Kokoana-hloko ea Influenza A / Influenza B Virus
Lebitso la sehlahisoa
HWTS-RT174-Influenza A Virus/ Influenza B Virus Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Ho ipapisitsoe le liphapang tsa antigenic lipakeng tsa NP gene le M gene, livaerase tsa ntaramane li ka aroloa ka mefuta e mene: kokoana-hloko ea ntaramane A (IFV A), kokoana-hloko ea ntaramane B (IFV B), kokoana-hloko ea ntaramane C (IFV C) le kokoana-hloko ea ntaramane D (IFV D)[1]. Kokoana-hloko ea Influenza A e na le likokoana-hloko tse ngata le li-serotypes tse rarahaneng, 'me e ka fumana bokhoni ba ho hasana hohle ka ho kopanya liphatsa tsa lefutso le liphetoho tse fetohang. Batho ha ba na tšireletso ea nako e telele ho kokoana-hloko ea ntaramane A, kahoo batho ba lilemo tsohle hangata ba hlaseleha habonolo. Kokoana-hloko ea Influenza A ke eona pathogen e ka sehloohong e bakang mafu a seoa[2]. Kokoana-hloko ea Influenza B e atile haholo sebakeng se senyenyane mme ha joale ha e na mefuta e nyane. Tse ka sehloohong tse bakang tshwaetso ya batho ke leloko la B/Yamagata kapa leloko la B/Victoria. Har'a linyeoe tse netefalitsoeng tsa ntaramane linaheng tse 15 sebakeng sa Asia-Pacific khoeli le khoeli, sekhahla se tiisitsoeng sa kokoana-hloko ea ntaramane ea B ke 0-92%[3]. Ho fapana le kokoana-hloko ea ntaramane A, lihlopha tse khethehileng tse kang bana le batho ba hōlileng li kotsing ea ho tšoaetsoa kokoana-hloko ea ntaramane ea B 'me li atisa ho ba le mathata, e leng se behang sechaba moroalo o moholo ho feta kokoana ea ntaramane A.[4].
Channel
| FAM | MP nucleic acid |
| ROX | Taolo ea ka hare |
Tekheniki Parameters
| Polokelo | ≤-18℃ |
| Shelf-bophelo | Likhoeli tse 12 |
| Mofuta oa Mohlala | Mohlala oa oropharyngeal swab |
| Ct | Mokhohlane A, Mokhohlane BCt≤35 |
| CV | <5.0% |
| LoD | Fluu A le Flu Bkaofela ke 200Copies/mL |
| Tse khethehileng | Cross-reactivity: Ha ho na karabelo pakeng tsa kit le Bocavirus, rhinovirus, cytomegalovirus, breath syncytial virus, parainfluenza virus, Epstein-Barr virus, herpes simplex virus, Varicella-zoster virus, mumps virus, enterovirus, maselese kokoana-hloko, human metapneumovirus, adenovirus, humanrovirus, SARS novel coronavirus, SARS novel coronavirus Chlamydia pneumoniae, Mycoplasma pneumoniae, Streptococcus pneumoniae, Klebsiella pneumoniae, Streptococcus pyogenes, Legionella, Pneumocystis carinii, Haemophilus influenzae, Bordetella pertussis, Staphylococcus aureus, Mycobacterium tuberculosis, Candida TB glabrata, Aspergillus fumigatus, Cryptococcus neoformans, Streptococcus salivarius, Moraxella catarrhalis, Lactobacillus, Corynebacterium, le DNA ea motho ea genomic. Teko ea tšitiso: Khetha mucin (60 mg/mL), mali a motho (50%), phenylephrine (2mg/mL), oxymetazoline (2mg/mL), sodium chloride (20mg/mL) e nang le 5% ea preservative, beclomethasone (20mg/mL), dexamethasone (20mg/mL), flunisognodem2 acetonide (2mg/mL), budesonide (1mg/mL), mometasone (2mg/mL), fluticasone (2mg/mL), histamine hydrochloride (5mg/mL), benzocaine (10%), menthol (10%), zanamivir (20mg/mL), peramivir (1mg/mL), peramivir (1mg/mL) (0.6mg/mL), oseltamivir (60ng/mL), ribavirin (10mg/L) bakeng sa liteko tsa ho kena-kenana le tšitiso, ’me liphetho li bontša hore lintho tse sitisang likhatisong tse ka holimo ha li sitise ho fumanoa ha khiti. |
| Lisebelisoa tse sebetsang | Litsamaiso tsa PCR tsa nako ea sebele tsa SLAN-96P Lisebelisoa tsa Biosystems 7500 tsa Nako ea 'Nete ea PCR QuantStudio®Litsamaiso tse 5 tsa nako ea 'nete tsa PCR LineGene 9600 Plus Nako ea 'Nete ea PCR ea ho Fumana Sistimi MA-6000 Real-Nako Quantitative Thermal Cycler Sistimi ea PCR ea Nako ea 'Nete ea BioRad CFX96 BioRad CFX Opus 96 Real-Nako PCR System |
Phallo ea Mosebetsi
Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (e ka sebelisoang le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) ka Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.ba kgothaletswa bakeng sa mohlala ntsa lemehato e latelang e lokela ho batsamaisated ka tieo ho latela IFUea Kit.
