Mefuta e 17 ea HPV (16/18/6/11/44 Typing)

Tlhaloso e Khutšoanyane:

Setsi sena se loketse ho lemoha boleng ba mefuta e 17 ea papillomavirus ea motho (HPV) (HPV 6, 11, 16,18,31, 33,35, 39, 44,45, 51, 52.56,58, 59,66), 68) likaroloana tse itseng tsa "nucleic acid" sampoleng ea moroto, sampole ea sekoti sa molomo oa popelo ea mosali le sampole ea setho sa botšehali sa mosali, le HPV 16/18/6/11/44 ho thusa ho hlahloba le ho alafa tšoaetso ea HPV.


Lintlha tsa Sehlahisoa

Li-tag tsa Sehlahisoa

Lebitso la sehlahisoa

HWTS-CC015 17 Mefuta ea Human Papillomavirus (16/18/6/11/44 Typing) Nucleic Acid Detection Kit (Fluorescence PCR)

Epidemiology

Kankere ea molomo oa popelo ke e 'ngoe ea lihlahala tse kotsi tse atileng haholo lesakeng la tsoalo la basali.Ho bonts'itsoe hore ts'oaetso ea HPV e phehellang le mafu a mangata ke e 'ngoe ea lisosa tse kholo tsa mofetše oa molomo oa popelo.Hajoale ho ntse ho na le khaello ea liphekolo tse amoheloang ka kakaretso tse sebetsang bakeng sa mofetše oa molomo oa popelo o bakoang ke HPV.Ka hona, ho lemoha kapele le thibelo ea tšoaetso ea molomo oa popelo e bakoang ke HPV ke linotlolo tsa thibelo ea kankere ea molomo oa popelo.Ho thehoa ha liteko tse bonolo, tse tobileng le tse potlakileng tsa ho hlahloba likokoana-hloko ho bohlokoa haholo bakeng sa tlhahlobo ea bongaka ea kankere ea molomo oa popelo.

Channel

PCR-Mix1 FAM 18
VIC/HEX

16

ROX

31,33,35,39,45,51,52,56,58,59,66,68

CY5 Taolo ya ka hare
PCR-Mix2 FAM 6
VIC/HEX

11

ROX

44

CY5 Taolo ya ka hare

Tekheniki Parameters

Polokelo

-18℃

Shelf-bophelo Likhoeli tse 12
Mofuta oa Mohlala Mohlala oa moroto, sampole ea sekoti sa molomo oa popelo ea mosali, sesupa sa botšehali sa mosali
Ct ≤28
LoD 300Likopi / mL
Tse khethehileng Ha ho na cross-reactivity le Ureaplasma urealyticum, Chlamydia trachomatis ea pampitšana ea pelehi, Candida albicans, Neisseria gonorrhoeae, Trichomonas vaginalis, Mold, Gardnerella le mefuta e meng ea HPV e sa koaheloang ke kit.
Lisebelisoa tse sebetsang Applied Biosystems 7500 Real-Time PCR Systems
Applied Biosystems 7500 Fast Real-Time PCR Systems
QuantStudio®Litsamaiso tse 5 tsa nako ea 'nete tsa PCR
Litsamaiso tsa PCR tsa nako ea sebele tsa SLAN-96P
LightCycler®Litsamaiso tsa PCR tsa Nako ea 480
LineGene 9600 Plus Nako ea 'Nete ea PCR ea ho Khetholla Sistimi
MA-6000 Real-Nako Quantitative Thermal Cycler
Sistimi ea PCR ea Nako ea 'Nete ea BioRad CFX96
BioRad CFX Opus 96 Real-Nako PCR System

Phallo ea Mosebetsi

Sehlahisoa se khothalelitsoeng: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (e ka sebelisoang le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) ka Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Kenya 200μL ea letsoai le tloaelehileng ho emisa pellet mohatong oa 2.1, ebe ho ntšoa ho lokela ho etsoa ho latela ho litaelo tsa tšebeliso ea reagent ena ea ho ntša.Molumo o khothaletsoang oa elution ke 80μL.

Sehlahisoa se khothalelitsoeng: QIAamp DNA Mini Kit (51304) kapa Macro & Micro-Test Viral DNA/RNA Column (HWTS-3020-50).Eketsa 200μL ea letsoai le tloaelehileng ho emisa pellet mohatong oa 2.1, 'me joale ho ntšoa ho lokela ho etsoa ho latela litaelo tsa tšebeliso ea reagent ena.Bolumo ea sampole e nkiloeng ea lisampole kaofela ke 200μL, 'me bophahamo ba molumo o khothaletsoang ke 100μL.

Sehlahisoa sa ho ntša se khothalelitsoeng: Macro & Micro-Test Sample Release Reagent (HWTS-3005-8I, HWTS-3005-8J, HWTS-3005-8K, HWTS-3005-8L).Eketsa 200μL ea reagent ea ho lokolla sampole ho emisa pellet mohatong oa 2.1, ebe ho ntšoa ho lokela ho etsoa ho latela litaelo tsa ts'ebeliso ea reagent ena.


  • E fetileng:
  • E 'ngoe:

  • Ngola molaetsa wa hao mona mme o re romele wona