Vaerase ea Dengue, Vaerase ea Zika le Vaerase e ngata ea Chikungunya
Lebitso la sehlahisoa
Kiti ea ho lemoha Vaerase ea Dengue ea HWTS-FE040, Vaerase ea Zika le Chikungunya Virus Multiplex Nucleic Acid (Fluorescence PCR)
Thuto ea mafu a seoa
Feberu ea Dengue (DF), e bakoang ke tšoaetso ea kokoana-hloko ea dengue (DENV), ke e 'ngoe ea mafu a tšoaetsanoang a arbovirus a seoa ka ho fetisisa. Phetiso ea eona e kenyelletsa Aedes aegypti le Aedes albopictus. DF e atile haholo libakeng tsa tropike le tsa subtropic. DENV ke ea flavivirus tlas'a flaviviridae, 'me e ka aroloa ka li-serotype tse 4 ho latela antigen ea holim'a metsi. Lipontšo tsa kliniki tsa tšoaetso ea DENV li kenyelletsa hlooho e opang, feberu, bofokoli, ho hola ha lymph node, leukopenia le jj., le ho tsoa mali, ho tšoha, kotsi ea sebete kapa esita le lefu maemong a boima. Lilemong tsa morao tjena, phetoho ea tlelaemete, ho hola ha litoropo, nts'etsopele e potlakileng ea bohahlauli le lintlha tse ling li fane ka maemo a potlakileng le a bonolo a ho fetisa le ho ata ha DF, e leng se lebisang katolosong e sa khaotseng ea sebaka sa seoa sa DF.
Mokero
| FAM | Asiti ea nucleic ea DENV |
| ROX | Taolo ea Ka Hare |
Li-parameter tsa Tekheniki
| Polokelo | -18℃ |
| Nako ea ho phela | Likhoeli tse 9 |
| Mofuta oa Sampole | Serum e ncha |
| Ct | ≤38 |
| CV | ＜5% |
| LoD | Likopi tse 500/mL |
| Ho hlaka | Liphetho tsa liteko tsa tšitiso li bontša hore ha mahloriso a bilirubin ka har'a serum a sa fete 168.2μmol/ml, mahloriso a hemoglobin a hlahisoang ke hemolysis ha a fete 130g/L, mahloriso a lipid maling a sa fete 65mmol/ml, mahloriso a IgG ka kakaretso ka har'a serum ha a fete 5mg/mL, ha ho na phello ho kokoana-hloko ea dengue, kokoana-hloko ea Zika kapa chikungunya. Kokoana-hloko ea Hepatitis A, kokoana-hloko ea Hepatitis B, kokoana-hloko ea Hepatitis C, kokoana-hloko ea Herpes, kokoana-hloko ea Eastern equine encephalitis, Hantavirus, kokoana-hloko ea Bunya, kokoana-hloko ea West Nile le lisampole tsa serum ea genomic ea batho li khethiloe bakeng sa teko ea ho kopanya lisele, 'me liphetho li bontša hore ha ho na karabelo e kopaneng pakeng tsa kit ena le likokoana-hloko tse boletsoeng ka holimo. |
| Lisebelisoa tse Sebetsang | Sistimi ea PCR ea Nako ea 'Nete e Sebelisitsoeng ea 7500 Mekhoa ea PCR e Potlakileng ea Li-Biosystem tse 7500 tsa Nako ea 'Nete QuantStudio®Mekhoa e 5 ea PCR ea Nako ea Sebele Leseli la ho Tsamaisa Li-Cycler®Sistimi ea PCR ea Nako ea 'Nete ea 480 Sistimi ea ho lemoha PCR ea LineGene 9600 Plus ea Nako ea 'Nete MA-6000 Nako ea Sebele ea Bongata ba Thermal Cycler Sistimi ea PCR ea BioRad CFX96 ea Nako ea 'Nete Sistimi ea PCR ea BioRad CFX Opus 96 ea Nako ea 'Nete |
Phallo ea Mosebetsi
Khetho ea 1.
TIANamp Virus DNA/RNA Kit (YDP315-R), 'me ho ntšoa ha eona ho lokela ho etsoa ho latela litaelo tsa tšebeliso. Bophahamo ba sampole e ntšitsoeng ke 140μL, 'me bophahamo bo khothaletsoang ba ho hlapolla ke 60μL.
Khetho ea 2.
Seteishene sa DNA/RNA se Akaretsang sa Macro le Micro-Test (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (se ka sebediswang le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) ke Jiangsu Macro & Micro-Test Med-Tech Co., Ltd., mme ho ntshwa ha yona ho lokela ho etswa ho ya ka ditaelo tsa tshebediso. Bophahamo ba sampole e ntshitsweng ke 200μL, mme bophahamo bo kgothaletswang ba ho hlakisa ke 80μL.
