Vaerase ea Hepatitis B DNA ea Bongata ba Fluorescence
Lebitso la sehlahisoa
Kit ea Tlhahlobo ea Fluorescence ea HWTS-HP015 Hepatitis B Virus DNA Quantitative Fluorescence (Fluorescence PCR)
Thuto ea mafu a seoa
Hepatitis B ke lefu le bakoang ke kokoana-hloko ea hepatitis B (HBV), e khetholloang haholo-holo ke liso tsa ho ruruha ha sebete, 'me e ka baka tšenyo e ngata lithong. Bakuli ba Hepatitis B ba iponahatsa ka bongaka e le mokhathala, ho lahleheloa ke takatso ea lijo, ho ruruha ha litho tse ka tlase kapa ka kakaretso, le hepatomegaly ka lebaka la ho se sebetse hantle ha sebete. Karolo ea bohlano lekholong ea batho ba baholo ba tšoaelitsoeng le 95% ea batho ba tšoaelitsoeng ba otlolohile ba sitoa ho phekola HBV ka katleho, e leng se fellang ka tšoaetso e sa feleng ea kokoana-hloko, 'me tšoaetso e meng e sa foleng qetellong e fetoha cirrhosis ea sebete le carcinoma ea hepatocellular.[1-4].
Mokero
| FAM | HBV-DNA |
| ROX | Taolo ea Ka Hare |
Li-parameter tsa Tekheniki
| Polokelo | ≤-18℃ |
| Nako ea ho phela | Likhoeli tse 12 |
| Mofuta oa Sampole | serum e foreshe, Plasma |
| Tt | ≤42 |
| CV | ≤5.0% |
| LoD | 5 IU/mL |
| Ho hlaka | Liphetho tsa ho ikhetha li bontša hore linyeoe tsohle tse 50 tsa lisampole tsa serum tse phetseng hantle tsa HBV DNA tse se nang tšoaetso ha li na tšoaetso; liphetho tsa liteko tsa ho kopanya li bontša hore ha ho na karabelo e kopaneng pakeng tsa kit ena le livaerase tse ling (HAV, HCV, DFV, HIV) bakeng sa ho lemoha nucleic acid ka lisampole tsa mali, le li-genome tsa batho. |
| Lisebelisoa tse Sebetsang | Sistimi ea PCR ea Nako ea 'Nete e Sebelisitsoeng ea 7500 Mekhoa ea PCR e Potlakileng ea Li-Biosystem tse 7500 tsa Nako ea 'Nete Mekhoa ea PCR ea QuantStudio®5 ea Nako ea 'Nete Mekhoa ea PCR ea Nako ea Sebele ea SLAN-96P (Hongshi Medical Technology Co., Ltd.) Sistimi ea PCR ea LightCycler®480 ea Nako ea 'Nete Sistimi ea ho lemoha PCR ea LineGene 9600 Plus ea Nako ea 'Nete (FQD-96A, theknoloji ea Hangzhou Bioer) Sesebelisi sa Thermal sa MA-6000 sa Nako ea Sebele sa Bongata ba Thermal (Suzhou Molarray Co., Ltd.) Sistimi ea PCR ea BioRad CFX96 ea Nako ea 'Nete Sistimi ea PCR ea BioRad CFX Opus 96 ea Nako ea 'Nete |
Phallo ea Mosebetsi
Reagent e kgothalletswang ho ntsha: Macro & Micro-Test Virus DNA/RNA Kit (HWTS-3017) (e ka sebediswang le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) ke Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.. Ho ntsha ho lokela ho etswa ho ya ka bukana ya ditaelo, bophahamo ba sampole e ntshitsweng ke 300μL, mme bophahamo ba elution bo kgothaletswang ke 70μL.
