Sete ea ho Fumana Leukocyte Antigen B27 Nucleic Acid ea Motho

Tlhaloso e Khutšoanyane:

Sete ena e sebelisetsoa ho lemoha DNA ka boleng ba mefuta e fapaneng ea antigen ea leukocyte ea motho HLA-B*2702, HLA-B*2704 le HLA-B*2705.

Romella imeile ho rona

Qaqiso ea Sehlahisoa

Li-tag tsa Sehlahisoa

Lebitso la sehlahisoa

Kiti ea ho Fumana Sesosa sa Leukocyte ea Motho ea Antigen B27 ea Nucleic Acid (Fluorescence PCR) ea HWTS-GE011

Thuto ea mafu a seoa

Ankylosing spondylitis (AS) ke lefu le sa foleng le tsoelang pele la ho ruruha le hlaselang haholo-holo mokokotlo 'me le ka' na la ama manonyeletso a sacroiliac le manonyeletso a potolohileng ka tekanyo e fapaneng. Ho senotsoe hore AS e bontša ho kopana ho totobetseng ha lelapa 'me e amana haufi-ufi le antigen ea leukocyte ea motho HLA-B27. Ho batho, mefuta e fetang 70 ea mefuta ea HLA-B27 e fumanoe le ho tsejoa, 'me ho eona, HLA-B*2702, HLA-B*2704 le HLA-B*2705 ke mefuta e tloaelehileng haholo e amanang le lefu lena. Chaena, Singapore, Japane le setereke sa Taiwan sa Chaena, mofuta o tloaelehileng haholo oa HLA-B27 ke HLA-B*2704, e etsang hoo e ka bang 54%, e lateloa ke HLA-B*2705, e etsang hoo e ka bang 41%. Sete ena e ka lemoha DNA ka mefuta e fapaneng ea HLA-B*2702, HLA-B*2704 le HLA-B*2705, empa ha e li khetholle.

Mokero

FAM	HLA-B27
ROX	Taolo ea Ka Hare

Li-parameter tsa Tekheniki

Polokelo	Metsi: ≤-18℃
Nako ea ho phela	Metsi: Likhoeli tse 18
Mofuta oa Sampole	disampole tsa mali kaofela
Ct	≤40
CV	≤5.0%
LoD	1ng/μL
Ho hlaka	Liphetho tsa liteko tse fumanoeng ke kit ena li ke ke tsa angoa ke hemoglobin (<800g/L), bilirubin (<700μmol/L), le lipids tsa mali/triglycerides (<7mmol/L) maling.
Lisebelisoa tse Sebetsang	Mekhoa ea Li-biosystem tse Sebelisitsoeng 7500 Mekhoa ea PCR ea Nako ea Sebele Mekhoa ea Biosystem e Sebelisitsoeng ea StepOne Mekhoa ea PCR ea Nako ea 'Nete Leseli la ho Tsamaisa Li-Cycler^®Sistimi ea PCR ea Nako ea 'Nete ea 480 Sistimi ea Agilent-Stratagene Mx3000P Q-PCR