Hepatitis B Virus DNA Quantitative Fluorescence
Lebitso la sehlahisoa
HWTS-HP015 Hepatitis B Virus DNA Quantitative Fluorescence Diagnostic Kit (Fluorescence PCR)
Epidemiology
Hepatitis B ke lefu le bakoang ke kokoana-hloko ea hepatitis B (HBV), e khetholloang haholo ke maqeba a ho ruruha sebeteng, 'me e ka baka tšenyo ea litho tse ngata.Bakuli ba Hepatitis B ba bonahala ka tleliniking e le mokhathala, ho lahleheloa ke takatso ea lijo, karolo e tlaase kapa edema e akaretsang, le hepatomegaly ka lebaka la ho se sebetse ha sebete.Liperesente tse hlano tsa batho ba baholo ba tšoaelitsoeng le 95% ea batho ba tšoaelitsoeng holimo ha ba khone ho hlakola HBV ka nepo, e leng se bakang tšoaetso ea kokoana-hloko e sa khaotseng, 'me mafu a mang a sa foleng a qetella a fetoha sebete sa sebete le hepatocellular carcinoma.[1-4].
Channel
FAM | HBV-DNA |
ROX | Taolo ea ka hare |
Tekheniki Parameters
Polokelo | ≤-18℃ |
Shelf-bophelo | Likhoeli tse 12 |
Mofuta oa Mohlala | serum e ncha, Plasma |
Tt | ≤42 |
CV | ≤5.0% |
LoD | 5 IU/mL |
Tse khethehileng | Liphetho tse ikhethang li bontša hore linyeoe tsohle tse 50 tsa lisampole tse mpe tsa serum ea HBV DNA li mpe;liphetho tsa liteko tsa cross-reactivity li bonts'a hore ha ho na tšebelisano lipakeng tsa khithi ena le livaerase tse ling (HAV, HCV, DFV, HIV) bakeng sa ho sibolla nucleic acid ka lisampole tsa mali, le liphatsa tsa lefutso tsa batho. |
Lisebelisoa tse sebetsang | Lisebelisoa tsa Biosystems 7500 tsa Nako ea 'Nete ea PCR Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Nako ea 'Nete ea PCR Systems SLAN-96P ea Nako ea Sebele PCR Systems(Hongshi Medical Technology Co., Ltd.) Sesebelisoa sa PCR sa LightCycler®480 Real-Time LineGene 9600 Plus Nako ea 'Nete ea PCR Detection System (FQD-96A, theknoloji ea Hangzhou Bioer) MA-6000 Nako ea 'Nete ea Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) Sistimi ea PCR ea Nako ea 'Nete ea BioRad CFX96 BioRad CFX Opus 96 Real-Nako PCR System |
Phallo ea Mosebetsi
Khetho ea 1.
Macro & Micro-Test General DNA/RNA Kit (HWTS-3017) le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).Ho ntšoa ho lokela ho etsoa ho latela bukana ea litaelo, molumo oa sampole o ntšitsoeng ke 300μL, 'me molumo o khothaletsoang oa elution ke 70μL.