Middle East Respiratory Syndrome Coronavirus Nucleic Acid
Lebitso la sehlahisoa
HWTS-RT031A-Middle East Respiratory Syndrome Coronavirus Nucleic Acid Detection Kit(Fluorescent PCR)
Epidemiology
Middle East Respiratory Syndrome coronavirus (MERS-CoV), β-coronavirus e bakanglefu la phefumoloho ho batho, le ile la tsejoa ka lekhetlo la pele ho mokuli e motona ea lilemo li 60 ea shoeleng oa Saudi Arabia ka 24th July, 2012. Tlhahiso ea meriana ea tšoaetso ea MERS-CoV e tloha ho boemo bo sa tsitsang kapa matšoao a bonolo a ho hema ho ea ho lefu le matla la ho hema esita le lefu.
Channel
FAM | RNA kokoana-hloko ea MERS |
VIC(HEX) | Taolo ea ka hare |
Tekheniki Parameters
Polokelo | ≤-18℃ Lefifing |
Shelf-bophelo | Likhoeli tse 9 |
Mofuta oa Mohlala | Li-swabs tsa nasopharyngeal tse sa tsoa bokelloa |
CV | ≤5.0% |
Ct | ≤38 |
LoD | 1000 Likopi / mL |
Tse khethehileng | Ha ho na cross-reactivity with human coronaviruses human coronavirus SARSr-CoV le likokoana-hloko tse ling tse tloaelehileng. |
Lisebelisoa tse Sebetsang: | Applied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Nako ea 'Nete ea PCR Systems Litsamaiso tsa PCR tsa nako ea sebele tsa SLAN-96P Sesebelisoa sa PCR sa LightCycler®480 Real-Time LineGene 9600 Plus Nako ea 'Nete ea PCR ea ho Fumana Sistimi MA-6000 Real-Nako Quantitative Thermal Cycler Sistimi ea PCR ea Nako ea 'Nete ea BioRad CFX96 BioRad CFX Opus 96 Real-Nako PCR System |
Phallo ea Mosebetsi
Khetho ea 1.
Sehlahisoa se khothalelitsoeng: QIAamp Viral RNA Mini Kit (52904), Nucleic Acid Extraction or Purification Reagent (YDP315-R) by Tiangen Biotech (Beijing) Co., Ltd.
Khetho ea 2.
Sehlahisoa sa ho ntša se khothalelitsoeng: Macro & Micro-Test General DNA/RNA Kit (HWTS-3017) le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).