Orientia tsutsugamushi Nucleic Acid
Lebitso la sehlahisoa
HWTS-OT002-Orientia tsutsugamushi Nucleic Acid Detection Kit(Fluorescence PCR)
Epidemiology
Scrub typhus ke lefu le matla la febrile le bakoang ke tšoaetso ea Orientia tsutsugamushi (Ot). Orientia scrub typhus ke gram-negative obligate intracellular parasitic microorganism. Orientia scrub typhus ke ea mofuta oa Orientia ka tatellano ea Rickettsiales, lelapa la Rickettsiaceae, le mofuta oa Orientia. Scrub typhus e fetisoa haholo-holo ka ho longoa ke li-chigger larvae tse jereng likokoana-hloko. E tšoauoa ka tleliniking ka tšohanyetso feberu e phahameng, eschar, lymphadenopathy, hepatosplenomegaly, le peripheral mali leukopenia, joalo-joalo Maemong a matla, ho ka etsa hore meningitis, sebete le liphio ho hloleha, systemic tse ngata tsa setho ho hloleha, esita le lefu.
Tekheniki Parameters
| Polokelo | ≤-18℃ |
| Shelf-bophelo | Likhoeli tse 12 |
| Mofuta oa Mohlala | lisampole tsa serum |
| CV | ≤5.0% |
| LoD | 500 Likopi / μL |
| Lisebelisoa tse sebetsang | E sebetsa ho mochini oa ho lemoha oa mofuta oa I: Applied Biosystems 7500 Real-Nako PCR Systems, QuantStudio®Litsamaiso tse 5 tsa nako ea 'nete tsa PCR, SLAN-96P ea Nako ea Sebele PCR Systems (Hongshi Medical Technology Co., Ltd.), LineGene 9600 Plus Nako ea 'Nete ea PCR Detection Systems (FQD-96A, theknoloji ea Hangzhou Bioer), MA-6000 Real-Nako Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.), Sistimi ea PCR ea Nako ea 'Nete ea BioRad CFX96, BioRad CFX Opus 96 Real-Nako PCR System. E sebetsa ho mofuta oa II reagent ea ho lemoha: EudemonTMAIO800 (HWTS-EQ007) ka Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. |
Phallo ea Mosebetsi
Macro & Micro-Test General DNA/RNA Kit (HWTS-3019) (e ka sebelisoang le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) by Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ho ntšoa ho lokela ho etsoa ho latela taelo ea ts'ebeliso ea reagent. Bolumo ea sampole e ntšitsoeng ke 200μL, 'me molumo o khothaletsoang oa elution ke 100μL.
