Mefuta e 4 ea Likokoana-hloko tsa Phefumoloho
Lebitso la sehlahisoa
HWTS-RT099- Mefuta e 4 ea Likokoana-hloko tse Phefumolohang Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Lefu la Corona Virus 2019, le bitsoang "COVID-19", le bolela pneumonia e bakoang ke2019-nCoVtšoaetso.2019-nCoVke coronavirus ea mofuta oa β.COVID-19 ke lefu le tšoaetsanoang le matla la phefumoloho, 'me baahi ba bangata hangata ba kotsing.Hona joale, mohloli oa tšoaetso ke haholo-holo bakuli ba tšoaelitsoeng ke2019-nCoV, ’me batho ba tšoaelitsoeng ba se nang matšoao le bona ba ka ’na ba fetoha mohloli oa tšoaetso.Ho ipapisitsoe le lipatlisiso tsa morao-rao tsa epidemiological, nako ea ho incubation ke matsatsi a 1-14, haholo matsatsi a 3-7.Feberu, ho khohlela ho omileng le mokhathala ke lipontšo tse ka sehloohong.Bakuli ba seng bakae ba ne ba e-na le matšoaos joalo katšubuhlellano ea linko, nko e ntšang metsi, 'metso o bohloko, myalgia le letšollo, etc.
Channel
| FAM | 2019-nCoV |
| VIC(HEX) | RSV |
| CY5 | IFV A |
| ROX | IFV B |
| NED | Taolo ea ka hare |
Tekheniki Parameters
| Polokelo | -18℃ |
| Shelf-bophelo | Likhoeli tse 9 |
| Mofuta oa Mohlala | Oropharyngeal swab |
| Ct | ≤38 |
| LoD | 2019-nCoV: 300Copies/mLKokoana-hloko ea Influenza A/Influenza B/virus ea ho hema ea syncytial: 500Copies/mL |
| Tse khethehileng | a) Liphetho tsa li-cross-reactivity li bontša hore ha ho na karabelo e fapaneng lipakeng tsa kit le coronavirus ea motho SARSr-CoV, MERSr-CoV, HCoV-OC43, HCoV-229E, HCoV-HKU1, HCoV-NL63, parainfluenza virus type 1, 2, 3, rhinovirus A, B, C, chlamydia pneumoniae, metapneumovirus ea motho, enterovirus A, B, C, D, kokoana-hloko ea matšoafo ea motho, kokoana-hloko ea epstein-barr, kokoana ea maselese, kokoana-hloko ea cytomegalo, rotavirus, norovirus, kokoana-hloko ea parotitis, varicella-zoster kokoana-hloko, legionella, bordetella pertussis, haemophilus influenzae, staphylococcus aureus, streptococcus pneumoniae, streptococcus pyogenes, klebsiella pneumoniae, mycobacterium tuberculosis, mosi aspergillus, candida albicans, candida glabrata glabrata cystomic genomic gerobe, pnejinococcus genus cryptococcus, pnemycoccus, pnecoccus human, pnecoccus, pnecoccus, pneucoccus, pnecoccus, pneucoccus, pneucoccus, pnemycoccus, pneucoccus, pneucoccus, pnemycoccus, pnecoccus human, pnemycoccus le pneucoccus. b) Bokhoni ba ho thibela ho kena-kenana: khetha mucin (60mg/mL), 10% (v/v) ea mali le phenylephrine (2mg/mL), oxymetazoline (2mg/mL), sodium chloride (ho kenyeletsoa li-preservatives) (20 mg/mL ), beclomethasone (20mg/mL), dexamethasone (20mg/mL), flunisolide (20μg/mL), triamcinolone acetonide (2mg/mL), budesonide (2mg/mL), mometasone (2mg/mL), fluticasone (2mg/mL), ), histamine hydrochloride (5mg/mL), alpha interferon (800IU/mL), zanamivir (20mg/mL), ribavirin (10mg/mL), oseltamivir (60ng/mL), peramivir (1mg/mL), lopinavir (500mg/ mL), ritonavir (60mg/mL), mupirocin (20mg/mL), azithromycin (1mg/mL), ceftriaxone (40μg/mL), meropenem (200mg/mL), levofloxacin (10μg/mL) le tobramycin (0.6mg/ mL) bakeng sa tlhahlobo ea tšitiso, 'me liphetho li bonts'a hore lintho tse sitisang tse nang le likhakanyo tse boletsoeng ka holimo ha li na karabelo ea tšitiso ho liphetho tsa liteko tsa likokoana-hloko. |
| Lisebelisoa tse sebetsang | Lisebelisoa tsa Biosystems 7500 tsa Nako ea 'Nete ea PCR Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Nako ea 'Nete ea PCR Systems |
Phallo ea Mosebetsi
Khetho ea 1.
Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006) e hlahisoang ke Jiangsu Macro & Micro -Test Med-Tech Co., Ltd. Molumo oa sampole o ntšitsoeng ke 200μL, 'me molumo o khothaletsoang oa elution ke 80μL.
Khetho ea 2.
QIAamp Viral RNA Mini Kit (52904) e hlahisoang ke QIAGEN kapa Nucleic Acid Extraction or Purification Kit (YDP315-R) e hlahisoang ke Tiangen Biotech (Beijing) Co., Ltd. Molumo oa sampole o ntšitsoeng ke 140μL, 'me molumo oa elution o khothalletsoang ke 60μL.
