Asiti ea Nucleic ea MTHFR ea Gene Polymorphic
Lebitso la sehlahisoa
Kiti ea ho Fumana Liphatsa tsa Lefutso la Liphatsa tsa Lefutso la HWTS-GE004-MTHFR (ARMS-PCR)
Thuto ea mafu a seoa
Folic acid ke vithamine e qhibilihang ka metsing e leng karolo ea bohlokoa ea 'mele litseleng tsa metabolism. Lilemong tsa morao tjena, lithuto tse ngata li tiisitse hore, phetoho ea gene ea enzyme e metabolizing ea folate MTHFR e tla lebisa khaellong ea folic acid 'meleng,' me tšenyo e tloaelehileng ea khaello ea folic acid ho batho ba baholo e ka baka khaello ea mali ea megaloblastic, tšenyo ea vascular endothelial, jj. Khaello ea folic acid ho basali ba bakhachane e ke ke ea khotsofatsa litlhoko tsa bona le tsa lesea le sa tsoa emoloa, e leng se ka bakang likoli tsa methapo ea kutlo, anencephaly, tsoalo ea lesea le shoeleng, le ho senyeheloa ke mpa. Maemo a serum folate a angoa ke li-polymorphisms tsa 5,10-methylenetetrahydrofolate reductase (MTHFR). Liphetoho tsa 677C>T le 1298A>C gene ea MTHFR li baka phetoho ea alanine ho valine le glutamic acid, ka ho latellana, e leng se fellang ka phokotso ea ts'ebetso ea MTHFR 'me ka lebaka leo phokotso ea ts'ebeliso ea folic acid.
Mokero
| FAM | MTHFR C677T |
| ROX | MTHFR A1298C |
| VIC(HEX) | Taolo ea ka hare |
Li-parameter tsa Tekheniki
| Polokelo | ≤-18℃ |
| Nako ea ho phela | Likhoeli tse 12 |
| Mofuta oa Sampole | Mali a sa tsoa bokelloa a EDTA a sa thibelang ho ruruha ha mali |
| CV | ≤5.0% |
| Ct | ≤38 |
| LoD | 1.0ng/μL |
| Lisebelisoa tse Sebetsang: | Mekhoa ea Li-biosystem tse Sebelisitsoeng 7500 Mekhoa ea PCR ea Nako ea Sebele Mekhoa ea PCR e Potlakileng ea Li-Biosystem tse 7500 tsa Nako ea 'Nete Mekhoa ea PCR ea SLAN ®-96P ea Nako ea 'Nete Mekhoa e 5 ea PCR ea QuantStudio™ ea Nako ea 'Nete Sistimi ea PCR ea LightCycler®480 ea Nako ea 'Nete Sistimi ea ho lemoha PCR ea LineGene 9600 Plus ea Nako ea 'Nete MA-6000 Nako ea Sebele ea Bongata ba Thermal Cycler Sistimi ea PCR ea BioRad CFX96 ea Nako ea 'Nete Sistimi ea PCR ea BioRad CFX Opus 96 ea Nako ea 'Nete |
Phallo ea Mosebetsi
Khetho ea 1
Li-reagent tse khothaletsoang tsa ho ntša: Kit ea DNA ea Genomic ea Macro le Micro-Test (HWTS-3014-32, HWTS-3014-48, HWTS-3014-96) le Macro le Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).
Khetho ea 2
Li-reagent tse khothaletsoang tsa ho ntša: Kit ea ho ntša DNA ea Genomic Genome ea Mali (YDP348, JCXB20210062) ea Tiangen Biotech(Beijing) Co.,Ltd. Kit ea ho ntša DNA ea Genome ea Mali (A1120) ea Promega.
