Phetoho ea liphatsa tsa lefutso tsa BCR-ABL Fusion ea Motho
Lebitso la sehlahisoa
Kiti ea ho Lemoha Phetoho ea Liphatsa tsa Lefutso ea HWTS-GE010A-Human BCR-ABL Fusion Gene Mutation (Fluorescence PCR)
HWTS-GE016A-Sesebelisoa sa ho Fumana Phetoho ea Liphatsa tsa lefutso sa BCR-ABL se omisitsoeng ka ho Hatsetsa (Fluorescence PCR)
Thuto ea mafu a seoa
Lefu la myelogenousleukemia le sa foleng (CML) ke lefu le kotsi la clonal la lisele tsa hematopoietic stem. Bakuli ba fetang 95% ba CML ba na le chromosome ea Philadelphia (Ph) liseleng tsa bona tsa mali. Pathogene e ka sehloohong ea CML ke e latelang: Jini ea fusion ea BCR-ABL e thehoa ke translocation pakeng tsa abl proto-oncogene (Abelson murine leukemia viral oncogene homolog 1) letsohong le lelelele la chromosome 9 (9q34) le gene ea breakpoint cluster region (BCR) letsohong le lelelele la chromosome 22 (22q11); protheine ea fusion e kentsoeng ke gene ena e na le ts'ebetso ea tyrosine kinase (TK), 'me e kenya tšebetsong litsela tsa eona tsa lipontšo tse theohelang (joalo ka RAS, PI3K, le JAK/STAT) ho khothaletsa karohano ea sele le ho thibela apoptosis ea sele, e etsang hore lisele li ate ka mokhoa o kotsi, 'me ka hona li bake ho hlaha ha CML. BCR-ABL ke e 'ngoe ea matšoao a bohlokoa a tlhahlobo ea CML. Phetoho e matla ea boemo ba eona ba tlaleho ke sesupo se tšepahalang sa kahlolo ea pele ea leukemia 'me e ka sebelisoa ho bolela esale pele ho khutla ha leukemia kamora kalafo.
Mokero
| FAM | Jini ea motsoako oa BCR-ABL |
| VIC/HEX | Taolo ea Ka Hare |
Li-parameter tsa Tekheniki
| Polokelo | Metsi: ≤-18℃ Lefifing |
| Nako ea ho phela | Metsi: likhoeli tse 9 |
| Mofuta oa Sampole | Disampole tsa moko oa masapo |
| LoD | Likopi tse 1000/mL |
| Ho hlaka
| Ha ho na karabelo e ts'oanang le liphatsa tse ling tsa lefutso la fusion TEL-AML1, E2A-PBX1, MLL-AF4, AML1-ETO, le PML-RARa. |
| Lisebelisoa tse Sebetsang | Mekhoa ea Li-biosystem tse Sebelisitsoeng 7500 Mekhoa ea PCR ea Nako ea Sebele Mekhoa ea PCR e Potlakileng ea Li-Biosystem tse 7500 tsa Nako ea 'Nete Mekhoa ea PCR ea QuantStudio® 5 ea Nako ea 'Nete Mekhoa ea PCR ea Nako ea Sebele ea SLAN-96P Sistimi ea PCR ea LightCycler®480 ea Nako ea 'Nete Sistimi ea ho lemoha PCR ea LineGene 9600 Plus ea Nako ea 'Nete MA-6000 Nako ea Sebele ea Bongata ba Thermal Cycler Sistimi ea PCR ea BioRad CFX96 ea Nako ea 'Nete Sistimi ea PCR ea BioRad CFX Opus 96 ea Nako ea 'Nete |
