Mefuta e 11 ea Likokoana-hloko tsa ho hema tse omisitsoeng ka leqhoa Nucleic Acid
Lebitso la sehlahisoa
HWTS-RT190 -E omisitsoe ka ho hatsela Mefuta e 11 ea Sete ea ho Fumana Likokoana-hloko tsa ho Phefumoloha ea Nucleic Acid (Fluorescence PCR)
Thuto ea mafu a seoa
Tšoaetso ea phefumoloho ke lefu la bohlokoa le behang bophelo bo botle ba batho kotsing e kholo. Lithuto li bontšitse hore boholo ba tšoaetso ea phefumoloho e bakoa ke likokoana-hloko tsa baktheria le/kapa tsa vaerase tse tšoaetsang moamoheli hammoho, e leng se lebisang ho matla a eketsehileng a lefu lena kapa esita le lefu. Ka hona, ho khetholla pathogen ho ka fana ka kalafo e reretsoeng le ho ntlafatsa sekhahla sa ho phela ha mokuli [1,2]. Leha ho le joalo, mekhoa ea setso ea ho lemoha likokoana-hloko tsa phefumoloho e kenyelletsa tlhahlobo ea microscope, setso sa baktheria le tlhahlobo ea sesole sa 'mele. Mekhoa ena e rarahane, e nka nako e telele, e hloka botekgeniki, 'me e na le kutlo e tlase. Ho feta moo, ha e khone ho lemoha likokoana-hloko tse ngata sampole e le 'ngoe, e leng se etsang hore ho be thata ho fa lingaka tlhahlobo e nepahetseng ea thuso. Ka lebaka leo, meriana e mengata e ntse e le mohatong oa meriana ea boiphihlelo, e seng feela e potlakisang potoloho ea ho hanyetsa baktheria, empa hape e ama tlhahlobo ea bakuli ka nako e loketseng [3]. Haemophilus influenzae e tloaelehileng, Acinetobacter baumannii, Pseudomonas aeruginosa, Klebsiella pneumoniae, Streptococcus pneumoniae, Stenotrophomonas maltophilia, Bordetella pertussis, Bordetella parapertussis, Mycoplasma pneumoniae, Chlamydia pneumoniae, le Legionella pneumophila ke likokoana-hloko tsa bohlokoa tse bakang tšoaetso ea nosocomial tract respiratory tract[4,5]. Kit ena ea teko e lemoha le ho khetholla nucleic acid e itseng ea likokoana-hloko tse boletsoeng ka holimo ho batho ba nang le matšoao le matšoao a tšoaetso ea phefumoloho, 'me e e kopanya le liphetho tse ling tsa laboratori ho thusa ho fumana tšoaetso ea phefumoloho.
Li-parameter tsa Tekheniki
| Polokelo | 2-30℃ |
| Nako ea ho phela | Likhoeli tse 12 |
| Mofuta oa Sampole | Sesepa sa 'metso |
| Ct | ≤33 |
| CV | <5.0% |
| LoD | LoD ea kit bakeng sa Klebsiella pneumoniae ke 500 CFU/mL; LoD ea Streptococcus pneumoniae ke 500 CFU/mL; LoD ea Haemophilus influenzae ke 1000 CFU/mL; LoD ea Pseudomonas aeruginosa ke 500 CFU/mL; LoD ea Acinetobacter baumannii ke 500 CFU/mL; LoD ea Stenotrophomonas maltophilia ke 1000 CFU/mL; LoD ea Bordetella pertussis ke 500 CFU/mL; LoD ea Bordetella parapertussis ke 500 CFU/mL; LoD ea Mycoplasma pneumoniae ke likopi tse 200/mL; LoD ea Legionella pneumophila ke 1000 CFU/mL; LoD ea Chlamydia pneumoniae ke likopi tse 200/mL. |
| Ho hlaka | Ha ho na karabelo e kopaneng pakeng tsa kit le dikokwana-hloko tse ding tse tlwaelehileng tsa ho hema ka ntle ho sebaka sa ho lemoha sa kit ya teko, mohlala, Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Serratia marcescens, Enterococcus faecalis, Candida albicans, Klebsiella oxytoca, Streptococcus pyogenes, Micrococcus luteus, Rhodococcus equi, Listeria monocytogenes, Acinetobacter junii, Haemophilus parainfluenzae, Legionella dumov, Enterobacter aerogenes, Haemophilus haemolyticus, Streptococcus salivarius, Neisseria meningitidis, Mycobacterium tuberculosis, Influenza A virus, Influenza B virus, Aspergillus flavus, Aspergillus terreus, Aspergillus fumigatus, Candida glabrata, le Candida tropicalis. |
| Lisebelisoa tse Sebetsang | Mofuta oa I: Lisebelisoa tsa Biosystems tse Sebelisitsoeng 7500 Mekhoa ea PCR ea Nako ea 'Nete, Lisebelisoa tsa Biosystems tse Sebelisitsoeng 7500 Mekhoa ea PCR e Potlakileng ea Nako ea 'Nete, QuantStudio®Mekhoa e 5 ea PCR ea Nako ea 'Nete, Mekhoa ea PCR ea Nako ea 'Nete ea SLAN-96P (Hongshi Medical Technology Co., Ltd.), LightCycler®480 Mekhoa ea PCR ea Nako ea 'Nete, LineGene 9600 Plus Mekhoa ea ho Fumana PCR ea Nako ea 'Nete (FQD-96A, theknoloji ea Hangzhou Bioer), MA-6000 Sesebelisoa sa ho Fuputsa Thermal sa Nako ea 'Nete (Suzhou Molarray Co., Ltd.), Sistimi ea PCR ea BioRad CFX96 ea Nako ea 'Nete, Sistimi ea PCR ea BioRad CFX Opus 96 ea Nako ea 'Nete. Mofuta oa II: EudemonTMAIO800 (HWTS-EQ007) ke Jiangsu Macro & Micro-Test Med-Tech Co. Ltd. |
Phallo ea Mosebetsi
Mofuta oa I: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (e ka sebelisoang le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) ea Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. li khothaletsoa bakeng sa ho ntša sampole 'me mehato e latelang e lokela ho etsoa ho latela IFU ea Kit.
