Virusi ea Forest Encephalitis
Lebitso la sehlahisoa
HWTS-FE006 Forest Encephalitis Virus Nucleic Acid Detection Kit(Fluorescence PCR)
Epidemiology
Forest encephalitis (FE), eo hape e tsejoang e le tick-borne encephalitis (Tick-borne encephalitis, TBE), ke lefu le tšoaetsanoang le tebileng la tsamaiso ea methapo e bohareng le bakoang ke kokoana-hloko ea morung ea encephalitis. Virusi ea Forest encephalitis ke ea mofuta oa Flavivirus oa lelapa la Flaviviridae. Likaroloana tsa vaerase li chitja ka bophara ba 40-50nm. Boima ba molek'hule ke hoo e ka bang 4 × 106Da, le genome ea kokoana-hloko ke RNA e nang le thapo e le 'ngoe[1]. Ho ea ka meriana, e khetholloa ke feberu e matla, hlooho e opang, koma, ho ferekana ha meningeal ka potlako, le ho holofala ha mesifa ea molala le maoto le matsoho, 'me ho na le sekhahla se phahameng sa ho shoa. Tlhahlobo ea pele le e potlakileng ea kokoana-hloko ea meru ea encephalitis ke senotlolo sa phekolo ea encephalitis ea morung, 'me ho thehoa ha mokhoa o bonolo, o tobileng le o potlakileng oa ho hlahloba likokoana-hloko ke oa bohlokoa haholo tlhahlobong ea bongaka ea encephalitis ea meru.[1,2].
Channel
| FAM | morung encephalitis kokoana-hloko nucleic acid |
| ROX | Taolo ea ka hare |
Tekheniki Parameters
| Polokelo | ≤-18℃ |
| Shelf-bophelo | Likhoeli tse 9 |
| Mofuta oa Mohlala | serum e ncha |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | 500Likopi / mL |
| Lisebelisoa tse sebetsang | Applied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®Litsamaiso tse 5 tsa nako ea 'nete tsa PCR Litsamaiso tsa PCR tsa nako ea sebele tsa SLAN-96P LightCycler®Sistimi ea 480 ea Nako ea 'Nete ea PCR LineGene 9600 Plus Nako ea 'Nete ea PCR ea ho Khetholla Sistimi MA-6000 Real-Nako Quantitative Thermal Cycler Sistimi ea PCR ea Nako ea 'Nete ea BioRad CFX96 BioRad CFX Opus 96 Real-Nako PCR System |
Phallo ea Mosebetsi
Sehlahisoa se khothalelitsoeng sa ho ntšoa: Nucleic Acid Extraction or Purification Kit (YDP315-R) ke Tiangen Biotech(Beijing) Co.,Ltd., ho ntšoa ho lokela ho etsoa ho latela litaelo tse tiileng. Bolumo ea sampole e khothalelitsoeng ke 140μL mme bophahamo ba molumo o khothaletsoang ke 60μL.
Sehlahisoa se khothalelitsoeng sa ho ntša: Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-06060) HWTS-3006B). ho ntšoa ho lokela ho etsoa ho ea ka litaelo ka tieo. Bolumo ea sampole e khothalelitsoeng ke 200μL mme bophahamo ba molumo o khothaletsoang ke 80μL.
