E omisitsoeng ka leqhoa Mefuta e 11 ea Likokoana-hloko Tse Phefumolohang Nucleic Acid
Lebitso la sehlahisoa
HWTS-RT190 -Ho Omisa-Ho Omisa-Ho Omisa-Mefuta e 11 ea Likokoana-hloko Tse Phefumolohang Nucleic Acid Detection Kit(Fluorescence PCR)
Epidemiology
Tšoaetso ea tsamaiso ea ho hema ke lefu la bohlokoa le sokelang bophelo ba batho ka ho teba. Liphuputso li bontšitse hore mafu a mangata a phefumoloho a bakoa ke likokoana-hloko tsa baktheria le/kapa kokoana-hloko tse tšoaetsanoang le moeti, e leng se lebisang ho matla a eketsehileng a lefu lena kapa esita le lefu. Ka hona, ho khetholla pathogen ho ka fana ka phekolo e lebisitsoeng le ho ntlafatsa lebelo la ho phela ha mokuli [1,2]. Leha ho le joalo, mekhoa ea setso ea ho lemoha likokoana-hloko tsa phefumoloho e kenyelletsa tlhahlobo ea microscopic, setso sa baktheria le tlhahlobo ea immunology. Mekhoa ena e rarahane, e ja nako, e hloka tsebo, ebile e na le kutlo e tlase. Ho phaella moo, ha ba khone ho lemoha likokoana-hloko tse ngata ka sampuli e le 'ngoe, e leng ho etsang hore ho be thata ho fa lingaka tlhahlobo e nepahetseng e thusang. Ka lebaka leo, meriana e mengata e ntse e le boemong ba meriana e matla, e sa potlakiseng feela potoloho ea ho hanyetsa baktheria, empa hape e ama ho hlahlojoa ka nako ea bakuli[3]. Common Haemophilus influenzae, Acinetobacter baumannii, Pseudomonas aeruginosa, Klebsiella pneumoniae, Streptococcus pneumoniae, Stenotrophomonas maltophilia, Bordetella pertussis, Bordetella parapertussis, Mycoplasma pneumoniae, Chlamydia pneumonia e bakang Chlamydia palamoone, Chlamydia pneumonia e bohlokoa mafu a matšoafo a nosocomial [4,5]. Setsi sena sa liteko se lemoha le ho khetholla li-nucleic acid tsa likokoana-hloko tse ka holimo ho batho ba nang le matšoao le matšoao a tšoaetso ea ho hema, 'me e li kopanya le liphello tse ling tsa laboratori ho thusa ho fumana tšoaetso ea kokoana-hloko ea matšoafo.
Tekheniki Parameters
| Polokelo | 2-30 ℃ |
| Shelf-bophelo | Likhoeli tse 12 |
| Mofuta oa Mohlala | Ho phunya 'metso |
| Ct | ≤33 |
| CV | <5.0% |
| LoD | LoD ea kit bakeng sa Klebsiella pneumoniae ke 500 CFU/mL; LoD ea Streptococcus pneumoniae ke 500 CFU/mL; LOD ea Haemophilus influenzae ke 1000 CFU/mL; LOD ea Pseudomonas aeruginosa ke 500 CFU/mL; LOD ea Acinetobacter baumannii ke 500 CFU/mL; LOD ea Stenotrophomonas maltophilia ke 1000 CFU/mL; LoD ea Bordetella pertussis ke 500 CFU / mL; LoD ea Bordetella parapertussis ke 500 CFU / mL; LOD ea Mycoplasma pneumoniae ke likopi tse 200/mL; LoD ea Legionella pneumophila ke 1000 CFU/mL; LOD ea Chlamydia pneumoniae ke likopi tse 200/mL. |
| Tse khethehileng | Ha ho na karohano lipakeng tsa kit le likokoana-hloko tse ling tse tloaelehileng tsa phefumoloho kantle ho sebaka sa tlhahlobo ea lisebelisoa, mohlala, Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Serratia marcescens, Enterococcus faecalis, Candida albicans, Klebsiella oxytoccus, Rhodococcus equi, Listeria monocytogenes, Acinetobacter junii, Haemophilus parainfluenzae, Legionella dumov, Enterobacter aerogenes, Haemophilus haemolyticus, Streptococcus salivarius, Neisseria meningitidis, Mycobacterium tuberculosis, kokoana-hloko ea Influespernza, Influesperanza Aspergillus terreus, Aspergillus fumigatus, Candida glabrata, le Candida tropicalis. |
| Lisebelisoa tse sebetsang | Mofuta oa I: Applied Biosystems 7500 Real-Time PCR Systems, Applied Biosystems 7500 Fast Real-Time PCR Systems, QuantStudio®Li-PCR Systems tse 5 tsa Nako ea 'Nete, SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.), LightCycler®480 Real-Time PCR Systems, LineGene 9600 Plus Real-Time PCR Detection Systems (FQD-96A, Hangzhou Bioer technology), MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.), BioRad CFX96 Real-Time PCR System-Biopus PCR System OTime9 Mofuta oa II: EudemonTMAIO800 (HWTS-EQ007) ka Jiangsu Macro & Micro-Test Med-Tech Co. Ltd. |
Phallo ea Mosebetsi
Mofuta oa I: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (e ka sebelisoang le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) ka Jiangsu Macro & Micro-Test Test Med-Tech Co., Ltd. ho latela IFU ea Kit.
