Mefuta e 17 ea HPV (16/18/6/11/44 Typing)
Lebitso la sehlahisoa
HWTS-CC015 17 Mefuta ea Human Papillomavirus (16/18/6/11/44 Typing) Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Kankere ea molomo oa popelo ke e 'ngoe ea lihlahala tse kotsi tse atileng haholo lesakeng la tsoalo la basali. Ho bonts'itsoe hore ts'oaetso ea HPV e phehellang le mafu a mangata ke e 'ngoe ea lisosa tse kholo tsa mofetše oa molomo oa popelo. Hajoale ho ntse ho na le khaello ea liphekolo tse amoheloang ka kakaretso tse sebetsang bakeng sa mofetše oa popelo o bakoang ke HPV. Ka hona, ho lemoha kapele le thibelo ea tšoaetso ea molomo oa popelo e bakoang ke HPV ke linotlolo tsa thibelo ea kankere ea molomo oa popelo. Ho thehoa ha liteko tse bonolo, tse tobileng le tse potlakileng tsa ho hlahloba likokoana-hloko ho bohlokoa haholo bakeng sa tlhahlobo ea bongaka ea kankere ea molomo oa popelo.
Channel
| PCR-Mix1 | FAM | 18 |
| VIC/HEX | 16 | |
| ROX | 31,33,35,39,45,51,52,56,58,59,66,68 | |
| CY5 | Taolo ya ka hare |
| PCR-Mix2 | FAM | 6 |
| VIC/HEX | 11 | |
| ROX | 44 | |
| CY5 | Taolo ya ka hare |
Tekheniki Parameters
| Polokelo | -18℃ |
| Shelf-bophelo | Likhoeli tse 12 |
| Mofuta oa Mohlala | Mohlala oa moroto, sampole ea sebono sa molomo oa popelo ea mosali, sesupa sa setho sa botšehali sa mosali |
| Ct | ≤28 |
| LoD | 300Likopi / mL |
| Tse khethehileng | Ha ho na cross-reactivity le Ureaplasma urealyticum, Chlamydia trachomatis ea pampitšana ea ho ikatisa, Candida albicans, Neisseria gonorrhoeae, Trichomonas vaginalis, Mold, Gardnerella le mefuta e meng ea HPV e sa koaheloang ke kit. |
| Lisebelisoa tse sebetsang | Applied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®Litsamaiso tse 5 tsa nako ea 'nete tsa PCR Litsamaiso tsa PCR tsa nako ea sebele tsa SLAN-96P LightCycler®Litsamaiso tsa PCR tsa Nako ea 480 LineGene 9600 Plus Nako ea 'Nete ea PCR ea ho Khetholla Sistimi MA-6000 Real-Nako Quantitative Thermal Cycler Sistimi ea PCR ea Nako ea 'Nete ea BioRad CFX96 BioRad CFX Opus 96 Real-Nako PCR System |
Phallo ea Mosebetsi
Sehlahisoa se khothalelitsoeng: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (e ka sebelisoang ka Macro & Micro-Test Automatic Nucleic Acid006W Extra HWTS-3006B)) ka Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Eketsa 200μL ea saline e tloaelehileng ho khutlisetsa pellet mohatong oa 2.1, ebe ho ntšoa ho lokela ho etsoa ho latela litaelo tsa tšebeliso ea reagent ena ea ho ntša. Molumo o khothaletsoang oa elution ke 80μL.
Sehlahisoa se khothalelitsoeng: QIAamp DNA Mini Kit (51304) kapa Macro & Micro-Test Viral DNA/RNA Column (HWTS-3020-50). Eketsa 200μL ea saline e tloaelehileng ho emisa pellet mohatong oa 2.1, 'me joale ho ntšoa ho lokela ho etsoa ho latela litaelo tsa tšebeliso ea reagent ena. Bolumo ea sampole e ntšitsoeng ea lisampole kaofela ke 200μL, 'me bophahamo ba molumo o khothaletsoang ke 100μL.
Sehlahisoa sa ho ntša se khothalelitsoeng: Macro & Micro-Test Sample Release Reagent (HWTS-3005-8I, HWTS-3005-8J, HWTS-3005-8K, HWTS-3005-8L). Eketsa 200μL ea reagent ea tokollo ea sampole ho emisa pellet mohatong oa 2.1, ebe ho ntšoa ho lokela ho etsoa ho latela litaelo tsa tšebeliso ea reagent ena.
