Human Leukocyte Antigen B27 Nucleic Acid
Lebitso la sehlahisoa
HWTS-GE011A-Human Leukocyte Antigen B27 Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Ankylosing spondylitis (AS) ke lefu le sa foleng le tsoelang pele le hlaselang haholo-holo le hlasela mokokotlo 'me le ka' na la kenyelletsa manonyeletso a sacroiliac le manonyeletso a potolohileng ho ea ka likhato tse sa tšoaneng.Ho senotsoe hore AS e bonts'a kopano e hlakileng ea malapa 'me e amana haufi-ufi le leukocyte antigen HLA-B27 ea motho.Ho batho, mefuta e fetang 70 ea li-subtypes tsa HLA-B27 li fumanoe le ho tsejoa, 'me ho tsona, HLA-B*2702, HLA-B*2704 le HLA-B*2705 ke mefuta e mengata e amanang le lefu lena.Chaena, Singapore, Japane le seterekeng sa Taiwan sa Chaena, mofuta o tloaelehileng haholo oa HLA-B27 ke HLA-B*2704, e nkang hoo e ka bang 54%, e lateloe ke HLA-B*2705, e etsang hoo e ka bang 41%.Setsi sena se ka lemoha DNA ka mefuta e meng ea HLA-B*2702, HLA-B*2704 le HLA-B*2705, empa ha e li khetholle ho tse ling.
Channel
| FAM | HLA-B27 |
| VIC/HEX | Taolo ea ka hare |
Tekheniki Parameters
| Polokelo | Mokelikeli: ≤-18℃ Lefifing |
| Shelf-bophelo | Mokelikeli: Likhoeli tse 18 |
| Mofuta oa Mohlala | disampole tse feletseng tsa madi |
| Ct | ≤40 |
| CV | ≤5.0% |
| LoD | 1ng/μL |
| Tse khethehileng
| Liphetho tsa liteko tse fumanoeng ke kit ena li ke ke tsa angoa ke hemoglobin (<800g/L), bilirubin (<700μmol/L), le lipids/triglycerides ea mali (<7mmol/L) maling. |
| Lisebelisoa tse sebetsang | Applied Biosystems 7500 Real-Time PCR Systems Lisebelisoa tsa Biosystems tse sebelisitsoeng StepOne Real-Time PCR Systems Sesebelisoa sa PCR sa LightCycler®480 Real-Time Sistimi ea Agilent-Stratagene Mx3000P Q-PCR |
