Vaerase ea Encephalitis ea Moru
Lebitso la sehlahisoa
Sete ea ho Fumana Sefuba sa Moru sa HWTS-FE006 sa Virusi ea Nucleic Acid (Fluorescence PCR)
Thuto ea mafu a seoa
Bolwetse ba moru (FE), bo tsejoang hape e le bolwetse ba moru bo bakwang ke matswele (TBE), ke lefu le tshwaetsanang le matla la tsamaiso ya methapo e bohareng le bakwang ke vaerase ya moru. Balwetse ba moru bo bakwang ke mofuta wa Flavivirus wa lelapa la Flaviviridae. Dikaroloana tsa balwetse di chitja ka bophara ba 40-50nm. Boima ba dimolekhule ke hoo e ka bang 4×106Da, 'me genome ea vaerase ke RNA e nang le moelelo o motle, e nang le thapo e le 'ngoe.[1]. Ka bongaka, e khetholloa ka feberu e phahameng, hlooho e opang, koma, ho qala ka potlako ho teneha ha meningeal, le ho holofala ha mesifa ea molala le maoto, 'me e na le sekhahla se phahameng sa lefu. Tlhahlobo ea pele le e potlakileng ea kokoana-hloko ea encephalitis ea meru ke senotlolo sa kalafo ea encephalitis ea meru, 'me ho thehoa ha mokhoa o bonolo, o tobileng le o potlakileng oa tlhahlobo ea etiological ke habohlokoa haholo tlhahlobong ea bongaka ea encephalitis ea meru.[1,2].
Mokero
| FAM | asiti ea nucleic acid ea kokoana-hloko ea encephalitis ea meru |
| ROX | Taolo ea Ka Hare |
Li-parameter tsa Tekheniki
| Polokelo | ≤-18℃ |
| Nako ea ho phela | Likhoeli tse 9 |
| Mofuta oa Sampole | serum e foreshe |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | Likopi tse 500/mL |
| Lisebelisoa tse Sebetsang | Mekhoa ea Li-biosystem tse Sebelisitsoeng 7500 Mekhoa ea PCR ea Nako ea Sebele Mekhoa ea PCR e Potlakileng ea Li-Biosystem tse 7500 tsa Nako ea 'Nete QuantStudio®Mekhoa e 5 ea PCR ea Nako ea Sebele Mekhoa ea PCR ea Nako ea Sebele ea SLAN-96P Leseli la ho Tsamaisa Li-Cycler®Sistimi ea PCR ea Nako ea 'Nete ea 480 Litsamaiso tsa ho lemoha PCR tsa LineGene 9600 Plus tsa Nako ea 'Nete MA-6000 Nako ea Sebele ea Bongata ba Thermal Cycler Sistimi ea PCR ea BioRad CFX96 ea Nako ea 'Nete Sistimi ea PCR ea BioRad CFX Opus 96 ea Nako ea 'Nete |
Phallo ea Mosebetsi
Reagent e kgothalletswang ho ntsha: Nucleic Acid Extraction kapa Purification Kit (YDP315-R) ke Tiangen Biotech(Beijing) Co.,Ltd., ho ntsha ho lokela ho etswa ho ya ka ditaelo ka tieo. Bophahamo ba sampole e kgothalletswang ho ntshwa ke 140μL mme bophahamo ba elution e kgothalletswang ke 60μL.
Reagent e kgothalletswang ho ntsha: Kit ya Macro & Micro-Test General DNA/RNA (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006, HWTS-3006C, HWTS-3006B). ho ntsha ho lokela ho etswa ho ya ka ditaelo ka tieo. Bophahamo ba sampole e kgothaletswang ke 200μL mme bophahamo ba elution e kgothaletswang ke 80μL.
