Mefuta e 17 ea HPV (16/18/6/11/44 Mofuta oa ho Ngola)
Lebitso la sehlahisoa
HWTS-CC015 Mefuta e 17 ea Papillomavirus ea Motho (16/18/6/11/44 Typing) Kit ea ho Fumana Asiti ea Nucleic (Fluorescence PCR)
Thuto ea mafu a seoa
Kankere ea molomo oa popelo ke e 'ngoe ea lihlahala tse kotsi tse atileng haholo tsamaisong ea basali ea ho ikatisa. Ho bontšitsoe hore tšoaetso ea HPV e sa khaotseng le tšoaetso e mengata ke e 'ngoe ea lisosa tse kholo tsa kankere ea molomo oa popelo. Hona joale ho ntse ho e-na le khaello ea mekhoa ea phekolo e sebetsang e amoheloang ka kakaretso bakeng sa kankere ea molomo oa popelo e bakoang ke HPV. Ka hona, ho lemoha le ho thibela tšoaetso ea molomo oa popelo e bakoang ke HPV esale pele ke linotlolo tsa ho thibela kankere ea molomo oa popelo. Ho thehoa ha liteko tse bonolo, tse tobileng le tse potlakileng tsa ho hlahloba mafu a tšoaetsanoang ke likokoana-hloko ho bohlokoa haholo bakeng sa tlhahlobo ea bongaka ea kankere ea molomo oa popelo.
Mokero
| PCR-Mix1 | FAM | 18 |
| VIC/HEX | 16 | |
| ROX | 31,33,35,39,45,51,52,56,58,59,66,68 | |
| CY5 | Taolo ea ka hare |
| PCR-Mix2 | FAM | 6 |
| VIC/HEX | 11 | |
| ROX | 44 | |
| CY5 | Taolo ea ka hare |
Li-parameter tsa Tekheniki
| Polokelo | -18℃ |
| Nako ea ho phela | Likhoeli tse 12 |
| Mofuta oa Sampole | Sampole ea moroto, sampole ea swab ea molomo oa popelo ea basali, sampole ea swab ea botšehali ea basali |
| Ct | ≤28 |
| LoD | Likopi tse 300/mL |
| Ho hlaka | Ha ho na karabelo e kopaneng le Ureaplasma urealyticum, Chlamydia trachomatis ea tšilo ea bana, Candida albicans, Neisseria gonorrhoeae, Trichomonas vaginalis, Mold, Gardnerella le mefuta e meng ea HPV e sa koaheloang ke kit. |
| Lisebelisoa tse Sebetsang | Mekhoa ea Li-biosystem tse Sebelisitsoeng 7500 Mekhoa ea PCR ea Nako ea Sebele Mekhoa ea PCR e Potlakileng ea Li-Biosystem tse 7500 tsa Nako ea 'Nete QuantStudio®Mekhoa e 5 ea PCR ea Nako ea Sebele Mekhoa ea PCR ea Nako ea Sebele ea SLAN-96P Leseli la ho Tsamaisa Li-Cycler®Mekhoa ea PCR ea Nako ea 'Nete ea 480 Litsamaiso tsa ho lemoha PCR tsa LineGene 9600 Plus tsa Nako ea 'Nete MA-6000 Nako ea Sebele ea Bongata ba Thermal Cycler Sistimi ea PCR ea BioRad CFX96 ea Nako ea 'Nete Sistimi ea PCR ea BioRad CFX Opus 96 ea Nako ea 'Nete |
Phallo ea Mosebetsi
Reagent e kgothalletswang ya ho ntsha: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (e ka sebediswang le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) ke Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Kenya 200μL ya saline e tlwaelehileng ho tjhesa pellet hape mohatong wa 2.1, ebe ho ntsha ho lokela ho etswa ho ya ka ditaelo tsa tshebediso ya reagent ena ya ho ntsha. Bophahamo bo kgothaletswang ba ho hlakisa ke 80μL.
Reagent e kgothalletswang ho ntsha: QIAamp DNA Mini Kit (51304) kapa Macro & Micro-Test Viral DNA/RNA Column (HWTS-3020-50). Kenya 200μL ya letsoai le tlwaelehileng ho kgutlisa pellet mohatong wa 2.1, ebe ho ntsha ho lokela ho etswa ho ya ka ditaelo tsa tshebediso ya reagent ena ya ho ntsha. Bongata ba sampole e ntshitsweng ba disampole kaofela ke 200μL, mme bongata bo kgothaletswang ba ho hlatswa ke 100μL.
Reagent e kgothaletswang ya ho ntsha: Reagent ya ho ntsha sampole ya Macro le Micro-Test (HWTS-3005-8I, HWTS-3005-8J, HWTS-3005-8K, HWTS-3005-8L). Kenya 200μL ya reagent ya ho ntsha sampole ho kgutlisa pellet mohatong wa 2.1, ebe ho ntshwa ha yona ho lokela ho etswa ho ya ka ditaelo tsa tshebediso ya reagent ena ya ho ntsha.
