MTHFR Gene Polymorphic Nucleic Acid

Tlhaloso e Khutšoanyane:

Sesebelisoa sena se sebelisetsoa ho bona libaka tse 2 tsa phetoho ea mofuta oa MTHFR.Thepa e sebelisa mali a motho kaofela e le sampole ea teko ho fana ka tlhahlobo ea boleng ba boemo ba phetoho.E ka thusa baoki ho etsa merero ea kalafo e loketseng litšobotsi tse fapaneng tsa motho ka mong ho tloha boemong ba limolek'hule, e le ho netefatsa bophelo bo botle ba bakuli ho isa tekanyong e kholo.


Lintlha tsa Sehlahisoa

Li-tag tsa Sehlahisoa

Lebitso la sehlahisoa

HWTS-GE004-MTHFR Gene Polymorphic Nucleic Acid Detection Kit (ARMS-PCR)

Epidemiology

Folic acid ke vithamine e qhibilihang ka metsing eo e leng motsoako oa bohlokoa litseleng tsa 'mele oa metabolism.Lilemong tsa morao tjena, palo e kholo ea liphuputso e tiisitse hore, phetoho ea folate metabolizing enzyme gene MTHFR e tla lebisa khaellong ea folic acid 'meleng,' me tšenyo e tloaelehileng ea khaello ea folic acid ho batho ba baholo e ka baka megaloblastic anemia, vascular. tšenyo ea endothelial, joalo-joalo Ho haella ha folic acid ho basali ba bakhachane ha ba khone ho khotsofatsa litlhoko tsa bona le lesea le ka pōpelong, e leng se ka bakang bokooa ba methapo ea kutlo, anencephaly, pelehi, le ho senyeheloa ke mpa.Maemo a serum folate a angoa ke 5,10-methylenetetrahydrofolate reductase (MTHFR) polymorphisms.Liphetoho tsa 677C> T le 1298A> C ho liphatsa tsa lefutso tsa MTHFR li etsa hore ho fetoloa ha alanine ho valine le glutamic acid, ka ho latellana, ho fella ka ho fokotsa mosebetsi oa MTHFR 'me ka lebaka leo ho fokotsehe ho sebelisoa ha folic acid.

Channel

FAM MTHFR C677T
ROX MTHFR A1298C
VIC(HEX) Taolo ya ka hare

Tekheniki Parameters

Polokelo

≤-18℃

Shelf-bophelo

Likhoeli tse 12

Mofuta oa Mohlala

Mali a sa tsoa bokelloa a EDTA a anticoagulated

CV

≤5.0%

Ct

≤38

LoD

1.0ng/μL

Lisebelisoa tse Sebetsang:

Applied Biosystems 7500 Real-Time PCR Systems

Applied Biosystems 7500 Fast Real-Time PCR Systems

SLAN ®-96P ea Nako ea 'Nete PCR Systems

QuantStudio™ 5 ea Nako ea 'Nete ea PCR Systems

Sesebelisoa sa PCR sa LightCycler®480 Real-Time

LineGene 9600 Plus Nako ea 'Nete ea PCR ea ho Fumana Sistimi

MA-6000 Real-Nako Quantitative Thermal Cycler

Sistimi ea PCR ea Nako ea 'Nete ea BioRad CFX96

BioRad CFX Opus 96 Real-Nako PCR System

Phallo ea Mosebetsi

Khetho ea 1

Li-reagents tse khothalelitsoeng: Macro & Micro-Test Genomic DNA Kit (HWTS-3014-32, HWTS-3014-48, HWTS-3014-96) le Macro & Micro-Test Automatic Nucleic Acid Extractor(HWTS-3006C, HWBTS-300) .

Khetho ea 2

Li-reagents tse khothalelitsoeng: Blood Genomic DNA Extraction Kit(YDP348, JCXB20210062) by Tiangen Biotech(Beijing) Co.,Ltd.Mali a Genome Extraction Kit(A1120) ka Promega.


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