Phetoho ea Gene ea BRAF ea Motho V600E
Lebitso la sehlahisoa
HWTS-TM007-Human BRAF Gene V600E Transformation Set (Fluorescence PCR)
Setifikeiti
CE/TFDA
Thuto ea mafu a seoa
Ho fumanoe mefuta e fetang 30 ea liphetoho tsa BRAF, tseo hoo e ka bang 90% ea tsona li fumanehang ho exon 15, moo phetoho ea V600E e nkoang e le phetoho e tloaelehileng haholo, ke hore, thymine(T) boemong ba 1799 ho exon 15 e fetoloang ho ba adenine (A), e leng se fellang ka hore valine (V) e nkeloe sebaka ke glutamic acid (E) sebakeng sa 600 sehlahisoa sa protheine. Liphetoho tsa BRAF hangata li fumanoa lihlahleng tse kotsi tse kang melanoma, kankere ea mala a maholo, kankere ea thyroid le kankere ea matšoafo. Ho utloisisa phetoho ea liphatsa tsa lefutso tsa BRAF e se e le tlhoko ea ho hlahloba meriana e shebaneng le phetoho ea liphatsa tsa lefutso tsa EGFR-TKIs le BRAF kalafong ea meriana e shebaneng le bongaka bakeng sa bakuli ba ka ruang molemo.
Mokero
| FAM | Phetoho ea V600E, taolo ea ka hare |
Li-parameter tsa Tekheniki
| Polokelo | ≤-18℃ |
| Nako ea ho phela | Likhoeli tse 9 |
| Mofuta oa Sampole | disampole tsa dinama tse nang le bothata tse kentsweng ka parafini |
| CV | ＜5.0% |
| Ct | ≤38 |
| LoD | Sebelisa lisebelisoa ho lemoha taolo ea boleng ba LoD e tsamaellanang. a) Tlas'a semelo sa mofuta oa tlhaho sa 3ng/μL, sekhahla sa phetoho ea 1% se ka fumanoa ka har'a buffer ea karabelo ka mokhoa o tsitsitseng; b) sekhahla sa phetoho ea 1%, phetoho ea 1×103Likopi/mL ka morao ho mofuta oa tlhaho oa 1×105Likopi/mL li ka fumanoa ka mokhoa o tsitsitseng ka har'a sesebelisoa sa ho thibela karabelo; c) IC Reaction Buffer e ka lemoha taolo ea boleng ba SW3 e tlase ka ho fetisisa ea taolo ea ka hare ea k'hamphani. |
| Lisebelisoa tse Sebetsang: | Mekhoa ea Li-biosystem tse Sebelisitsoeng 7500 Mekhoa ea PCR ea Nako ea SebeleLisebelisoa tsa Biosystem tse Sebelisitsoeng 7300 PCR ea Nako ea Sebele Mekhoa, QuantStudio® 5 Mekhoa ea PCR ea Nako ea 'Nete Sistimi ea PCR ea LightCycler®480 ea Nako ea 'Nete Sistimi ea PCR ea BioRad CFX96 ea Nako ea 'Nete |
Phallo ea Mosebetsi
Di-reagent tse kgothalletswang ho ntsha: QIAGEN's QIAamp DNA FFPE Tissue Kit (56404), Paraffin-embedded Tissue DNA Rapid Extraction Kit (DP330) e hlahisitsweng ke Tiangen Biotech(Beijing) Co., Ltd.
