—— Phuputso e Kopanetsoeng ke Zhejiang CDC, Macro & Micro-Test, le China CDC E phatlalalitsoe ho Frontiers in Cellular and Infection Microbiology
Kakaretso ea Thuto
Ka Mots'eanong 2026, Frontiers in Cellular and Infection Microbiology (JCR Q1, IF ≈ 4.6) e phatlalalitse pampiri e etelletsoeng pele ke Setsi sa Taolo le Thibelo ea Mafu sa Zhejiang Provincial Center for Disease Control and Prevention (Zhejiang CDC), hammoho le sehlopha sa bioinformatics se tsoang Beijing Macro & Micro-Test Bio-Tech Co., Ltd. le Setsi sa Naha sa Taolo le Thibelo ea Mafu a Tšoaetsanoang (China CDC) e le bangoli-'moho. Phuputso ena e na le sehlooho se reng:
"Tlhahlobo le tlhahlobo ea phylogenetic ea mefuta e supileng ea Brucella abortus e Zhejiang, Chaena."
Phuputso ena e emela tlhahlobo ea pele ea ho latela mokhoa oa ho latela Brucella abortus (B. abortus) e thehiloeng ho phylogenetic e hlophisitsoeng, e thehiloeng ho genome eohle Profinseng ea Zhejiang, Chaena. Sehlopha se hlahlobile mefuta e supileng e arohaneng e bokelletsoeng ho tloha ka 2015 ho isa ho 2025 (mefuta e mene e tsoang ho batho le mefuta e meraro e tsoang ho likhomo e tsoang Jinhua, Quzhou le Ningbo). Liphuputso li fana ka bopaki ba genomic bakeng sa tšimoloho le litsela tsa phetisetso ea "mofuta ona o ka sehloohong o ka leboea" sebakeng sa seoa se sa tloaelehang sa boroa sa Chaena e ka bochabela.
Semelo le Bohlokoa
Brucellosis ke lefu la zoonotic le bakoang ke baktheria ea mofuta oa Brucella. Brucella abortus e tšoaetsa likhomo haholo-holo empa e ka boela ea baka mafu ho batho. Chaena, brucellosis e bontša phapang e totobetseng ea libaka: palo e phahameng ka ho fetisisa ea batho e etsahala liprofinseng tse ka leboea (mohlala, Inner Mongolia, Shanxi, Heilongjiang). Ka lehlakoreng le leng, liprofinse tse ka boroa, ho kenyeletsoa le Zhejiang, li 'nile tsa busoa ke Brucella melitensis historing, ka linyeoe tse fokolang haholo tse tlalehiloeng tsa B. abortus. Phapang ena ea libaka e etsa hore tlhaloso ea liphatsa tsa lefutso le ho latela mohloli oa B. abortus Zhejiang e be ntho ea bohlokoa bophelong ba sechaba.
Mekhoa le Liphihlelo tsa Bohlokoa
Sehlopha sa bafuputsi se amohetse leano la mahlakore a mangata le kopanyang baeloji ea limolek'hule le bioinformatics:
1.Tlhahlobo ea kokoana-hloko le ho thaepa ha motheo
BCSP-31 gene PCR le AMOS-PCR li tiisitse hore li-isolates tsohle tse supileng e ne e le B. abortus.
Mofuta wa ho ngola tatellano ya dijini tse ngata (MLST) o itshetlehileng hodima dijini tse robong tsa tlhokomelo ya ntlo o senotse hore di-isolates tsohle di ne di le tsa mofuta wa tatellano ya ST2, e leng se bontshang ho tshwana ho hoholo ha dijini hara mefuta ya B. abortus e potolohang Zhejiang.
2.Tlhaloso ea liphatsa tsa lefutso tse felletseng
Ho latellana ha liphatsa tsa lefutso tse felletseng ho entsoe sethaleng sa Illumina NovaSeq. Tlhahlobo e tloaelehileng ea boitsebiso ba nucleotide (ANI) e bontšitse hore li-isolates tsa Zhejiang li arolelane ho tšoana ho fihla ho 99.99% le mofuta oa referense oa B. abortus 544.
Tlhahlobo ea Pan-genome e senotse palo e bolokiloeng haholo ea batho: liphatsa tsa lefutso tsa mantlha tse 3,084 li ile tsa fumanoa, hammoho le liphatsa tsa lefutso tse 10 feela tsa khetla, 'me ha ho liphatsa tsa lefutso tsa motheo o bonolo kapa oa leru tse ileng tsa fumanoa.
3.Liprofaele tsa liphatsa tsa lefutso tse hanyetsanang le kokoana-hloko le tse bolaeang likokoana-hloko
Ho boletsoe esale pele lintlha tse 68 tse amanang le kokoana-hloko, tse akaretsang litsela tsa khale tse kang LPS biosynthesis, sistimi ea ho ntša metsi ea T4SS, le sistimi ea taolo ea likarolo tse peli ea BvrR-BvrS. Haholo-holo, li-isolates tsohle li ne li haelloa ke liphatsa tsa lefutso tsa adhesin bmaA le btaF. Tlhahlobo ea liphatsa tsa lefutso tsa khanyetso e fumane feela liphatsa tsa lefutso tsa mprF polokelongtshedimosetsong ea CARD, 'me ha ho na lintho tse ling tse khethollang khanyetso tse fumanoeng.
4. Ho tsosolosoa ha liphatsa tsa lefutso le ho latela phetisetso
Tlhahlobo ea Core-genome single-nucleotide polymorphism (cgSNP) e behile li-isolate tsa Zhejiang sebakeng se itseng sefateng sa lefats'e sa phylogenetic. Liphetho li bontšitse hore mefuta ea Zhejiang e theha sehlopha sa monophyletic hammoho le mefuta e tsoang Russia, Mongolia, le liprofinse tse 'maloa tsa leboea la Chaena (Ningxia, Heilongjiang, Inner Mongolia, Hebei, Gansu, Beijing). Sehlopha sena se boetse se aroha ka lihlopha tse tharo tse fapaneng (Clade 1-3), se bontšang liketsahalo tse ngata tse ikemetseng tsa kenyelletso.
Liqeto le Litlamorao
Phuputso ena e fana ka sete ea pele ea data ea genomic e nepahetseng haholo ea B. abortus Profinseng ea Zhejiang 'me e fana ka liqeto tse' maloa tsa bohlokoa:
- Clear semelo sa lefutso– Mefuta ea B. abortus e potolohang Zhejiang ke ea ST2, e bolokiloe haholo ka liphatsa tsa lefutso, 'me e emela leloko le tloaelehileng la brucellosis ea likhomo.
2. Evidence ea phetiso ea libaka tse fapaneng– Tlhahlobo ea phylogenetic ha e tšehetse boteng ba leloko le ikemetseng la tlhaho Zhejiang. Ho e-na le hoo, lintlha li bontša ka matla hore mefuta ena e simolohile karolong e ka leboea ea Chaena 'me e ka arolelana semelo se tšoanang sa ho iphetola ha lintho le mefuta e tsoang Russia le Mongolia. Boteng ba lihlopha tse tharo tse nyenyane bo bolela liketsahalo tse ngata tse arohaneng tsa kenyelletso.
3. Litlamorao tsa bophelo bo botle ba sechaba– Diphuputso di totobatsa bohlokwa ba tlhokomelo ya genomic bakeng sa brucellosis esita le dibakeng tse sa atileng haholo tse kang Zhejiang. Le hoja palo ya dinyewe tsa hajwale e le tlase, disebediswa tse nang le qeto e phahameng jwalo ka cgSNP di ka latela ka katleho mohlodi wa mafu a tswelang kantle ho naha mme tsa fana ka bopaki ba saense ho sitisa diketane tsa phetisetso tse amanang le dipalangwang tsa mehlape hara diprovense.
Mosebetsi ona ha o tlatse feela sekheo sa lipatlisiso Profenseng ea Zhejiang empa hape o fana ka lintlha tse ncha tsa motheo bakeng sa tlhokomelo ea likokoana-hloko le tlhahlobo ea kotsi ea brucellosis sebakeng sa Noka ea Yangtze Delta.
Tlhahisoleseding ea Pampiri:
Yang, Y., Shi, X., Chen, J., Wang, L., Wu, Z., Yao, W., … & Wu, B. (2026). Tlhahlobo ea ho khetholla le ho hlahloba liphatsa tsa lefutso tsa mefuta e supileng ea Brucella abortus e Zhejiang, Chaena. Frontiers in Cellular and Infection Microbiology, 16, 1758965.
Nako ea poso: Phuptjane-10-2026