Mefuta e 29 ea Likokoana-hloko tsa ho Phefumoloha tse Kopantsoeng tsa Nucleic Acid
Lebitso la sehlahisoa
HWTS-RT160 -29 Mefuta ea Likokoana-hloko tsa ho Phefumoloha Sete e Kopantsoeng ea ho Fumana Asiti ea Nucleic
Thuto ea mafu a seoa
Tšoaetso ea phefumoloho ke lefu le tloaelehileng haholo ho batho, le ka hlahang bong bofe kapa bofe, lilemo le sebaka sefe kapa sefe. Ke e 'ngoe ea lisosa tse ka sehloohong tsa bokuli le lefu ho baahi lefatšeng ka bophara[1]. Likokoana-hloko tse tloaelehileng tsa phefumoloho li kenyelletsa coronavirus e ncha, vaerase ea Influenza A, vaerase ea Influenza B, vaerase ea syncytial ea phefumoloho, Adenovirus, human metapneumovirus, rhinovirus, Parainfluenza virus type I/II/III, Bocavirus, Enterovirus, Coronavirus, Mycoplasma pneumoniae, Chlamydia pneumoniae, le Streptococcus pneumoniae, jj[2,3]. Matšoao le matšoao a bakoang ke tšoaetso ea phefumoloho a tšoana haholo, empa mekhoa ea kalafo, katleho le tsela eo tšoaetso e bakoang ke likokoana-hloko tse fapaneng li fapaneng ka eona[4,5]. Hona joale, mekhoa e meholo e sebelisoang lilaboratoring ho fumana likokoana-hloko tse boletsoeng ka holimo e kenyelletsa: ho itšehla thajana ha vaerase, ho lemoha antigen le ho lemoha nucleic acid, jj. Kit ena e lemoha le ho khetholla li-nucleic acid tse itseng tsa vaerase ho batho ba nang le matšoao le matšoao a tšoaetso ea phefumoloho, ka ho lemoha ka ho thaepa livaerase tsa ntaramane le coronavirus, 'me e kopana le liphetho tse ling tsa laboratori ho fana ka thuso ho hlahlobeng tšoaetso ea tšoaetso ea phefumoloho. Liphetho tse mpe ha li qhelele ka thoko tšoaetso ea vaerase ea phefumoloho 'me ha lia lokela ho sebelisoa e le motheo feela oa tlhahlobo, kalafo, kapa liqeto tse ling tsa tsamaiso. Sephetho se setle se ke ke sa thibela tšoaetso ea baktheria kapa tšoaetso e tsoakiloeng ke likokoana-hloko tse ling tse ka ntle ho matšoao a liteko. Basebetsi ba liteko ba lokela ho ba ba fumane koetliso ea litsebi mabapi le ho holisa liphatsa tsa lefutso kapa ho lemoha baeloji ea limolek'hule, 'me ba be le mangolo a loketseng a ts'ebetso ea liteko. Laboratori e lokela ho ba le libaka tse loketseng tsa thibelo ea ts'ireletso ea biosafety le mekhoa ea ts'ireletso.
Li-parameter tsa Tekheniki
| Polokelo | -18℃ |
| Nako ea ho phela | Likhoeli tse 9 |
| Mofuta oa Sampole | Sesepa sa 'metso |
| Ct | ≤38 |
| CV | <5.0% |
| LoD | Likopi tse 200/mL |
| Ho hlaka | Liphetho tsa liteko tsa ho kopanya lisele li bontšitse hore ho ne ho se na karabelo e kopaneng pakeng tsa kit ena le Cytomegalovirus, mofuta oa 1 oa vaerase ea Herpes simplex, vaerase ea Varicella-zoster, vaerase ea Epstein-Barr, Pertussis, Corynebacterium, Escherichia coli, Haemophilus influenzae, Lactobacillus, Legionella pneumophila, Moraxella catarrhalis, mefuta e fokolitsoeng ea Mycobacterium tuberculosis, Neisseria meningitidis, Neisseria, Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes, Streptococcus salivarius, Acinetobacter baumannii, Stenotrophomonas maltophilia, Burkholderia cepacia, Corynebacterium striatum, Nocardia, Serratia marcescens, Citrobacter, Cryptococcus, Aspergillus fumigatus, Aspergillus flavus, Pneumocystis jiroveci, Candida albicans, Rothia mucilaginosus, Streptococcus oralis, Klebsiella pneumoniae, Chlamydia psittaci, Coxiella burnetii, le di-nucleic acid tsa genomic tsa batho. |
| Lisebelisoa tse Sebetsang | Mekhoa ea Li-biosystem tse Sebelisitsoeng 7500 Mekhoa ea PCR ea Nako ea 'Nete,Mekhoa ea PCR e Potlakileng ea Li-Biosystem tse 7500, QuantStudio®Mekhoa e 5 ea PCR ea Nako ea 'Nete, Mekhoa ea PCR ea Nako ea 'Nete ea SLAN-96P (Hongshi Medical Technology Co., Ltd.), Leseli la ho Tsamaisa Li-Cycler®Sistimi ea PCR ea Nako ea 'Nete ea 480, LineGene 9600 Plus Mekhoa ea ho Lemoha PCR ea Nako ea 'Nete (FQD-96A, theknoloji ea Hangzhou Bioer), Sesebelisi sa Thermal sa MA-6000 sa Nako ea 'Nete sa Bongata ba Thermal (Suzhou Molarray Co., Ltd.), Sistimi ea PCR ea BioRad CFX96 ea Nako ea 'Nete, Sistimi ea PCR ea BioRad CFX Opus 96 ea Nako ea 'Nete. |
Phallo ea Mosebetsi
Sete ea Macro & Micro-Test Viral DNA/RNA (HWTS-3017) (e ka sebelisoang le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)), le Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-8) (e ka sebelisoang le EudemonTM AIO800 (HWTS-EQ007)) ke Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.
Bophahamo ba sampole e ntšitsoeng ke 200μL mme bophahamo bo kgothaletswang ba elution ke 150μL.
