Chlamydia Trachomatis, Neisseria Gonorrhoeae le Trichomonas vaginalis
Lebitso la sehlahisoa
HWTS-UR041 Chlamydia Trachomatis, Neisseria Gonorrhoeae le Trichomonas vaginalis Nucleic Acid Detection Kit (Fluorescence PCR)
Thuto ea mafu a seoa
Chlamydia trachomatis (CT) ke mofuta oa likokoana-hloko tsa prokaryotic tse nang le likokoana-hloko liseleng tsa eukaryotic feela. Chlamydia trachomatis e arotsoe ka li-serotype tsa AK ho latela mokhoa oa serotype. Litšoaetso tsa mokhoa oa urogenital li bakoa haholo ke li-serotype tsa DK tse fapaneng tsa trachoma, 'me banna ba iponahatsa haholo e le urethritis, e ka fokotsoang ntle le kalafo, empa boholo ba tsona li fetoha tse sa foleng, li mpefala nako le nako, 'me li ka kopanngoa le epididymitis, proctitis, jj.
Mokero
| FAM | Chlamydia trachomatis |
| ROX | Neisseria gonorrhoeae |
| CY5 | Bofuma ba Trichomonal |
| VIC/HEX | Taolo ea Ka Hare |
Li-parameter tsa Tekheniki
| Polokelo | -18℃ |
| Nako ea ho phela | Likhoeli tse 12 |
| Mofuta oa Sampole | Swab ea molomo oa popelo ea mosali,Sesepa sa botšehali sa basali,Sesepa sa urethra sa banna |
| Ct | ≤38 |
| CV | <5% |
| LoD | 400Likopi/mL |
| Ho hlaka | Ha ho na karabelo e kopanang le dikokwana-hloko tse ding tsa tshwaetso ya STD ka ntle ho sebaka sa ho lemoha sa kit ya teko, jwalo ka Treponema pallidum, Mycoplasma hominis, Mycoplasma genitalium, Herpes simplex virus type 1, Herpes simplex virus type 2, Candida albicans, jj. |
| Lisebelisoa tse Sebetsang | Sistimi ea PCR ea Nako ea 'Nete e Sebelisitsoeng ea 7500 Mekhoa ea PCR e Potlakileng ea Li-Biosystem tse 7500 tsa Nako ea 'Nete QuantStudio®Mekhoa e 5 ea PCR ea Nako ea Sebele Mekhoa ea PCR ea Nako ea Sebele ea SLAN-96P Leseli la ho Tsamaisa Li-Cycler®Sistimi ea PCR ea Nako ea 'Nete ea 480 Sistimi ea ho lemoha PCR ea LineGene 9600 Plus ea Nako ea 'Nete MA-6000 Nako ea Sebele ea Bongata ba Thermal Cycler Sistimi ea PCR ea BioRad CFX96 ea Nako ea 'Nete Sistimi ea PCR ea BioRad CFX Opus 96 ea Nako ea 'Nete |
Phallo ea Mosebetsi
Khetho ea 1.
Reagent e kgothalletswang ya ho ntsha: Pipette 1mL ya sampole e tla lekoa ho 1.5mL ya tube ya centrifuge e se nang DNase/RNase, centrifuge ka 12000rpm metsotso e 3, lahla supernatant mme o boloke precipitate. Kenya 200µL ya saline e tlwaelehileng ho precipitate ho tswela pele ho tshwarwa. Macro & Micro-Test General DNA/RNA Kit (HWTS-3019-50, HWTS-3019-32, HWTS-3019-48, HWTS-3019-96) (e ka sebediswang le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) ke Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ho ntshwa ho lokela ho etswa ho ya ka ditaelo tsa tshebediso. Bophahamo ba sampole e ntshitsweng ke 200µL, mme bophahamo bo kgothaletswang ba elution ke 80µL.
Khetho ea 2.
Setlolo se kgothaletswang sa ho ntsha: Nucleic Acid Extraction kapa Purification Kit (YDP302). Ho ntsha ho lokela ho etswa ka thata ho ya ka ditaelo tsa tshebediso. Bophahamo bo kgothaletswang ba elution ke 80µL.
