Phetoho ea Liphatsa tsa Lefutso ea EML4-ALK ea Motho
Lebitso la sehlahisoa
Kiti ea ho Lemoha Phetoho ea Liphatsa tsa Lefutso ea HWTS-TM006-Human EML4-ALK Fusion Gene (Fluorescence PCR)
Setifikeiti
TFDA
Thuto ea mafu a seoa
Sete ena e sebelisetsoa ho lemoha ka boleng mefuta e 12 ea liphatsa tsa lefutso tsa EML4-ALK fusion lisampoleng tsa bakuli ba kankere ea matšoafo ea lisele tse seng nyane tsa batho ka har'a vitro. Liphetho tsa liteko ke tsa litšupiso tsa bongaka feela 'me ha lia lokela ho sebelisoa e le motheo feela oa kalafo e ikhethileng ea bakuli. Lingaka li lokela ho etsa likahlolo tse felletseng liphethong tsa liteko ho latela lintlha tse kang boemo ba mokuli, matšoao a meriana, karabelo ea kalafo, le matšoao a mang a liteko tsa laboratori. Kankere ea matšoafo ke hlahala e kotsi e atileng haholo lefatšeng ka bophara, 'me 80% ~ 85% ea linyeoe ke kankere ea matšoafo ea lisele tse seng nyane (NSCLC). Ho kopanngoa ha liphatsa tsa lefutso tsa echinoderm microtubule-associated protein-like 4 (EML4) le anaplastic lymphoma kinase (ALK) ke sepheo se secha ho NSCLC, EML4 le ALK li fumaneha ka ho latellana ho batho lihlopha tsa P21 le P23 ho chromosome 2 'me li arotsoe ke lipara tse ka bang limilione tse 12.7 tsa motheo. Ho fumanoe bonyane mefuta e 20 ea ho kopanya, eo har'a eona ho tloaelehileng mefuta e 12 ea ho kopanya e fetotsoeng ho Tafole ea 1, moo mofuta oa 1 (E13; A20) e leng oona o atileng haholo, o lateloa ke mefuta e 3a le 3b (E6; A20), e ikarabellang bakeng sa hoo e ka bang 33% le 29% ea bakuli ba nang le EML4-ALK fusion gene NSCLC, ka ho latellana. Lithibela tsa ALK tse emeloang ke Crizotinib ke meriana e reretsoeng limolek'hule tse nyane e etselitsoeng liphetoho tsa ALK gene fusion. Ka ho thibela ts'ebetso ea sebaka sa ALK tyrosine kinase, ho thibela litsela tsa eona tse sa tloaelehang tsa ho supa, ka hona ho thibela kholo ea lisele tsa hlahala, ho fihlela phekolo e reretsoeng lihlahala. Lithuto tsa bongaka li bontšitse hore Crizotinib e na le sekhahla se sebetsang sa ho feta 61% ho bakuli ba nang le liphetoho tsa fusion tsa EML4-ALK, leha e se na phello ho bakuli ba mofuta oa naha. Ka hona, ho fumanoa ha phetoho ea fusion ea EML4-ALK ke motheo le motheo oa ho tataisa ts'ebeliso ea meriana ea Crizotinib.
Mokero
| FAM | Sesebediswa sa karabelo 1, 2 |
| VIC(HEX) | Sesebediswa sa karabelo 2 |
Li-parameter tsa Tekheniki
| Polokelo | ≤-18℃ |
| Nako ea ho phela | Likhoeli tse 9 |
| Mofuta oa Sampole | disampole tsa dinama tse tshwerweng ke parafini kapa dikarolo tse tshwerweng ke lefu |
| CV | ＜5.0% |
| Ct | ≤38 |
| LoD | Sete ena e ka lemoha liphetoho tsa motsoako tse fihlang ho likopi tse 20. |
| Lisebelisoa tse Sebetsang: | Mekhoa ea Li-biosystem tse Sebelisitsoeng 7500 Mekhoa ea PCR ea Nako ea Sebele Mekhoa ea PCR e Potlakileng ea Li-Biosystem tse 7500 tsa Nako ea 'Nete Mekhoa ea PCR ea SLAN ®-96P ea Nako ea 'Nete Mekhoa e 5 ea PCR ea QuantStudio™ ea Nako ea 'Nete Sistimi ea PCR ea LightCycler®480 ea Nako ea 'Nete Sistimi ea ho lemoha PCR ea LineGene 9600 Plus ea Nako ea 'Nete MA-6000 Nako ea Sebele ea Bongata ba Thermal Cycler Sistimi ea PCR ea BioRad CFX96 ea Nako ea 'Nete Sistimi ea PCR ea BioRad CFX Opus 96 ea Nako ea 'Nete |
Phallo ea Mosebetsi
Reagent e kgothaletswang ya ho ntsha: RNeasy FFPE Kit (73504) ka QIAGEN, Dikarolo tsa Dithishu tse kentsweng ka Paraffin Total RNA Extraction Kit (DP439) ka Tiangen Biotech(Beijing) Co.,Ltd.
