Chlamydia Trachomatis e omisitsoeng ka serame
Lebitso la sehlahisoa
HWTS-UR032C/D-Kit ea ho Fumana Chlamydia Trachomatis Nucleic Acid e omisitsoeng ka leqhoa (Enzymatic Probe Isothermal Amplification)
Thuto ea mafu a seoa
Chlamydia trachomatis (CT) ke mofuta oa likokoana-hloko tsa prokaryotic tse phelang ka har'a lisele tsa eukaryotic feela.[1]Chlamydia trachomatis e arotsoe ka li-serotype tsa AK ho latela mokhoa oa serotype. Litšoaetso tsa tsamaiso ea urogenital li bakoa haholo ke li-serotype tsa DK tse fapaneng tsa trachoma, 'me banna ba iponahatsa haholo e le urethritis, e ka phekoloang ntle le kalafo, empa boholo ba tsona li fetoha tse sa foleng, li mpefala nako le nako, 'me li ka kopanngoa le epididymitis, proctitis, jj.[2]. Tse tšehali li ka bakoa ke urethritis, cervicitis, jj., le mathata a tebileng a salpingitis.[3].
Mokero
| FAM | Chlamydia trachomatis (CT) |
| ROX | Taolo ea Ka Hare |
Li-parameter tsa Tekheniki
| Polokelo | ≤30℃ |
| Nako ea ho phela | Likhoeli tse 12 |
| Mofuta oa Sampole | Sesepa sa molomo oa popelo sa basali Sesepa sa urethra sa banna Moroto oa banna |
| Tt | ≤28 |
| CV | ≤10.0% |
| LoD | Likopi tse 400/mL |
| Ho hlaka | Ha ho na karabelo e kopaneng pakeng tsa kit ena le likokoana-hloko tse ling tsa tšoaetso ea genitourinary tract tse kang mofuta oa 16 oa Human papillomavirus o kotsi haholo, mofuta oa 18 oa Human papillomavirus, mofuta oa Ⅱ oa vaerase ea Herpes simplex, Treponema pallidum, Ureaplasma urealyticum, Mycoplasma hominis, Mycoplasma genitalium, Staphylococcus epidermidis, Escherichia coli, Gardnerella vaginalis, Candida albicans, Trichomonas vaginalis, Lactobacillus crispatus, Adenovirus, Cytomegalovirus, Beta Streptococcus, Vaerase ea immunodeficiency ea motho, Lactobacillus casei le DNA ea genomic ea motho, jj. |
| Lisebelisoa tse Sebetsang | Sistimi ea PCR ea Nako ea 'Nete e Sebelisitsoeng ea 7500 Mekhoa ea PCR e Potlakileng ea Li-Biosystem tse 7500 tsa Nako ea 'Nete QuantStudio®Mekhoa e 5 ea PCR ea Nako ea Sebele Mekhoa ea PCR ea Nako ea Sebele ea SLAN-96P (Hongshi Medical Technology Co., Ltd.) Leseli la ho Tsamaisa Li-Cycler®Sistimi ea PCR ea Nako ea 'Nete ea 480 Sistimi ea ho lemoha PCR ea LineGene 9600 Plus ea Nako ea 'Nete (FQD-96A, theknoloji ea Hangzhou Bioer) MA-6000 Nako ea Sebele ea Bongata ba Thermal Cycler Sistimi ea PCR ea BioRad CFX96 ea Nako ea 'Nete le Sistimi ea PCR ea Nako ea 'Nete ea BioRad CFX Opus 96 Sistimi e bonolo ea ho lemoha Fluorescence Isothermal ea nako ea sebele ea Amp()HWTS-1600). |
Phallo ea Mosebetsi
Khetho ea 1.
Setlolo sa ho Lokolla Sample Release Reagent sa Macro & Micro-Test (HWTS-3005-8). Ho ntšoa ho lokela ho etsoa ho latela IFU ka tieo. Kenya DNA ea sampole e ntšitsoeng ke setlolo sa ho lokolla sampole ka har'a buffer ea karabelo 'me u leke sesebelisoa ka kotloloho, kapa lisampole tse ntšitsoeng li lokela ho bolokoa mochesong oa 2-8℃ nako e sa feteng lihora tse 24.
Khetho ea 2.
Kit ea DNA/RNA e Akaretsang ea Macro le Micro-Test (HWTS-301)7-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B). Ho ntšoa ho lokela ho etsoa ho latela IFU ka tieo, 'me bophahamo bo khothaletsoang ba ho tlosa ke 80μL. DNA ea sampole e ntšoang ka mokhoa oa makenete e futhumatsoa ho 95°C metsotso e 3 ebe e hlatsuoa hang-hang ka leqhoa metsotso e 2. Kenya DNA ea sampole e sebetsitsoeng ka har'a buffer ea karabelo 'me teko sesebelisoa kapa lisampoleng tse sebetsitsoeng e lokela ho bolokoa ka tlase ho -18°C nako e sa feteng likhoeli tse 4. Palo ea ho hoamisa le ho qhibiliha ho pheta-phetoang ha ea lokela ho feta lipotoloho tse 4.
