Mefuta e 4 ea Likokoana-hloko tsa Phefumoloho
Lebitso la sehlahisoa
HWTS-RT099- Mefuta e 4 ea Likokoana-hloko tse Phefumolohang Nucleic Acid Detection Kit (Fluorescence PCR)-内参是NED-ABI 7500 Real-Time PCR System/ABI 7500 Fast Real-Time PCR Systems/QuantStudio®Litsamaiso tse 5 tsa nako ea 'nete tsa PCR
HWTS-RT158-4 Mefuta ea Likokoana-hloko tse Phefumolohang Nucleic Acid Detection Kit (Fluorescence PCR)-Setšoantšo sa Quasar 705
Epidemiology
Lefu la Corona Virus 2019, le bitsoang "COVID-19", le bolela pneumonia e bakoang ke2019-nCoVtšoaetso.2019-nCoVke coronavirus ea mofuta oa β. COVID-19 ke lefu le tšoaetsanoang le matla la phefumoloho, 'me baahi ba bangata hangata ba kotsing. Hona joale, mohloli oa tšoaetso ke haholo-holo bakuli ba tšoaelitsoeng ke2019-nCoV, ’me batho ba tšoaelitsoeng ba se nang matšoao le bona ba ka ’na ba fetoha mohloli oa tšoaetso. Ho ipapisitsoe le lipatlisiso tsa morao-rao tsa epidemiological, nako ea ho incubation ke matsatsi a 1-14, haholo matsatsi a 3-7. Feberu, ho khohlela ho omileng le mokhathala ke lipontšo tse ka sehloohong. Bakuli ba seng bakae ba ne ba e-na le matšoaos joalo katšubuhlellano ea linko, nko e ntšang metsi, 'metso o bohloko, myalgia le letšollo, etc.
Channel
| FAM | 2019-nCoV |
| VIC(HEX) | RSV |
| CY5 | IFV A |
| ROX | IFV B |
| NED | Taolo ea ka hare |
Tekheniki Parameters
| Polokelo | -18℃ |
| Shelf-bophelo | Likhoeli tse 9 |
| Mofuta oa Mohlala | Oropharyngeal swab |
| Ct | ≤38 |
| LoD | 2019-nCoV: 300Copies/mLKokoana-hloko ea Influenza A/Kokoana-hloko ea Mofoka B/Vaerase ea ho hema ea syncytial: 500Copies/mL |
| Tse khethehileng | a) Liphetho tsa ts'ebetso e fapaneng li bonts'a hore ha ho na karabelo e fapaneng lipakeng tsa kit le coronavirus ea motho SARSr-CoV, MERSr-CoV, HCoV-OC43, HCoV-229E, HCoV-HKU1, HCoV-NL63, parainfluenza virus type 1, 2, 3, rhinovirus A, B, human pneumoniae, pneumoniae, C, pneumoniae, C, C A, B, C, D, kokoana-hloko ea pulmonary ea motho, kokoana-hloko ea epstein-barr, kokoana-hloko ea maselese, kokoana-hloko ea cytomegalo, rotavirus, norovirus, parotitis virus, varicella-zoster virus, legionella, bordetella pertussis, haemophilus influenzae, staphylococcus aureus, streptocusccus streptococcus streptococcus streptococcus streptococcus streptococcus pneumoniae, lefuba la mycobacterium, aspergillus ea mosi, candida albicans, candida glabrata, pneumocystis jiroveci le cryptococcus e sa tsoa tsoaloa le acid ea motho ea genomic nucleic. b) Bokhoni ba ho thibela ho kena-kenana: khetha mucin (60mg/mL), 10% (v/v) ea mali le phenylephrine (2mg/mL), oxymetazoline (2mg/mL), sodium chloride (ho kenyeletsoa li-preservatives) (20 mg/mL), beclomethasone (20mg/mL), dexametham/mL), dexamethasone (20μg/mL), triamcinolone acetonide (2mg/mL), budesonide (2mg/mL), mometasone (2mg/mL), fluticasone (2mg/mL), histamine hydrochloride (5mg/mL), alpha interferon (800IU/mL), zanamimvirim/mL), zanamimvirim/mL (10ba rimg rimg), osenamivir (20) (60ng/mL), peramivir (1mg/mL), lopinavir (500mg/mL), ritonavir (60mg/mL), mupirocin (20mg/mL), azithromycin (1mg/mL), ceftriaxone (40μg/mL), meropenem (200mg/mL), levofloxamy/mL), levofloxamy (0.6mg / mL) bakeng sa tlhahlobo ea tšitiso, 'me liphetho li bontša hore ho kena-kenana le lintho tse nang le likhahla tse boletsoeng ka holimo ha ho na ho kena-kenana le liphello tsa liteko tsa likokoana-hloko. |
| Lisebelisoa tse sebetsang | Lisebelisoa tsa Biosystems 7500 tsa Nako ea 'Nete ea PCRApplied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Nako ea 'Nete ea PCR Systems |
Phallo ea Mosebetsi
Khetho ea 1.
Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006) e hlahisoang ke Jiangsu Macro & Micro-Test extract. bophahamo ba modumo e kgothaletswang ke 80μL.
Khetho ea 2.
QIAamp Viral RNA Mini Kit (52904) e hlahisoang ke QIAGEN kapa Nucleic Acid Extraction or Purification Kit (YDP315-R) e hlahisoang ke Tiangen Biotech (Beijing) Co., Ltd. Molumo oa sampole o ntšitsoeng ke 140μL, 'me molumo oa elution o khothalletsoang ke 60μL.
