Kokoana ea Dengue, Zika Virus le Chikungunya Virus Multiplex
Lebitso la sehlahisoa
HWTS-FE040 Virus ea Dengue, Zika Virus le Chikungunya Virus Multiplex Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Dengue fever (DF), e bakoang ke tšoaetso ea kokoana-hloko ea dengue (DENV), ke e 'ngoe ea mafu a tšoaetsanoang ka ho fetisisa a arbovirus. Sebaka sa eona sa phetisetso se kenyelletsa Aedes aegypti le Aedes albopictus. DF e atile haholo libakeng tsa tropike le tsa tropike. DENV ke ea flavivirus tlas'a flaviviridae, 'me e ka aroloa ka 4 serotypes ho ea ka holim'a antigen. Lipontšo tsa bongaka tsa tšoaetso ea DENV haholo-holo li kenyelletsa hlooho, feberu, bofokoli, ho atolosoa ha lymph node, leukopenia joalo-joalo, le ho tsoa mali, ho tšoha, kotsi ea sebete kapa esita le lefu maemong a matla. Lilemong tsa morao tjena, phetoho ea maemo a leholimo, ho ata ha litoropo, nts'etsopele e potlakileng ea bohahlauli le lintlha tse ling li fane ka maemo a potlakileng le a bonolo a ho fetisa le ho hasana ha DF, e leng se lebisang keketsehong e sa khaotseng ea sebaka sa seoa sa DF.
Channel
| FAM | DENV nucleic acid |
| ROX | Taolo ea ka hare |
Tekheniki Parameters
| Polokelo | -18 ℃ |
| Shelf-bophelo | Likhoeli tse 9 |
| Mofuta oa Mohlala | Serum e ncha |
| Ct | ≤38 |
| CV | ＜5% |
| LoD | 500 Likopi / mL |
| Tse khethehileng | Liphetho tsa liteko tsa tšitiso li bonts'a hore ha khatello ea bilirubin ka serum e sa fete 168.2μmol/ml, khatello ea hemoglobin e hlahisoang ke hemolysis ha e fete 130g/L, khatello ea lipid maling ha e fete 65mmol/ml, kakaretso ea IgG ka serum ha e fete 5mg/mL, ha ho na kokoana-hloko ea dengue kapa kokoana-hloko ea Zika. Kokoana ea Hepatitis A, kokoana-hloko ea Hepatitis B, kokoana-hloko ea Hepatitis C, kokoana-hloko ea Herpes, kokoana-hloko ea Eastern equine encephalitis, Hantavirus, kokoana-hloko ea Bunya, kokoana-hloko ea West Nile le lisampole tsa batho ba genomic serum li khethoa bakeng sa tlhahlobo ea cross-reactivity, 'me liphetho li bontša hore ha ho na karabelo ea sefapano pakeng tsa kit ena le likokoana-hloko tse boletsoeng ka holimo. |
| Lisebelisoa tse sebetsang | Lisebelisoa tsa Biosystems 7500 tsa Nako ea 'Nete ea PCR Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®Litsamaiso tse 5 tsa nako ea 'nete tsa PCR LightCycler®Sistimi ea 480 ea Nako ea 'Nete ea PCR LineGene 9600 Plus Nako ea 'Nete ea PCR ea ho Fumana Sistimi MA-6000 Real-Nako Quantitative Thermal Cycler Sistimi ea PCR ea Nako ea 'Nete ea BioRad CFX96 BioRad CFX Opus 96 Real-Nako PCR System |
Phallo ea Mosebetsi
Khetho ea 1.
TIANMP Virus DNA/RNA Kit (YDP315-R), 'me ho ntšoa ho lokela ho etsoa ho latela litaelo tsa tšebeliso. Bolumo ea sampole e ntšitsoeng ke 140μL, 'me molumo o khothaletsoang oa elution ke 60μL.
Khetho ea 2.
Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (e ka sebelisoang le Macro & Micro-Test Automatic Nucleic Acid Extractor (HCTS0, HWTS-30B) & Micro-Test Med-Tech Co., Ltd., 'me ho ntšoa ho lokela ho etsoa ho latela taelo ea tšebeliso. Bolumo ea sampole e ntšitsoeng ke 200μL, 'me molumo o khothaletsoang oa elution ke 80μL.
