Vaerase ea Monkeypox le Mofuta oa Nucleic Acid

Setšoantšo se Hlahisitsoeng sa Vaerase ea Monkeypox le Typing Nucleic Acid

Tlhaloso e Khutšoanyane:

Sete ena e sebelisetsoa ho lemoha ka hare ho vitro boleng ba monkeypox virus clade I, clade II le monkeypox virus universal nucleic acids mokelikeling oa rash oa motho, li-oropharyngeal swabs le lisampoleng tsa serum.

Romella imeile ho rona

Qaqiso ea Sehlahisoa

Li-tag tsa Sehlahisoa

Lebitso la sehlahisoa

Kiti ea ho lemoha kokoana-hloko ea HWTS-OT202-Monkeypox le Typeing Nucleic Acid Detection Kit (Fluorescence PCR)

Setifikeiti

Thuto ea mafu a seoa

Monkeypox (Mpox) ke lefu le tšoaetsanoang le bakoang ke Monkeypox Virus (MPXV). MPXV e na le sebopeho se chitja kapa se oval, 'me ke vaerase ea DNA e nang le mela e 'meli e bolelele ba hoo e ka bang 197Kb.^[1]. Lefu lena le fetisoa haholo-holo ke liphoofolo, 'me batho ba ka tšoaetsoa ke ho longoa ke liphoofolo tse tšoaelitsoeng kapa ka ho kopana ka ho toba le mali, maro a 'mele le makhopho a liphoofolo tse tšoaelitsoeng. Vaerase ena e ka boela ea fetisoa pakeng tsa batho, haholo-holo ka marotholi a ho hema nakong ea ho kopana ka nako e telele, ka ho toba le lifahleho kapa ka ho kopana ka ho toba le maro a 'mele oa mokuli kapa lintho tse silafetseng.^[2-3]. Lithuto li bontšitse hore MPXV e theha lihlopha tse peli tse fapaneng: clade I (eo pele e neng e tsejoa e le clade ea Afrika Bohareng kapa clade ea Congo Basin) le clade II (eo pele e neng e bitsoa clade ea Afrika Bophirimela). Mpox ea Congo Basin clade e bontšitsoe ka ho hlaka hore e ka fetisetsoa pakeng tsa batho 'me e ka baka lefu, ha mpox ea Afrika Bophirimela e baka matšoao a bobebe 'me e na le sekhahla se tlase sa phetisetso ho tloha ho motho ho ea ho motho.^[4].

Liphetho tsa liteko tsa kit ena ha lia rereloa ho ba sesupo feela sa ho fumanoa ha tšoaetso ea MPXV ho bakuli, e leng se lokelang ho kopanngoa le litšobotsi tsa bongaka tsa mokuli le lintlha tse ling tsa liteko tsa laboratori ho ahlola ka nepo tšoaetso ea pathogen le ho theha moralo o utloahalang oa kalafo ho netefatsa kalafo e sireletsehileng le e sebetsang.

Mokero

FAM	MPXV class II
ROX	Asiti ea nucleic ea bokahohle ea MPXV
VIC/HEX	Letlapa la MPXV I
CY5	taolo ea ka hare

Li-parameter tsa Tekheniki

Polokelo	≤-18℃
Nako ea ho phela	Likhoeli tse 12
Mofuta oa Sampole	mokelikeli oa lekhopho la motho, li-swab tsa oropharyngeal le serum
Ct	≤38 (FAM, VIC/HEX, ROX), ≤35 (IC)
LoD	Likopi tse 200/mL
Lisebelisoa tse Sebetsang	Sesebediswa sa ho lemoha sa Mofuta wa I: Mekhoa ea Li-biosystem tse Sebelisitsoeng 7500 Mekhoa ea PCR ea Nako ea Sebele Mekhoa ea PCR e Potlakileng ea Li-Biosystem tse 7500 tsa Nako ea 'Nete QuantStudio^®Mekhoa e 5 ea PCR ea Nako ea Sebele Mekhoa ea PCR ea Nako ea 'Nete ea SLAN-96P (Hongshi Medical Technology Co., Ltd.) Leseli la ho Tsamaisa Li-Cycler^®Sistimi ea PCR ea Nako ea 'Nete ea 480 LineGene 9600 Plus Mekhoa ea ho Lemoha PCR ea Nako ea 'Nete (FQD-96A, theknoloji ea Hangzhou Bioer) Sesebelisi sa Thermal sa MA-6000 sa Nako ea Sebele sa Bongata ba Thermal (Suzhou Molarray Co., Ltd.) Sistimi ea PCR ea BioRad CFX96 ea Nako ea 'Nete Sistimi ea PCR ea BioRad CFX Opus 96 ea Nako ea 'Nete Sesebelisi sa ho lemoha sa Mofuta oa II: Eudemon^TMAIO800 (HWTS-EQ007)) ke Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.