Monkeypox Virus le Typing Nucleic Acid

Tlhaloso e Khutšoanyane:

Kit ena e sebelisetsoa ho lemoha boleng ba in vitro virus ea monkeypox clade I, clade II le monkeypox virus universal nucleic acids mokelikeli oa motho oa lekhopho, li-oropharyngeal swabs le disampole tsa serum.

Re romelle lengolo-tsoibila

Lintlha tsa Sehlahisoa

Li-tag tsa Sehlahisoa

Lebitso la sehlahisoa

HWTS-OT202-Monkeypox Virus le Typing Nucleic Acid Detection Kit (Fluorescence PCR)

Setifikeiti

Epidemiology

Monkeypox (Mpox) ke lefu le tšoaetsanoang le matla la zoonotic le bakoang ke Monkeypox Virus (MPXV). MPXV e na le sebopeho sa setene se chitja kapa se chitja, 'me ke kokoana-hloko ea DNA e mahlakore a mabeli e bolelele ba 197Kb.^[1]. Boloetse bona bo fetisoa haholo ke liphoofolo, ’me batho ba ka tšoaetsoa ke ho longoa ke liphoofolo tse tšoaelitsoeng kapa ka ho kopana ka ho toba le mali, maro a ’mele le lekhopho la liphoofolo tse tšoaelitsoeng. Kokoana-hloko e ka boela ea fetisoa pakeng tsa batho, haholo-holo ka marotholi a ho hema nakong e telele, ka ho toba sefahleho le sefahleho kapa ka ho kopana ka ho toba le maro a 'mele oa mokuli kapa lintho tse nang le tšoaetso.^[2-3]. Liphuputso li bontšitse hore MPXV e etsa lihlopha tse peli tse fapaneng: clade I (eo pele e neng e tsejoa e le Central African clade kapa Congo Basin clade) le clade II (eo pele e neng e bitsoa clade ea Afrika Bophirimela). Pox ya Congo Basin clade e bontshitswe ka ho hlaka hore e fetisetswa pakeng tsa batho mme e ka baka lefu, athe mpox ya clade ya Afrika Bophirima e baka matshwao a bobebe mme e na le sekhahla se tlase sa phetiso ho tloha ho motho ho ea ho motho.^[4].

Liphetho tsa tlhahlobo ea kit ena ha lia rereloa ho ba sesupo se le seng bakeng sa ho hlahlojoa ha tšoaetso ea MPXV ho bakuli, e lokelang ho kopanngoa le litšobotsi tsa mokuli tsa kliniki le lintlha tse ling tsa tlhahlobo ea laboratori ho ahlola ka nepo tšoaetso ea kokoana-hloko le ho etsa moralo o utloahalang oa phekolo ho netefatsa hore phekolo e sireletsehileng le e sebetsang.

Channel

FAM	MPXV sehlopha II
ROX	MPXV universal nucleic acid
VIC/HEX	Sehlopha sa MPXV I
CY5	taolo ya ka hare

Tekheniki Parameters

Polokelo	≤-18℃
Shelf-bophelo	Likhoeli tse 12
Mofuta oa Mohlala	mokelikeli oa lekhopho la motho, li-oropharyngeal swabs le serum
Ct	≤38 (FAM, VIC/HEX, ROX), ≤35（IC)
LoD	200 likopi / mL
Lisebelisoa tse sebetsang	Sesebelisoa sa ho lemoha mofuta oa I: Applied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio^®Litsamaiso tse 5 tsa nako ea 'nete tsa PCR SLAN-96P ea Nako ea Sebele PCR Systems (Hongshi Medical Technology Co., Ltd.) LightCycler^®Sistimi ea 480 ea Nako ea 'Nete ea PCR LineGene 9600 Plus Nako ea 'Nete ea PCR Detection Systems (FQD-96A, theknoloji ea Hangzhou Bioer) MA-6000 Nako ea 'Nete ea Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) Sistimi ea PCR ea Nako ea 'Nete ea BioRad CFX96 BioRad CFX Opus 96 Real-Nako PCR System Sehlahisoa sa ho fumana mofuta oa II: Eudemon^TMAIO800 (HWTS-EQ007)) ka Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.