Motho EGFR Gene 29 Mutations
Lebitso la sehlahisoa
HWTS-TM001A-EGFR ea Motho Gene 29 Mutations Detection Kit (Fluorescence PCR)
Epidemiology
Kankere ea matšoafo e fetohile sesosa se ka sehloohong sa lefu la mofetše lefatšeng ka bophara, e sokelang bophelo bo botle ba batho haholo.Kankere e seng e nyane ea lisele ea matšoafo e etsa karolo ea 80 lekholong ea bakuli ba mofetše oa matšoafo.Hona joale EGFR ke sepheo sa bohlokoa ka ho fetisisa sa limolek'hule bakeng sa phekolo ea kankere e seng e nyenyane ea lisele tsa matšoafo.Phosphorylation ea EGFR e ka khothaletsa kholo ea lisele tsa tumor, phapang, tlhaselo, metastasis, anti-apoptosis, le ho khothaletsa angiogenesis ea tumor.EGFR tyrosine kinase inhibitors (TKI) e ka thibela tsela ea EGFR ea pontšo ka ho thibela EGFR autophosphorylation, kahoo e thibela ho ata le ho arohana ha lisele tsa tumor, ho khothalletsa tumor cell apoptosis, ho fokotsa tumor angiogenesis, joalo-joalo, e le ho finyella phekolo e lebisitsoeng ho hlahala.Liphuputso tse ngata li bontšitse hore katleho ea phekolo ea EGFR-TKI e amana haufi-ufi le boemo ba phetoho ea liphatsa tsa lefutso tsa EGFR, 'me e ka thibela ka ho khetheha ho hōla ha lisele tsa tumor ka phetoho ea liphatsa tsa lefutso tsa EGFR.Mofuta oa EGFR o letsohong le lekhutšoanyane la chromosome 7 (7p12), e bolelele bo felletseng ba 200Kb mme e na le li-exons tse 28.Sebaka se fetotsoeng se fumaneha haholo ho li-exons 18 ho isa ho 21, li-codons 746 ho isa ho tse 753 tse hlakotsoeng ho li-account tsa exon 19 tse ka bang 45% mme phetoho ea L858R ho exon 21 e nka 40% ho isa ho 45%.Litaelo tsa NCCN bakeng sa Tlhahlobo le Kalafo ea Kankere e Seng e Nyenyane ea Matšoafo ea Cell e bolela ka ho hlaka hore tlhahlobo ea phetoho ea liphatsa tsa lefutso ea EGFR e hlokahala pele ho tsamaiso ea EGFR-TKI.Setsi sena sa liteko se sebelisetsoa ho tataisa tsamaiso ea litlhare tsa epidermal growth factor receptor tyrosine kinase inhibitor (EGFR-TKI), le ho fana ka motheo oa meriana e ikemetseng bakeng sa bakuli ba nang le mofetše oa matšoafo oa sele e seng nyane.Setsi sena se sebelisoa feela bakeng sa ho lemoha liphetoho tse tloaelehileng mofuteng oa EGFR ho bakuli ba nang le kankere e seng e nyenyane ea kankere ea matšoafo.Liphetho tsa liteko ke tsa litšupiso tsa bongaka feela 'me ha lia lokela ho sebelisoa e le eona feela motheo oa kalafo e ikhethileng ea bakuli.Baoki ba lokela ho nahana ka boemo ba mokuli, matšoao a lithethefatsi, le kalafo Karabelo le matšoao a mang a tlhahlobo ea laboratori le lintlha tse ling li sebelisoa ho ahlola liphetho tsa liteko ka botlalo.
Channel
| FAM | IC Reaction Buffer, L858R Reaction Buffer, 19del Reaction Buffer, T790M Reaction Buffer, G719X Reaction Buffer, 3Ins20 Reaction Buffer, L861Q Reaction Buffer, S768I Reaction Buffer |
Tekheniki Parameters
| Polokelo | Mokelikeli: ≤-18℃ Lefifing;Lyophilized: ≤30℃ Lefifing |
| Shelf-bophelo | Mokelikeli: likhoeli tse 9;Lyophilized: likhoeli tse 12 |
| Mofuta oa Mohlala | lisele tse ncha tsa hlahala, karolo e hoammeng ea mafu, lisele tse kentsoeng ke parafine kapa karolo, plasma kapa serum. |
| CV | 5.0% |
| LoD | ho fumana tharollo ea nucleic acid ka tlas'a semelo sa 3ng/μL ea mofuta o hlaha, e khona ho bona sekhahla sa phetoho ea 1%. |
| Tse khethehileng | Ha ho na cross-reactivity le mofuta oa hlaha oa genomic DNA ea motho le mefuta e meng e fetotsoeng |
| Lisebelisoa tse sebetsang | Applied Biosystems 7500 Real-Time PCR SystemsApplied Biosystems 7300 Real-Time PCR Systems QuantStudio® 5 ea Nako ea 'Nete ea PCR Systems Sistimi ea PCR ea LightCycler® 480 ea Nako ea 'Nete Sistimi ea PCR ea Nako ea 'Nete ea BioRad CFX96 |
Phallo ea Mosebetsi
