Chlamydia Trachomatis e omisitsoeng ka leqhoa
Lebitso la sehlahisoa
HWTS-UR032C/D-Chlamydia Trachomatis e omisitsoeng ka leqhoa ea Nucleic Acid Detection Kit (Enzymatic Probe Isothermal Amplification)
Epidemiology
Chlamydia trachomatis (CT) ke mofuta oa likokoana-hloko tsa prokaryotic tse nang le likokoana-hloko ka ho feletseng liseleng tsa eukaryotic.[1].Chlamydia trachomatis e arotsoe ka AK serotypes ho ea ka serotype mokhoa.Urogenital tract infections hangata e bakoa ke trachoma biological variant DK serotypes, 'me banna ba atisa ho bonahatsoa e le urethritis, e ka imolloang ntle le phekolo, empa boholo ba bona bo fetoha bo sa foleng, nako le nako bo mpefala,' me bo ka kopanngoa le epididymitis, proctitis, joalo-joalo.[2].Basali ba ka bakoa ke urethritis, cervicitis, joalo-joalo, le mathata a tebileng a salpingitis.[3].
Channel
| FAM | Chlamydia trachomatis (CT) |
| ROX | Taolo ea ka hare |
Tekheniki Parameters
| Polokelo | ≤30 ℃ |
| Shelf-bophelo | Likhoeli tse 12 |
| Mofuta oa Mohlala | Sekoti sa molomo oa mosali Sekhahla sa botona Moroto o motona |
| Tt | ≤28 |
| CV | ≤10.0% |
| LoD | 400 Likopi / mL |
| Tse khethehileng | Ha ho na cross-reactivity lipakeng tsa kit ena le likokoana-hloko tse ling tsa tšoaetso ea genitourinary tract tse kang kotsi e kholo ea mofuta oa Human papillomavirus mofuta 16, Human papillomavirus mofuta 18, Herpes simplex virus mofuta Ⅱ, Treponema pallidum, Ureaplasma urealyticum, Mycoplasma hominis, Mycoplasma epilogenider , Escherichia coli, Gardnerella vaginalis, Candida albicans, Trichomonas vaginalis, Lactobacillus crispatus, Adenovirus, Cytomegalovirus, Beta Streptococcus, kokoana-hloko ea Human immunodeficiency virus, Lactobacillus casei le DNA ea motho ea genomic, joalo-joalo. |
| Lisebelisoa tse sebetsang | Lisebelisoa tsa Biosystems 7500 tsa Nako ea 'Nete ea PCR Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®Litsamaiso tse 5 tsa nako ea 'nete tsa PCR SLAN-96P ea Nako ea Sebele PCR Systems(Hongshi Medical Technology Co., Ltd.) LightCycler®Sistimi ea 480 ea Nako ea 'Nete ea PCR LineGene 9600 Plus Nako ea 'Nete ea PCR Detection System (FQD-96A, theknoloji ea Hangzhou Bioer) MA-6000 Real-Nako Quantitative Thermal Cycler BioRad CFX96 Real-Nako PCR System le BioRad CFX Opus 96 Real-Time PCR System Easy Amp Real-time Fluorescence Isothermal Detection System（HWTS-1600）. |
Phallo ea Mosebetsi
Khetho ea 1.
Macro & Micro-Test Sample Release Reagent (HWTS-3005-8).Ho ntšoa ho lokela ho etsoa ho latela melao ea IFU.Kenya sampole ea DNA e ntšitsoeng ke sampole ea tokollo ka har'a buffer ea karabelo 'me u hlahlobe sesebelisoa ka kotloloho, kapa lisampole tse ntšitsoeng li lokela ho bolokoa ho 2-8℃ nako e sa feteng lihora tse 24.
Khetho ea 2.
Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).Ho ntšoa ho lokela ho etsoa ho latela IFU, 'me bophahamo ba molumo o khothaletsoang ke 80μL.Mohlala oa DNA o ntšitsoeng ka mokhoa oa magnetic bead o futhumatsoa ho 95 ° C metsotso e 3 ebe hang-hang o hlatsoa leqhoa ka metsotso e 2.Kenya sampole e sebetsitsoeng ea DNA ka har'a buffer 'me u hlahlobe sesebelisoa kapa lisampole tse sebetsitsoeng li lokela ho bolokoa ka tlase ho -18°C nako e sa feteng likhoeli tse 4.Palo ea ho qhoqhoa khafetsa le ho qhibiliha ha ea lokela ho feta lipotoloho tse 4.
