Klebsiella Pneumoniae, Acinetobacter Baumannii le Pseudomonas Aeruginosa le Liphatsa tsa lefutso tsa ho hanyetsa lithethefatsi (KPC, NDM, OXA48 le IMP) Multiplex
Lebitso la sehlahisoa
HWTS-RT109 Klebsiella Pneumoniae, Acinetobacter Baumannii and Pseudomonas Aeruginosa and Drug Resistance Genes (KPC, NDM, OXA48 le IMP) Multiplex Detection Kit(Fluorescence PCR)
Setifikeiti
CE
Epidemiology
Klebsiella pneumoniae ke pathogen e tloaelehileng ea kliniki e nkang monyetla 'me ke e' ngoe ea libaktheria tsa bohlokoa tsa pathogenic tse bakang mafu a nosocomial. Ha ho hanyetsa ha 'mele ho fokotsehile, libaktheria li kena matšoafong ho tloha mokokotlong oa phefumoloho, ho baka tšoaetso likarolong tse ngata tsa' mele, 'me tšebeliso ea pele ea lithibela-mafu ke senotlolo sa ho phekola [1] .Sebaka se tloaelehileng sa tšoaetso ea Acinetobacter baumannii ke matšoafo, e leng kokoana-hloko ea bohlokoa bakeng sa Sepetlele se fumaneng pneumonia (HAP), haholo-holo Ventilator e amanang le pneumonia (VAP). Hangata e tsamaea le mafu a mang a baktheria le a fungal, a nang le litšobotsi tsa boemo bo phahameng ba ho kula le sekhahla se phahameng sa batho ba shoang.Pseudomonas aeruginosa ke likokoana-hloko tse sa tloaelehang tsa gram-negative ka mekhoa ea meriana, 'me ke likokoana-hloko tsa bohlokoa tse nkang monyetla bakeng sa tšoaetso e fumanoeng sepetlele, e nang le litšobotsi tsa bokolone bo bonolo, ho hanyetsa lithethefatsi tse ngata.
Tekheniki Parameters
| Polokelo | ≤-18℃ |
| Shelf-bophelo | Likhoeli tse 12 |
| Mofuta oa Mohlala | Sekhohlela |
| Ct | ≤36 |
| CV | ≤5.0% |
| LoD | 1000 likopi / mL |
| Tse khethehileng | a) Teko ea cross-reactivity e bontša hore kit ena ha e na reactivity ea sefapano le likokoana-hloko tse ling tsa phefumoloho, tse kang Streptococcus pneumoniae, Neisseria meningitidis, Staphylococcus aureus, Klebsiella oxytoca, Haemophilus influenzae, Acinetobacter jelly, Acinetobacter, Lefilaiphilaalytics, Epilalytics, Lebella oxytoca. Pseudomonas fluorescens, Candida albicans, Chlamydia pneumoniae, Respiratory Adenovirus, Enterococcus le lisampole tsa likhohlela ntle le liphofu, joalo-joalo. b) Bokhoni ba ho thibela tšitiso: Khetha mucin, minocycline, gentamicin, clindamycin, imipenem, cefoperazone, meropenem, ciprofloxacin hydrochloride, levofloxacin, clavulanic acid, le roxithromycin, joalo-joalo bakeng sa tlhahlobo ea tšitiso, 'me liphetho li bontša hore lintho tsa tšitiso tse boletsoeng ka holimo ha li kena-kenane le pneumonia e boletsoeng ka holimo. baumannii, Pseudomonas aeruginosa le liphatsa tsa lefutso tsa carbapenem KPC, NDM, OXA48 le IMP. |
| Lisebelisoa tse sebetsang | Applied Biosystems 7500 Real-Nako PCR Systems, Applied Biosystems 7500 Fast Real-Time PCR Systems, QuantStudio®Litsamaiso tse 5 tsa nako ea 'nete tsa PCR, LightCycler®Sistimi ea 480 ea Nako ea 'Nete ea PCR, LineGene 9600 Plus Nako ea 'Nete ea PCR Detection System (FQD-96A, theknoloji ea Bioer), MA-6000 Real-Nako Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.), Sistimi ea PCR ea Nako ea 'Nete ea BioRad CFX96, BioRad CFX Opus 96 Real-Nako PCR System. |
Phallo ea Mosebetsi
Macro & Micro-Test General DNA/RNA Kit (HWTS-3019) (e ka sebelisoang le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, (HWTS-3006B) by Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. IFU ea Kit.
