Phetoho ea Mycobacterium Tuberculosis INH
Lebitso la sehlahisoa
Kiti ea ho lemoha phetoho ea liphatsa tsa lefutso ea HWTS-RT137 Mycobacterium Tuberculosis INH (Melting Curve)
Thuto ea mafu a seoa
Mycobacterium tuberculosis, e leng Tubercle bacillus (TB) ka bokhutšoanyane, ke baktheria e bakang lefuba. Hona joale, meriana ea pele e sebelisoang haholo ea ho loantša lefuba e kenyelletsa INH, rifampicin le hexambutol, jj. Meriana ea bobeli ea ho loantša lefuba e kenyelletsa fluoroquinolones, amikacin le kanamycin, jj. Meriana e mecha e ntlafalitsoeng ke linezolid, bedaquiline le delamani, jj. Leha ho le joalo, ka lebaka la tšebeliso e fosahetseng ea meriana ea ho loantša lefuba le litšobotsi tsa sebopeho sa lebota la sele la mycobacterium tuberculosis, mycobacterium tuberculosis e hlahisa ho hanyetsa meriana ea ho loantša lefuba, e leng se tlisang liphephetso tse kholo thibelong le kalafong ea lefuba.
Mokero
| FAM | Asiti ea nucleic ea MP |
| ROX | Taolo ea Ka Hare |
Li-parameter tsa Tekheniki
| Polokelo | ≤-18℃ |
| Nako ea ho phela | Likhoeli tse 12 |
| Mofuta oa Sampole | sekhohlela |
| CV | ≤5% |
| LoD | Moeli oa ho lemoha libaktheria tsa mofuta oa INH oa hlaha ke baktheria ea 2x103/mL, 'me moeli oa ho lemoha libaktheria tse fetotsoeng ke baktheria ea 2x103/mL. |
| Ho hlaka | a. Ha ho na karabelo e kopaneng hara genome ea motho, likokoana-hloko tse ling tse seng tsa TB le pneumonia tse fumanoang ke kit ena.b. Libaka tsa phetoho ea liphatsa tse ling tsa lefutso tse hanyetsanang le meriana ho Mycobacterium tuberculosis ea mofuta oa tlhaho, tse kang sebaka se khethollang khanyetso ea rifampicin rpoB gene, li ile tsa fumanoa, 'me liphetho tsa liteko li ile tsa bontša ho se hanyetse INH, tse bontšang hore ha ho na karabelo e kopaneng. |
| Lisebelisoa tse Sebetsang | Mekhoa ea PCR ea Nako ea Sebele ea SLAN-96PMekhoa ea PCR ea BioRad CFX96 ea Nako ea 'NeteLightCycler480®Sistimi ea PCR ea Nako ea Sebele |
Phallo ea Mosebetsi
Haeba o sebedisa Macro & Micro-Test General DNA/RNA Kit (HWTS-3019) (e ka sebediswang le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) ya Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. bakeng sa ho ntsha, eketsa 200μL ya taolo e mpe le sampole ya sekhohlela e sebeditsweng e tla lekwa ka tatellano, mme o kenye 10μL ya taolo ya kahare ka thoko ho sampole ya sekhohlela e mpe, e sebeditsweng e tla lekwa, mme mehato e latelang e lokela ho etswa ka thata ho ya ka ditaelo tsa ho ntsha. Bophahamo ba sampole e ntshitsweng ke 200μL, mme bophahamo bo kgothaletswang ba ho hlatswa ke 100μL.
