MTHFR Gene Polymorphic Nucleic Acid
Lebitso la sehlahisoa
HWTS-GE004-MTHFR Gene Polymorphic Nucleic Acid Detection Kit (ARMS-PCR)
Epidemiology
Folic acid ke vithamine e qhibilihang ka metsing eo e leng motsoako oa bohlokoa litseleng tsa 'mele oa metabolism.Lilemong tsa morao tjena, palo e kholo ea liphuputso e tiisitse hore, phetoho ea folate metabolizing enzyme gene MTHFR e tla lebisa khaellong ea folic acid 'meleng,' me tšenyo e tloaelehileng ea khaello ea folic acid ho batho ba baholo e ka baka megaloblastic anemia, vascular. tšenyo ea endothelial, joalo-joalo Ho haella ha folic acid ho basali ba bakhachane ha ba khone ho khotsofatsa litlhoko tsa bona le lesea le ka pōpelong, e leng se ka bakang bokooa ba methapo ea kutlo, anencephaly, pelehi, le ho senyeheloa ke mpa.Maemo a serum folate a angoa ke 5,10-methylenetetrahydrofolate reductase (MTHFR) polymorphisms.Liphetoho tsa 677C> T le 1298A> C ho liphatsa tsa lefutso tsa MTHFR li etsa hore ho fetoloa ha alanine ho valine le glutamic acid, ka ho latellana, ho fella ka ho fokotsa mosebetsi oa MTHFR 'me ka lebaka leo ho fokotsehe ho sebelisoa ha folic acid.
Channel
FAM | MTHFR C677T |
ROX | MTHFR A1298C |
VIC(HEX) | Taolo ya ka hare |
Tekheniki Parameters
Polokelo | ≤-18℃ |
Shelf-bophelo | Likhoeli tse 12 |
Mofuta oa Mohlala | Mali a sa tsoa bokelloa a EDTA a anticoagulated |
CV | ≤5.0% |
Ct | ≤38 |
LoD | 1.0ng/μL |
Lisebelisoa tse Sebetsang: | Applied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7500 Fast Real-Time PCR Systems SLAN ®-96P ea Nako ea 'Nete PCR Systems QuantStudio™ 5 ea Nako ea 'Nete ea PCR Systems Sesebelisoa sa PCR sa LightCycler®480 Real-Time LineGene 9600 Plus Nako ea 'Nete ea PCR ea ho Fumana Sistimi MA-6000 Real-Nako Quantitative Thermal Cycler Sistimi ea PCR ea Nako ea 'Nete ea BioRad CFX96 BioRad CFX Opus 96 Real-Nako PCR System |
Phallo ea Mosebetsi
Khetho ea 1
Li-reagents tse khothalelitsoeng: Macro & Micro-Test Genomic DNA Kit (HWTS-3014-32, HWTS-3014-48, HWTS-3014-96) le Macro & Micro-Test Automatic Nucleic Acid Extractor(HWTS-3006C, HWBTS-300) .
Khetho ea 2
Li-reagents tse khothalelitsoeng: Blood Genomic DNA Extraction Kit(YDP348, JCXB20210062) by Tiangen Biotech(Beijing) Co.,Ltd.Mali a Genome Extraction Kit(A1120) ka Promega.