Mofuta oa Coxsackie Virus A16 Nucleic Acid
Lebitso la sehlahisoa
HWTS-EV025-Coxsackie Virus Mofuta oa A16 Nucleic Acid Detection Kit (Enzymatic Probe Isothermal Amplification)
Setifikeiti
CE
Epidemiology
Setsi sena se nka Enzymatic Probe Isothermal Amplification (EPIA), 'me se etsa li-primers tse khethehileng le lisebelisoa tse nang le motheo tsa RNA (rProbe) bakeng sa sebaka se sirelelitsoeng haholo sa Cox A16,' me se eketsa enzyme ea Bst le enzyme ea RNaseH ka nako e le 'ngoe, moo ho le letšehali le lipheletsong tse ka ho le letona tsa motheo oa RNA oa rProbe li ngotsoe ka lihlopha tsa fluorescent le quencher, ka ho latellana.Sebelisa ts'ebetso ea DNA polymerase le tšebetso ea strand displacement ea enzyme ea Bst ho holisa sepheo sa ho lekoa ka mocheso o sa fetoheng, enzyme ea RNaseH e ka qhekella metheo ea RNA holim'a ketane e nyalisitsoeng ea target-probe, e le hore sehlopha sa fluorescent le quencher ea rProbe li khonehe. e arohaneng ka tsela eo fluorescing.Ho feta moo, sekhechana se ka letsohong le letšehali la setsi sa masala sa rProbe RNA se ka sebelisoa e le setsi sa pele sa ho atolosa ho etsa sehlahisoa, se bokellang sehlahisoa.Letšoao la fluorescent le ntse le bokelloa ka mokhoa o tsoelang pele ha ho etsoa sehlahisoa, ka hona ho lemoha ho fumanoa ha sepheo sa nucleic acid.
Channel
FAM | Mofuta oa kokoana-hloko ea Coxsackie A16 |
ROX | taolo ea ka hare |
Tekheniki Parameters
Polokelo | ≤-18℃ |
Shelf-bophelo | Likhoeli tse 12 |
Mofuta oa Mohlala | Li-swabs tsa 'metso tse sa tsoa bokelloa |
CV | ≤10.0% |
Ct | ≤38 |
LoD | 2000Likopi/mL |
Lisebelisoa tse sebetsang | Applied Biosystems 7500 Real-Time PCR SystemsLitsamaiso tsa PCR tsa nako ea sebele tsa SLAN-96P Sesebelisoa sa PCR sa LightCycler®480 Real-Time Mokhoa oa 'nete oa ho lemoha mocheso oa nako ea 'nete oa Fluorescence Constant Temperature System Easy Amp HWTS1600 |
Phallo ea Mosebetsi
Khetho ea 1
Sehlahisoa se khothalelitsoeng sa ho ntša: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3001, HWTS-3004-32, HWTS-3004-48) le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006)
Khetho ea 2
Sehlahisoa sa ho ntša se khothalelitsoeng: Macro & Micro-Test Sample Release Reagent (HWTS-3005-8).