Motho PML-RARA Fusion Gene Phetoho

Motho PML-RARA Fusion Gene Mutation Featured Image

Tlhaloso e Khutšoanyane:

Setsi sena se sebelisetsoa ho lemoha boleng ba phatsa ea lefutso ea PML-RARA ka har'a lisampole tsa moko oa motho ka har'a vitro.

Re romelle lengolo-tsoibila

Lintlha tsa Sehlahisoa

Li-tag tsa Sehlahisoa

Lebitso la sehlahisoa

HWTS-TM017AHuman PML-RARA Fusion Gene Mutation Detection Kit (Fluorescence PCR)

Epidemiology

Acute promyelocytic leukemia (APL) ke mofuta o khethehileng oa acute myeloid leukemia (AML). Hoo e ka bang 95% ea bakuli ba APL ba tsamaisana le phetoho e khethehileng ea cytogenetic, e leng t (15; 17) (q22; q21), e etsang hore PML gene ka chromosome 15 le retinoic acid receptor α gene (RARA) ka chromosome 17 e kopantsoeng ho theha PML-RARA gene fusion. Ka lebaka la likarolo tse fapaneng tsa mofuta oa PML, mofuta oa PML-RARA oa fusion o ka aroloa ka mofuta o molelele (L mofuta), mofuta o mokhuts'oane (S mofuta) le mofuta o fapaneng (mofuta oa V), o nkang hoo e ka bang 55%, 40% le 5% ka ho latellana.

Channel

FAM	PML-RARA ea motsoako oa lefutso
ROX	Taolo ea ka hare

Tekheniki Parameters

Polokelo	≤-18℃ Lefifing
Shelf-bophelo	Likhoeli tse 9
Mofuta oa Mohlala	moko oa masapo
CV	<5.0％
LoD	1000 Likopi / mL.
Tse khethehileng	Ha ho na cross-reactivity le mefuta e meng ea fusion BCR-ABL, E2A-PBX1, MLL-AF4, AML1-ETO, le TEL-AML1 fusion ea liphatsa tsa lefutso.
Lisebelisoa tse sebetsang	Lisebelisoa tsa Biosystems 7500 tsa Nako ea 'Nete ea PCR Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio^®Litsamaiso tse 5 tsa nako ea 'nete tsa PCR SLAN-96P ea Nako ea Sebele PCR Systems (Hongshi Medical Technology Co., Ltd.) LightCycler^®Sistimi ea 480 ea Nako ea 'Nete ea PCR LineGene 9600 Plus Nako ea 'Nete ea PCR Detection System (FQD-96A, theknoloji ea Hangzhou Bioer) MA-6000 Nako ea 'Nete ea Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) Sistimi ea PCR ea Nako ea 'Nete ea BioRad CFX96 BioRad CFX Opus 96 Real-Nako PCR System