Liphetoho tsa lefutso tsa EGFR Gene 29 tsa motho
Lebitso la sehlahisoa
HWTS-TM0012A-Human EGFR Gene 29 Transtations Detection Kit (Fluorescence PCR)
Thuto ea mafu a seoa
Kankere ea matšoafo e fetohile sesosa se seholo sa mafu a kankere lefatšeng ka bophara, e leng se behang bophelo bo botle ba batho kotsing e kholo. Kankere ea matšoafo e seng ea lisele tse nyane e etsa hoo e ka bang 80% ea bakuli ba kankere ea matšoafo. EGFR hajoale ke sepheo sa bohlokoa ka ho fetisisa sa limolek'hule bakeng sa kalafo ea kankere ea matšoafo e seng ea lisele tse nyane. Phosphorylation ea EGFR e ka khothaletsa kholo ea lisele tsa hlahala, phapang, tlhaselo, metastasis, anti-apoptosis, le ho khothaletsa angiogenesis ea hlahala. EGFR tyrosine kinase inhibitors (TKI) e ka thibela tsela ea lipontšo tsa EGFR ka ho thibela autophosphorylation ea EGFR, ka hona e thibela ho ata le ho fapana ha lisele tsa hlahala, e khothalletsa apoptosis ea lisele tsa hlahala, e fokotsa angiogenesis ea hlahala, jj., e le ho fihlela phekolo e shebaneng le hlahala. Palo e kholo ea lithuto e bontšitse hore katleho ea kalafo ea EGFR-TKI e amana haufi-ufi le boemo ba phetoho ea liphatsa tsa lefutso tsa EGFR, 'me e ka thibela ka ho khetheha kholo ea lisele tsa hlahala ka phetoho ea liphatsa tsa lefutso tsa EGFR. Jini ea EGFR e fumaneha letsohong le lekhutšoanyane la chromosome 7 (7p12), e bolelele bo felletseng ba 200Kb 'me e na le li-exons tse 28. Sebaka se fetotsoeng se fumaneha haholo-holo ho li-exons tse 18 ho isa ho tse 21, li-codon tse 746 ho isa ho tse 753 tse tlosoang ho exon 19 li etsa hoo e ka bang 45% 'me phetoho ea L858R ho exon 21 e etsa hoo e ka bang 40% ho isa ho 45%. Tataiso ea NCCN bakeng sa Tlhahlobo le Kalafo ea Kankere ea Matšoafo ea Sele e Seng Nyenyane e bolela ka ho hlaka hore tlhahlobo ea phetoho ea liphatsa tsa lefutso ea EGFR ea hlokahala pele ho tsamaiso ea EGFR-TKI. Kiti ena ea teko e sebelisoa ho tataisa tsamaiso ea meriana ea epidermal growth factor receptor tyrosine kinase inhibitor (EGFR-TKI), 'me e fana ka motheo oa meriana e ikhethileng bakeng sa bakuli ba nang le kankere ea matšoafo ea sele e seng nyane. Kiti ena e sebelisoa feela bakeng sa ho lemoha liphetoho tse tloaelehileng ho jini ea EGFR ho bakuli ba nang le kankere ea matšoafo ea sele e seng nyane. Liphetho tsa liteko ke tsa litšupiso tsa bongaka feela 'me ha lia lokela ho sebelisoa e le motheo feela oa kalafo e ikhethileng ea bakuli. Lingaka li lokela ho nahana ka boemo ba mokuli, matšoao a meriana le kalafo. Karabelo le matšoao a mang a liteko tsa laboratori le lintlha tse ling li sebelisoa ho ahlola ka botlalo liphetho tsa liteko.
Mokero
| FAM | IC Reaction Buffer, L858R Reaction Buffer, 19del Reaction Buffer, T790M Reaction Buffer, G719X Reaction Buffer, 3Ins20 Reaction Buffer, L861Q Reaction Buffer, S768I Reaction Buffer |
Li-parameter tsa Tekheniki
| Polokelo | Metsi: ≤-18℃ Lefifing; Le pholile: ≤30℃ Lefifing |
| Nako ea ho phela | Metsi: likhoeli tse 9; Lyophilized: likhoeli tse 12 |
| Mofuta oa Sampole | lisele tse foreshe tsa hlahala, karolo e hoammeng ea lefu, lisele kapa karolo ea lefu e kentsoeng ka parafini, plasma kapa serum |
| CV | ＜5.0% |
| LoD | ho lemoha tharollo ea karabelo ea nucleic acid tlas'a semelo sa mofuta oa tlhaho oa 3ng/μL, ho ka lemoha sekhahla sa phetoho ea 1% ka mokhoa o tsitsitseng |
| Ho hlaka | Ha ho na phetoho e kopanetsoeng le DNA ea genomic ea motho ea mofuta oa tlhaho le mefuta e meng ea liphetoho tse fetotsoeng. |
| Lisebelisoa tse Sebetsang | Mekhoa ea Li-biosystem tse Sebelisitsoeng 7500 Mekhoa ea PCR ea Nako ea SebeleMekhoa ea Li-biosystem tse Sebelisitsoeng 7300 Mekhoa ea PCR ea Nako ea Sebele Mekhoa ea PCR ea QuantStudio® 5 ea Nako ea 'Nete Sistimi ea PCR ea LightCycler® 480 ea Nako ea 'Nete Sistimi ea PCR ea BioRad CFX96 ea Nako ea 'Nete |
Phallo ea Mosebetsi
