Motho EML4-ALK Fusion Gene Phetoho
Lebitso la sehlahisoa
HWTS-TM006-Human EML4-ALK Fusion Gene Mutation Detection Kit(Fluorescence PCR)
Setifikeiti
TFDA
Epidemiology
Setsi sena se sebelisetsoa ho lemoha ka mokhoa oa boleng mefuta e 12 ea phetoho ea EML4-ALK ea lefutso la fusion lisampong tsa bakuli ba mofetše oa matšoafo oa lisele tse seng nyane ka har'a vitro.Liphetho tsa liteko ke tsa litšupiso tsa bongaka feela 'me ha lia lokela ho sebelisoa e le eona feela motheo oa kalafo e ikhethileng ea bakuli.Baoki ba lokela ho etsa likahlolo tse felletseng mabapi le liphetho tsa liteko tse ipapisitseng le lintlha tse kang boemo ba mokuli, matšoao a lithethefatsi, karabelo ea kalafo, le matšoao a mang a tlhahlobo ea laboratori.Kankere ea matšoafo ke hlahala e kotsi ka ho fetisisa lefatšeng ka bophara, 'me 80% ~ 85% ea linyeoe ke mofets'e o seng o monyane oa lisele tsa matšoafo (NSCLC).Gene fusion ea echinoderm microtubule-associated protein-like 4 (EML4) le anaplastic lymphoma kinase (ALK) ke sepheo sa novel ho NSCLC, EML4 le ALK ka ho latellana li fumaneha ho batho lihlopha tsa P21 le P23 ho chromosome 2 'me li arotsoe ka hoo e ka bang 12.7 li-base pairs tse limilione.Bonyane ho fumanwe mefuta e 20 ya fusion, eo hara yona diphetoho tse 12 tsa fusion tse ho Lethathamo la 1 di atileng, moo phetoho 1 (E13; A20) e leng yona e tlwaelehileng, e latelwang ke mutants 3a le 3b (E6; A20), e nkang hoo e ka bang. 33% le 29% ea bakuli ba nang le EML4-ALK fusion gene NSCLC, ka ho latellana.ALK inhibitors e emeloang ke Crizotinib ke lithethefatsi tse lebisitsoeng ho molek'hule tse nyane tse etselitsoeng liphetoho tsa ALK gene fusion.Ka ho thibela ts'ebetso ea sebaka sa ALK tyrosine kinase, ho thibela litsela tsa eona tse sa tloaelehang tsa matšoao, ka hona ho thibela ho hōla ha lisele tsa tumor, ho finyella phekolo e lebisitsoeng bakeng sa lihlahala.Liphuputso tsa bongaka li bontšitse hore Crizotinib e na le tekanyo e sebetsang ea ho feta 61% ho bakuli ba nang le liphetoho tsa fusion ea EML4-ALK, ha e batla e se na phello ho bakuli ba mofuta o hlaha.Ka hona, ho lemoha phetoho ea EML4-ALK fusion ke motheo le motheo oa ho tataisa tšebeliso ea lithethefatsi tsa Crizotinib.
Channel
| FAM | Sebaka sa karabelo 1, 2 |
| VIC(HEX) | Rection buffer 2 |
Tekheniki Parameters
| Polokelo | ≤-18℃ |
| Shelf-bophelo | Likhoeli tse 9 |
| Mofuta oa Mohlala | parafine e kentsoeng ka lisele tsa pathological kapa disampole tsa karolo |
| CV | 5.0% |
| Ct | ≤38 |
| LoD | Sesebelisoa sena se khona ho bona liphetoho tsa fusion tse ka tlase ho likopi tse 20. |
| Lisebelisoa tse Sebetsang: | Applied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7500 Fast Real-Time PCR Systems SLAN ®-96P ea Nako ea 'Nete PCR Systems QuantStudio™ 5 ea Nako ea 'Nete ea PCR Systems Sesebelisoa sa PCR sa LightCycler®480 Real-Time LineGene 9600 Plus Nako ea 'Nete ea PCR ea ho Fumana Sistimi MA-6000 Real-Nako Quantitative Thermal Cycler Sistimi ea PCR ea Nako ea 'Nete ea BioRad CFX96 BioRad CFX Opus 96 Real-Nako PCR System |
Phallo ea Mosebetsi
Sehlahisoa se khothalelitsoeng sa ho ntšoa: RNeasy FFPE Kit (73504) ka QIAGEN, Parafini-embedded Tissue Sections Total RNA Extraction Kit(DP439) by Tiangen Biotech(Beijing) Co.,Ltd.
