Xinjiang Hemorrhagic Fever virus
Lebitso la sehlahisoa
HWTS-FE007B/C Xinjiang Hemorrhagic Fever Virus Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Xinjiang hemorrhagic fever virus e qalile ho aroloa maling a bakuli ba tšoeroeng ke feberu ea hemorrhagic sebakeng sa Tarim Basin, Xinjiang, China le liboseleise tse thata tse hapiloeng sebakeng seo, 'me tsa reha lebitso la tsona. Lipontšo tsa kliniki li kenyelletsa feberu, hlooho, ho tsoa mali, ho tšoha ha hypotensive, joalo-joalo The liphetoho tsa motheo tsa pathological tsa lefu lena ke systemic capillary dilatation, congestion, eketseha permeability le fragility, ho fella ka mefuta e sa tšoaneng ea tšubuhlellano le tšollo ea mali ka letlalo le mucous lera hammoho le dinama tse nyenyane tse ea litho tse fapa-fapaneng ho pholletsa le 'mele, ka degeneration le tšoelesa joalo ka necrosis, tšoelesa ea adrenal, tšoelesa ea liphio, joalo-joalo. edema e kang jelly ka har'a retroperitoneum.
Channel
| FAM | Xinjiang Hemorrhagic Fever virus |
| ROX | Taolo ea ka hare |
Tekheniki Parameters
| Polokelo | ≤-18℃ |
| Shelf-bophelo | Likhoeli tse 9 |
| Mofuta oa Mohlala | serum e ncha |
| Tt | ≤38 |
| CV | ＜5.0% |
| LoD | 1000Likopi/mL |
| Tse khethehileng | Ha ho cross-reactivity le lisampole tse ling tsa phefumoloho tse kang Influenza A, Influenza B, Legionella pneumophila, Rickettsia Q fever, Chlamydia pneumoniae, Adenovirus, Respiratory Syncytial Virus, Parainfluenza 1, 2, 3, kokoana-hloko ea Coxsackie, kokoana-hloko ea Echo, Metapnevirus A1/B2 syncytial virus A/B, Coronavirus 229E/NL63/HKU1/OC43, Rhinovirus A/B/C, Boca virus 1/2/3/4, Chlamydia trachomatis, adenovirus, joalo-joalo le DNA ea genomic ea motho. |
| Lisebelisoa tse sebetsang | Applied Biosystems 7500 Real-Nako PCR Systems, Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®Litsamaiso tse 5 tsa nako ea 'nete tsa PCR Litsamaiso tsa PCR tsa nako ea sebele tsa SLAN-96P LightCycler®Sistimi ea 480 ea Nako ea 'Nete ea PCR LineGene 9600 Plus Nako ea 'Nete ea PCR ea ho Khetholla Sistimi MA-6000 Real-Nako Quantitative Thermal Cycler Sistimi ea PCR ea Nako ea 'Nete ea BioRad CFX96 BioRad CFX Opus 96 Real-Nako PCR System |
Phallo ea Mosebetsi
Recommended reagent: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (e ka sebelisoang le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) by Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Bolumo ea sampole e ntšitsoeng ke 200µL, 'me bophahamo ba modumo e khothaletsoang ke 80µL.
Sehlahisoa se khothalelitsoeng sa ho ntšoa: QIAamp Viral RNA Mini Kit (52904) ka QIAGEN le Nucleic Acid Extraction kapa Purification Reagent (YDP315-R). Ho ntšoa ho lokela ho etsoa ho latela litaelo tsa tšebeliso. Bolumo ea sampole e ntšitsoeng ke 140µL, 'me bophahamo ba modumo e khothaletsoang ke 60µL.
