ea Congo Basin clade e bontšitsoe ka ho hlaka hore e ka fetisetsoa pakeng tsa batho 'me e ka baka lefu, ha mpox ea clade ea Afrika Bophirimela e baka matšoao a bobebe 'me e na le sekhahla se tlase sa phetisetso ho tloha ho motho ho ea ho motho.
Liphetho tsa liteko tsa kit ena ha lia rereloa ho ba sesupo feela sa ho fumanoa ha tšoaetso ea MPXV ho bakuli, e leng se lokelang ho kopanngoa le litšobotsi tsa bongaka tsa mokuli le lintlha tse ling tsa liteko tsa laboratori ho ahlola ka nepo tšoaetso ea pathogen le ho theha moralo o utloahalang oa kalafo ho netefatsa kalafo e sireletsehileng le e sebetsang.
Li-parameter tsa Tekheniki
| Mofuta oa Sampole | mokelikeli oa lekhopho la motho, swab ea oropharyngeal le serum |
| Ct | 38 |
| FAM | Sehlopha sa FAM-MPXV II Sehlopha sa VIC/HEX-MPXV I |
| CV | ≤5.0% |
| LoD | Likopi tse 200/μL |
| Ho hlaka | Sebelisa kit ho lemoha likokoana-hloko tse ling, tse kang kokoana-hloko ea Smallpox, kokoana-hloko ea Cowpox, kokoana-hloko ea Vaccinia, HSV1, HSV2, Herpesvirus ea Motho ea mofuta oa 6, Herpesvirus ea Motho ea mofuta oa 7, Herpesvirus ea Motho mofuta oa 8, Measels vieus, Chicken pox-Herpes zoster virus, EB virus, Rubella jj., le ha ho na karabelo e fapaneng. |
| Lisebelisoa tse Sebetsang | Mekhoa ea Li-biosystem tse Sebelisitsoeng 7500 Mekhoa ea PCR ea Nako ea Sebele QuantStudio®Mekhoa e 5 ea PCR ea Nako ea Sebele Mekhoa ea PCR ea Nako ea Sebele ea SLAN-96P Litsamaiso tsa ho lemoha PCR tsa LineGene 9600 Plus tsa Nako ea 'Nete MA-6000 Nako ea Sebele ea Bongata ba Thermal Cycler Mekhoa ea PCR ea BioRad CFX96 ea Nako ea 'Nete Mekhoa ea PCR ea BioRad CFX Opus 96 ea Nako ea 'Nete |
Phallo ea Mosebetsi
