Monkeypox Virus Nucleic Acid
Lebitso la sehlahisoa
HWTS-OT200 Monkeypox Virus Nucleic Acid Detection Kit(Enzymatic Probe Isothermal Amplification)
Setifikeiti
CE
Epidemiology
Monkeypox (MPX) ke lefu le tšoaetsanoang le matla la zoonotic le bakoang ke Monkeypox Virus (MPXV). MPXV e na le sebopeho sa setene se chitja kapa se chitja, 'me ke kokoana-hloko ea DNA e mahlakore a mabeli e bolelele ba 197Kb. Boloetse bona bo fetisoa haholo ke liphoofolo, ’me batho ba ka tšoaetsoa ke ho longoa ke liphoofolo tse tšoaelitsoeng kapa ka ho kopana ka ho toba le mali, maro a ’mele le lekhopho la liphoofolo tse tšoaelitsoeng. Kokoana-hloko e ka boela ea fetisoa pakeng tsa batho, haholo-holo ka marotholi a ho hema nakong e telele, ka ho toba sefahleho le sefahleho kapa ka ho kopana ka ho toba le maro a 'mele oa mokuli kapa lintho tse nang le tšoaetso. Matšoao a kliniki a tšoaetso ea monkeypox ho batho a tšoana le a sekholopane, hangata ka mor'a matsatsi a 12 a ho incubation, ho bonahala feberu, hlooho e bohloko, mesifa le mokokotlo, li-lymph nodes tse atolositsoeng, mokhathala le ho se phutholohe. Lekhopho le hlaha kamora matsatsi a 1-3 a feberu, hangata pele sefahlehong, empa le likarolong tse ling. Hangata lefu lena le nka libeke tse 2-4, 'me sekhahla sa lefu ke 1% -10%. Lymphadenopathy ke e 'ngoe ea liphapang tse kholo lipakeng tsa lefu lena le sekholopane.
Liphetho tsa liteko tsa kit ena ha lia lokela ho sebelisoa e le sesupo sa tlhahlobo ea tšoaetso ea kokoana-hloko ea monkeypox ho bakuli, e lokelang ho kopanngoa le litšoaneleho tsa kliniki tsa mokuli le lintlha tse ling tsa tlhahlobo ea laboratori ho fumana ka nepo tšoaetso ea kokoana-hloko, le etsa moralo o utloahalang oa phekolo ho etsa hore phekolo e bolokehe le ho sebetsa hantle.
Tekheniki Parameters
| Mofuta oa Mohlala | mokelikeli oa lekhopho la motho, oropharyngeal swab |
| Channel | FAM |
| Tt | 28 |
| CV | ≤5.0% |
| LoD | 200 Likopi / μL |
| Tse khethehileng | Sebelisa kit ho bona livaerase tse ling, joalo ka kokoana-hloko ea Sekholopane, kokoana-hloko ea Cowpox, Vaccinia virus,Herpes simplex kokoana-hloko, joalo-joalo, 'me ha ho na karabelo ea sefapano. |
| Lisebelisoa tse sebetsang | Easy Amp Real-time Fluorescence Isothermal Detection System (HWTS 1600) Applied Biosystems 7500 Real-Nako PCR Systems, QuantStudio®Litsamaiso tse 5 tsa nako ea 'nete tsa PCR Litsamaiso tsa PCR tsa nako ea 'nete tsa SLAN-96P LineGene 9600 Plus Nako ea 'Nete ea PCR ea ho Khetholla Sistimi MA-6000 Real-Nako Quantitative Thermal Cycler BioRad CFX96 PCR Systems ea Nako ea 'Nete, BioRad CFX Opus 96 Real-Nako PCR Systems. |
Phallo ea Mosebetsi
