Mycobacterium Tuberculosis INH Phetoho
Lebitso la sehlahisoa
HWTS-RT137 Mycobacterium Tuberculosis INH Mutation Detection Kit (Melting Curve)
Epidemiology
Mycobacterium tuberculosis, haufinyane joalo ka Tubercle bacillus (TB), ke baktheria ea pathogenic e bakang lefuba. Hona joale, meriana ea pele e sebelisoang ka tloaelo e khahlanong le lefuba e kenyelletsa INH, rifampicin le hexambutol, joalo-joalo Lithethefatsi tse khahlanong le lefuba la bobeli li kenyelletsa fluoroquinolones, amikacin le kanamycin, joalo-joalo Lithethefatsi tse ncha tse tsoetseng pele ke linezolid, bedaquiline le delamani, joalo-joalo, ka lebaka la tšebeliso e fosahetseng ea anti-tuberculosis lerako la mycobacteriosis, leha ho le joalo, ka lebaka la tšebeliso e fosahetseng ea anti-tuberculosis lerako la mycobacteriosis, mycobacterium tuberculosis e hlahisa khanyetso ea lithethefatsi khahlanong le litlhare tse thibelang lefuba, e leng se tlisang mathata a tebileng thibelong le kalafong ea lefuba.
Channel
| FAM | MP nucleic acid |
| ROX | Taolo ea ka hare |
Tekheniki Parameters
| Polokelo | ≤-18℃ |
| Shelf-bophelo | Likhoeli tse 12 |
| Mofuta oa Mohlala | sekhohlela |
| CV | ≤5% |
| LoD | Moeli oa ho lemoha bakeng sa libaktheria tsa mofuta oa naha oa INH ke 2x103 bacteria/mL, 'me moeli oa ho lemoha bakeng sa baktheria e fetohang ke 2x103 bacteria/mL. |
| Tse khethehileng | a. Ha ho na karabelo e fapaneng har'a genome ea motho, likokoana-hloko tse ling tsa mycobacteria le pneumonia tse fumanoeng ke setsi sena.b. Libaka tsa phetoho ea liphatsa tsa lefutso tse hanyetsanang le lithethefatsi tse hlaha ho Mycobacterium tuberculosis, joalo ka sebaka se khethollang khanyetso ea mofuta oa rifampicin rpoB, li ile tsa fumanoa, 'me liphetho tsa liteko li bontšitse hore ha li hanyetsane le INH, tse bontšang ho se sebetse hape. |
| Lisebelisoa tse sebetsang | Litsamaiso tsa PCR tsa nako ea sebele tsa SLAN-96PBioRad CFX96 Real-Nako PCR SystemsLightCycler480®Sistimi ea nako ea 'nete ea PCR |
Phallo ea Mosebetsi
Haeba u sebelisa Macro & Micro-Test General DNA/RNA Kit (HWTS-3019) (e ka sebelisoang le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) ka Jiangsu Macro & Micro-Test Med-Tech Coction, Ltd. sampuli ea sekhohlela e lekoa ka tatellano, 'me u kenyelle 10μL ea taolo ea ka hare ka thoko taolong e mpe, sampole ea sekhohlela e sebelitsoeng ho hlahlojoa,' me mehato e latelang e lokela ho etsoa ka thata ho latela litaelo tsa ho ntšoa. Bolumo ea sampole e ntšitsoeng ke 200μL, 'me molumo o khothaletsoang oa elution ke 100μL.
