Mefuta e 14 ea Likokoana-hloko tse Phefumolohang tse Kopantsoeng
Lebitso la sehlahisoa
HWTS-RT159B Mefuta e 14 ea Likokoana-hloko Tse Phefumolohang tse Kopantsoeng Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Tšoaetso ea phefumoloho ke lefu le tloaelehileng ka ho fetisisa ho batho, le ka hlahang ho bong leha e le bofe, lilemo le sebaka. Ke e 'ngoe ea lisosa tse ka sehloohong tsa ho kula le ho shoa ha batho lefatšeng ka bophara[1]. Likokoana-hloko tse tloaelehileng tsa phefumoloho li kenyelletsa coronavirus e ncha, kokoana-hloko ea Influenza A, kokoana-hloko ea Influenza B, Respiratory syncytial virus, Adenovirus, human metapneumovirus, rhinovirus, Parainfluenza virus mofuta oa I/II/III/IV, Bocavirus, Enterovirus, Coronavirus, Mycoplasma pneumoniae, Chlamydia pneumoniae pneumoniae, St.[2,3].
Channel
| Hantle Boemo | Lebitso la Tharollo ea Maikutlo | Likokoana-hloko tse lokelang ho fumanoa |
| 1 | Master Mix A | SARS-CoV-2, IFV A, IFV B |
| 2 | Master Mix B | Adv, hMPV, MP, Cpn |
| 3 | Master Mix C | PIVI/II/III/IV, Rhv, RSV, HBoV |
| 4 | Master Mix D | CoV, EV, SP, taolo ea ka hare |
| 5 | Master Mix A | SARS-CoV-2, IFV A, IFV B |
| 6 | Master Mix B | Adv, hMPV, MP, Cpn |
| 7 | Master Mix C | PIVI/II/III/IV, Rhv, RSV, HBoV |
| 8 | Master Mix D | CoV, EV, SP, taolo ea ka hare |
Tekheniki Parameters
| Polokelo | ≤-18℃ |
| Shelf-bophelo | Likhoeli tse 9 |
| Mofuta oa Mohlala | Oropharyngeal swab, Nasopharyngeal swab |
| Ct | ≤38 |
| CV | <5.0% |
| LoD | 200Likopi/mL |
| Tse khethehileng | Liphetho tsa tlhahlobo ea cross-reactivity li bonts'itse hore ho ne ho se na karabelo lipakeng tsa kit ena le Cytomegalovirus, Herpes simplex virus type 1, Varicella-zoster virus, Epstein-Barr virus, Bordetella pertussis, Corynebacterium, Escherichia coli, Haemophilus influenzae, Lactobacillus, Legionella-zoster virus, Camophilus influenzae Lefuba la Mycobacterium, Neisseria meningitidis, Neisseria, Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes, Streptococcus salivarius, Acinetobacter baumannii, Stekhomalcoccus epidermidis, Coccus pyogenes, Streptococcus salivarius, Acinetobacter baumannii, Cocomal bacterium Bursemonace. striatum, Nocardia, Serratia marcescens, Citrobacter, Cryptococcus, Aspergillus fumigatus, Aspergillus flavus, Pneumocystis jiroveci, Candida albicans, Rothia mucilaginosus, Streptococcus oralis, Klebsiella pneumoniae, Chlaciimic nucleic acid e chesa batho. |
| Lisebelisoa tse sebetsang | SLAN-96P ea Nako ea Sebele PCR Systems (Hongshi Medical Technology Co., Ltd.)Applied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®Litsamaiso tse 5 tsa nako ea 'nete tsa PCR LineGene 9600 Plus Nako ea 'Nete ea PCR Detection Systems (FQD-96A, theknoloji ea Hangzhou Bioer) MA-6000 Nako ea 'Nete ea Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) BioRad CFX96 Real-Nako PCR System, BioRad CFX Opus 96 Real-Nako PCR System |
Phallo ea Mosebetsi
Sehlahisoa se khothalelitsoeng sa ho ntšoa: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (e ka sebelisoang le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ010)) by Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. L20 sampole e nkiloeng. Mehato e latelang e lokela ho etsoa ho latela litaelo tsa tšebeliso ea reagent ena. Molumo o khothaletsoang oa elution ke80µL.
