Zaire e omisitsoeng ka leqhoa le Sudan Ebolavirus Nucleic Acid
Lebitso la sehlahisoa
HWTS-FE035-Freeze-Omisitsoeng Zaire le Sudan Ebolavirus Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Ebolavirus ke ea Filoviridae, e leng kokoana-hloko ea RNA ea khoele e le 'ngoe e sa arotsoeng. Livaerase ke likhoele tse telele tse bolelele ba virion bo bolelele ba 1000nm le bophara ba 100nm. Genome ea Ebolavirus ke RNA e sa arotsoeng e nang le mefuta e mebe e nang le boholo ba 18.9kb, e kenyelletsang liprotheine tse 7 tsa meralo le protheine e le 'ngoe e se nang sebopeho. Ebolavirus e ka aroloa ka mefuta e kang Zaire, Sudan, Bundibugyo, Tai Forest le Reston. Har'a bona, mofuta oa Zaire le oa Sudan ho tlalehiloe hore li bolaile batho ba bangata ka lebaka la tšoaetso. EHF (Ebola Hemorrhagic Fever) ke lefu le tšoaetsanoang le bakoang ke kokoana-hloko ea Ebola. Batho ba tšoaelitsoe haholo-holo ka ho kopana le maro a 'mele, ho tsoa le mantle a bakuli kapa liphoofolo tse nang le tšoaetso,' me lipontšo tsa kliniki ke haholo-holo feberu e hlahelang, ho tsoa mali le tšenyo ea litho tse ngata. EHF e na le sekhahla se phahameng sa lefu la 50% -90%. Hona joale, mekhoa ea ho hlahloba Ebolavirus haholo-holo ke liteko tsa laboratori, ho kenyelletsa le likarolo tse peli: ho lemoha etiological le ho lemoha serological. Ho lemoha lintho tsa tlhaho ho kenyelletsa ho lemoha li-antigen tsa kokoana-hloko ka lisampole tsa mali ka ELISA, ho lemoha li-nucleic acid ka mekhoa ea ho hōlisa joaloka RT-PCR, joalo-joalo, le ho sebelisa lisele tsa Vero, Hela, joalo-joalo bakeng sa ho itšehla thajana le setso. Ho lemoha ha serological ho kenyelletsa ho lemoha li-antibodies tsa IgM tsa Serum ka ho tšoara ELISA, le ho lemoha li-antibodies tsa IgG tsa Serum ka ELISA, immunofluorescence, joalo-joalo.
Tekheniki Parameters
| Polokelo | ≤30 ℃ |
| Shelf-bophelo | Likhoeli tse 12 |
| Mofuta oa Mohlala | serum, plamehlala ea sma |
| CV | ≤5.0% |
| LoD | 500 Likopi / μL |
| Lisebelisoa tse sebetsang | E sebetsa ho mochini oa ho lemoha oa mofuta oa I: Applied Biosystems 7500 Real-Nako PCR Systems, QuantStudio®Litsamaiso tse 5 tsa nako ea 'nete tsa PCR, SLAN-96P ea Nako ea Sebele PCR Systems (Hongshi Medical Technology Co., Ltd.), LineGene 9600 Plus Nako ea 'Nete ea PCR Detection Systems (FQD-96A, theknoloji ea Hangzhou Bioer), MA-6000 Real-Nako Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.), BioRad CFX96 Real-Time PCR System, BioRad CFX Opus 96 Real-Time PCR System. E sebetsa ho mofuta oa II reagent ea ho lemoha: EudemonTMAIO800 (HWTS-EQ007) ka Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. |
Phallo ea Mosebetsi
Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3001, HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006). Ho ntšoa ho lokela ho etsoa ho latela litaelo, 'me molumo oa sampole o ntšitsoeng ke 200μL mme molumo o khothaletsoang oa elution ke 80μL.
