Motho BCR-ABL Fusion Gene Phetoho
Lebitso la sehlahisoa
HWTS-GE010A-Human BCR-ABL Fusion Gene Mutation Detection Kit (Fluorescence PCR)
Epidemiology
Chronic myelogenousleukemia (CML) ke lefu le kotsi la clonal la lisele tsa stem tsa hematopoietic.Ho feta 95% ea bakuli ba CML ba na le chromosome ea Philadelphia (Ph) liseleng tsa bona tsa mali.Pathogenesis e kholo ea CML ke e latelang: Mofuta oa fusion oa BCR-ABL o thehoa ke phetoho pakeng tsa abl proto-oncogene (Abelson murine leukemia viral oncogene homolog 1) letsohong le lelelele la chromosome 9 (9q34) le sebaka sa lihlopha tsa breakpoint ( BCR) gene letsohong le lelelele la chromosome 22 (22q11);protheine ea fusion e kenyellelitsoeng ke mofuta ona e na le ts'ebetso ea tyrosine kinase (TK), 'me e kenya tšebetsong litsela tsa eona tse tlase (tse kang RAS, PI3K, le JAK/STAT) ho khothaletsa karohano ea lisele le ho thibela apoptosis ea lisele, ho etsa hore lisele li ate hampe, ka hona li baka ketsahalo ea CML.BCR-ABL ke e 'ngoe ea matšoao a bohlokoa a tlhahlobo ea CML.Phetoho e matla ea boemo ba eona ba sengoloa ke sesupo se ka tšeptjoang bakeng sa kahlolo ea pele ea leukemia mme e ka sebelisoa ho bolela esale pele ho khutla ha leukemia kamora kalafo.
Channel
| FAM | Mofuta oa motsoako oa BCR-ABL |
| VIC/HEX | Taolo ea ka hare |
Tekheniki Parameters
| Polokelo | Mokelikeli: ≤-18℃ Lefifing |
| Shelf-bophelo | Mokelikeli: Likhoeli tse 9 |
| Mofuta oa Mohlala | Mehlala ea moko oa masapo |
| LoD | 1000 Likopi / mL |
| Tse khethehileng
| Ha ho na cross-reactivity le mefuta e meng ea fusion TEL-AML1, E2A-PBX1, MLL-AF4, AML1-ETO, le PML-RARa. |
| Lisebelisoa tse sebetsang | Applied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio® 5 ea Nako ea 'Nete ea PCR Systems Litsamaiso tsa PCR tsa nako ea sebele tsa SLAN-96P Sesebelisoa sa PCR sa LightCycler®480 Real-Time LineGene 9600 Plus Nako ea 'Nete ea PCR ea ho Fumana Sistimi MA-6000 Real-Nako Quantitative Thermal Cycler Sistimi ea PCR ea Nako ea 'Nete ea BioRad CFX96 BioRad CFX Opus 96 Real-Nako PCR System |
