Phetoho ea Liphatsa tsa Lefutso ea PML-RARA ea Motho
Lebitso la sehlahisoa
HWTS-TM017AKit ea ho Fumana Phetoho ea Liphatsa tsa Lefutso ea PML-RARA ea Motho (Fluorescence PCR)
Thuto ea mafu a seoa
Leukemia e matla ea promyelocytic (APL) ke mofuta o ikhethileng oa leukemia e matla ea myeloid (AML). Hoo e ka bang 95% ea bakuli ba APL ba tsamaea le phetoho e ikhethang ea cytogenetic, e leng t(15;17)(q22;q21), e etsang hore gene ea PML ho chromosome 15 le gene ea retinoic acid receptor α (RARA) ho chromosome 17 li kopane ho theha gene ea PML-RARA fusion. Ka lebaka la lintlha tse fapaneng tsa karohano ea gene ea PML, gene ea PML-RARA fusion e ka aroloa ka mofuta o molelele (mofuta oa L), mofuta o mokhutšoane (mofuta oa S) le mofuta o fapaneng (mofuta oa V), tse etsang hoo e ka bang 55%, 40% le 5% ka ho latellana.
Mokero
| FAM | Jini ea motsoako oa PML-RARA |
| ROX | Taolo ea Ka Hare |
Li-parameter tsa Tekheniki
| Polokelo | ≤-18℃ Lefifing |
| Nako ea ho phela | Likhoeli tse 9 |
| Mofuta oa Sampole | moko oa masapo |
| CV | <5.0% |
| LoD | Likopi tse 1000/mL. |
| Ho hlaka | Ha ho na phetoho e ts'oanang le liphatsa tse ling tsa lefutso la motsoako oa BCR-ABL, E2A-PBX1, MLL-AF4, AML1-ETO, le TEL-AML1. |
| Lisebelisoa tse Sebetsang | Sistimi ea PCR ea Nako ea 'Nete e Sebelisitsoeng ea 7500 Mekhoa ea PCR e Potlakileng ea Li-Biosystem tse 7500 tsa Nako ea 'Nete QuantStudio®Mekhoa e 5 ea PCR ea Nako ea Sebele Mekhoa ea PCR ea Nako ea 'Nete ea SLAN-96P (Hongshi Medical Technology Co., Ltd.) Leseli la ho Tsamaisa Li-Cycler®Sistimi ea PCR ea Nako ea 'Nete ea 480 Sistimi ea ho lemoha PCR ea LineGene 9600 Plus ea Nako ea 'Nete (FQD-96A, theknoloji ea Hangzhou Bioer) Sesebelisi sa Thermal sa MA-6000 sa Nako ea Sebele sa Bongata ba Thermal (Suzhou Molarray Co., Ltd.) Sistimi ea PCR ea BioRad CFX96 ea Nako ea 'Nete Sistimi ea PCR ea BioRad CFX Opus 96 ea Nako ea 'Nete |
Phallo ea Mosebetsi
Setlhare se kgothaletswang sa ho ntsha: RNAprep Pure Blood Total RNA Extraction Kit (DP433). Ho ntsha ho lokela ho etswa ho ya ka IFU.
