Lefu la ho hema la Bochabela bo Hare Coronavirus Nucleic Acid
Lebitso la sehlahisoa
HWTS-RT031A-Bochabela bo Hare ba ho Phefumoloha ha Coronavirus Nucleic Acid Detection Kit (Fluorescent PCR)
Thuto ea mafu a seoa
Bolwetse ba Coronavirus ba Bochabela bo Hare (MERS-CoV), vaerase ya β-coronavirus e bakanglefu la ho hema ho batho, le ile la fumanoa ka lekhetlo la pele ho mokuli oa monna oa Saudi Arabia ea lilemo li 60 ea hlokahetseng ka la 24 Phupu, 2012. Tlhahiso ea kliniki ea tšoaetso ea MERS-CoV e tloha boemong ba ho se be le matšoao kapa matšoao a bobebe a ho hema ho ea ho lefu le matla la ho hema esita le lefu.
Mokero
| FAM | RNA ea kokoana-hloko ea MERS |
| VIC(HEX) | Taolo ea Ka Hare |
Li-parameter tsa Tekheniki
| Polokelo | ≤-18℃ Lefifing |
| Nako ea ho phela | Likhoeli tse 9 |
| Mofuta oa Sampole | Li-swab tsa nasopharyngeal tse sa tsoa bokelloa |
| CV | ≤5.0% |
| Ct | ≤38 |
| LoD | Likopi tse 1000/mL |
| Ho hlaka | Ha ho na karabelo e ts'oanang le li-coronavirus tsa batho. Coronavirus ea batho SARSr-CoV le likokoana-hloko tse ling tse tloaelehileng. |
| Lisebelisoa tse Sebetsang: | Mekhoa ea Li-biosystem tse Sebelisitsoeng 7500 Mekhoa ea PCR ea Nako ea Sebele Mekhoa ea PCR e Potlakileng ea Li-Biosystem tse 7500 tsa Nako ea 'Nete Mekhoa ea PCR ea QuantStudio®5 ea Nako ea 'Nete Mekhoa ea PCR ea Nako ea Sebele ea SLAN-96P Sistimi ea PCR ea LightCycler®480 ea Nako ea 'Nete Sistimi ea ho lemoha PCR ea LineGene 9600 Plus ea Nako ea 'Nete MA-6000 Nako ea Sebele ea Bongata ba Thermal Cycler Sistimi ea PCR ea BioRad CFX96 ea Nako ea 'Nete Sistimi ea PCR ea BioRad CFX Opus 96 ea Nako ea 'Nete |
Phallo ea Mosebetsi
Khetho ea 1.
Reagent e kgothalletswang ya ho ntsha: QIAamp Viral RNA Mini Kit (52904), Nucleic Acid Extraction kapa Purification Reagent (YDP315-R) ke Tiangen Biotech (Beijing) Co., Ltd.
Khetho ea 2.
Reagent e kgothaletswang ya ho ntsha: Kit ya Macro & Micro-Test General DNA/RNA (HWTS-3017) le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).
Lihlopha tsa lihlahisoa
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Mefuta e tšeletseng ea likokoana-hloko tsa ho hema
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AdV Universal le Mofuta oa 41 Nucleic Acid
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Asiti ea Nucleic Acid ea Influenza A
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Antigen ea ntaramane A/B
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Mycoplasma Pneumoniae Nucleic Acid