Mofuta oa Coxsackie Virus A16 Nucleic Acid

Tlhaloso e Khutšoanyane:

Setsi sena se sebelisetsoa ho lemoha boleng ba in vitro kokoana-hloko ea Coxsackie A16 nucleic acid ka har'a 'metso oa motho.


Lintlha tsa Sehlahisoa

Li-tag tsa Sehlahisoa

Lebitso la sehlahisoa

HWTS-EV025-Coxsackie Virus Mofuta oa A16 Nucleic Acid Detection Kit (Enzymatic Probe Isothermal Amplification)

Setifikeiti

CE

Epidemiology

Setsi sena se nka Enzymatic Probe Isothermal Amplification (EPIA), 'me se etsa li-primers tse khethehileng le lisebelisoa tse nang le motheo tsa RNA (rProbe) bakeng sa sebaka se sirelelitsoeng haholo sa Cox A16,' me se eketsa enzyme ea Bst le enzyme ea RNaseH ka nako e le 'ngoe, moo ho le letšehali le lipheletsong tse ka ho le letona tsa motheo oa RNA oa rProbe li ngotsoe ka lihlopha tsa fluorescent le quencher, ka ho latellana.Sebelisa ts'ebetso ea DNA polymerase le tšebetso ea strand displacement ea enzyme ea Bst ho holisa sepheo sa ho lekoa ka mocheso o sa fetoheng, enzyme ea RNaseH e ka qhekella metheo ea RNA holim'a ketane e nyalisitsoeng ea target-probe, e le hore sehlopha sa fluorescent le quencher ea rProbe li khonehe. e arohaneng ka tsela eo fluorescing.Ho feta moo, sekhechana se ka letsohong le letšehali la setsi sa masala sa rProbe RNA se ka sebelisoa e le setsi sa pele sa ho atolosa ho etsa sehlahisoa, se bokellang sehlahisoa.Letšoao la fluorescent le ntse le bokelloa ka mokhoa o tsoelang pele ha ho etsoa sehlahisoa, ka hona ho lemoha ho fumanoa ha sepheo sa nucleic acid.

Channel

FAM Mofuta oa kokoana-hloko ea Coxsackie A16
ROX taolo ea ka hare

Tekheniki Parameters

Polokelo

≤-18℃

Shelf-bophelo

Likhoeli tse 12

Mofuta oa Mohlala

Li-swabs tsa 'metso tse sa tsoa bokelloa

CV

≤10.0%

Ct

≤38

LoD

2000Likopi/mL

Lisebelisoa tse sebetsang

Applied Biosystems 7500 Real-Time PCR SystemsLitsamaiso tsa PCR tsa nako ea sebele tsa SLAN-96P

Sesebelisoa sa PCR sa LightCycler®480 Real-Time

Mokhoa oa 'nete oa ho lemoha mocheso oa nako ea 'nete oa Fluorescence Constant Temperature System Easy Amp HWTS1600

Phallo ea Mosebetsi

Khetho ea 1

Sehlahisoa se khothalelitsoeng sa ho ntša: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3001, HWTS-3004-32, HWTS-3004-48) le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006)

Khetho ea 2

Sehlahisoa sa ho ntša se khothalelitsoeng: Macro & Micro-Test Sample Release Reagent (HWTS-3005-8).


  • E fetileng:
  • E 'ngoe:

  • Ngola molaetsa wa hao mona mme o re romele wona