Zaire e omisitsoeng ka serame le Sudan Ebolavirus Nucleic Acid

Tlhaloso e Khutšoanyane:

Sete ena e loketse ho lemoha nucleic acid ea Ebolavirus lisampoleng tsa serum kapa plasma tsa bakuli ba belaelloang hore ba na le tšoaetso ea Zaire ebolavirus (EBOV-Z) le Sudan ebolavirus (EBOV-S), ho lemoha ho thaepa ha eona.


Qaqiso ea Sehlahisoa

Li-tag tsa Sehlahisoa

Lebitso la sehlahisoa

HWTS-FE035-Sesebelisoa sa ho Fumana Asiti ea Nucleic Acid sa Zaire le Sudan se omisitsoeng ka ho Hatsetsa (Fluorescence PCR)

Thuto ea mafu a seoa

Ebolavirus ke ea Filoviridae, e leng kokoana-hloko ea RNA e se nang likarolo tse le 'ngoe e se nang likarolo tse le 'ngoe. Livaerase ke likhoele tse telele tse bolelele ba virion bo tloaelehileng ba 1000nm le bophara ba hoo e ka bang 100nm. Jenome ea kokoana-hloko ea Ebola ke RNA e se nang likarolo tse le 'ngoe e se nang likarolo tse le 'ngoe e nang le boholo ba 18.9kb, e kenyang liprotheine tse 7 tsa sebopeho le protheine e le 'ngoe e se nang sebopeho. Ebolavirus e ka aroloa ka mefuta e kang Zaire, Sudan, Bundibugyo, Tai Forest le Reston. Har'a tsona, mofuta oa Zaire le mofuta oa Sudan li tlalehiloe li baka mafu a batho ba bangata ka lebaka la tšoaetso. EHF (Ebola Hemorrhagic Fever) ke lefu le tšoaetsanoang le bakoang ke kokoana-hloko ea Ebola. Batho ba tšoaetsoa haholo-holo ke ho kopana le maro a 'mele, mantle le mantle a bakuli kapa liphoofolo tse tšoaelitsoeng, 'me matšoao a kliniki ke feberu e hlahellang, ho tsoa mali le tšenyo e ngata ea litho. EHF e na le sekhahla se phahameng sa lefu sa 50%-90%. Hona jwale, mekgwa ya ho fumana Ebolavirus ke haholo-holo diteko tsa laboratori, ho kenyeletswa le dintlha tse pedi: ho lemoha etiological le ho lemoha serological. Ho lemoha etiological ho kenyeletsa ho lemoha di-antigen tsa vaerase disampoleng tsa madi ka ELISA, ho lemoha nucleic acid ka mekgwa ya ho eketsa matla jwalo ka RT-PCR, jj., le ho sebedisa disele tsa Vero, Hela, jj. bakeng sa ho arola le ho hodisa vaerase. Ho lemoha serological ho kenyeletsa ho lemoha di-antibodies tsa Serum specific IgM ka ho tshwara ELISA, le ho lemoha di-antibodies tsa Serum specific IgG ka ELISA, immunofluorescence, jj.

Li-parameter tsa Tekheniki

Polokelo

≤30℃

Nako ea ho phela Likhoeli tse 12
Mofuta oa Sampole serum, pladisampole tsa sma
CV ≤5.0%
LoD Likopi tse 500/μL
Lisebelisoa tse Sebetsang E sebetsa ho reagent ea ho lemoha mofuta oa I:

Mekhoa ea Li-biosystem tse Sebelisitsoeng 7500 Mekhoa ea PCR ea Nako ea 'Nete,

QuantStudio®Mekhoa e 5 ea PCR ea Nako ea 'Nete,

Mekhoa ea PCR ea Nako ea 'Nete ea SLAN-96P (Hongshi Medical Technology Co., Ltd.),

LineGene 9600 Plus Mekhoa ea ho Lemoha PCR ea Nako ea 'Nete (FQD-96A, theknoloji ea Hangzhou Bioer),

Sesebelisi sa Thermal sa MA-6000 sa Nako ea 'Nete sa Bongata ba Thermal (Suzhou Molarray Co., Ltd.),

Sistimi ea PCR ea BioRad CFX96 ea Nako ea 'Nete, Sistimi ea PCR ea BioRad CFX Opus 96 ea Nako ea 'Nete.

E sebetsa ho reagent ea ho lemoha mofuta oa II:

EudemonTMAIO800 (HWTS-EQ007) ke Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.

Phallo ea Mosebetsi

Sete ea Viral DNA/RNA ea Macro & Micro-Test Viral (HWTS-3001, HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006). Ho ntšoa ho lokela ho etsoa ho latela litaelo, 'me bophahamo ba sampole e ntšitsoeng ke 200μL 'me bophahamo bo khothaletsoang ba elution ke 80μL.


  • E fetileng:
  • E 'ngoe:

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