Tlhahlobo ea liphatsa tsa lefutso tsa kokoana-hloko ea Hepatitis B
Lebitso la sehlahisoa
Sete ea ho Fumana Li-Genotype tsa HWTS-HP002-Hepatitis B Virus (Fluorescent PCR)
Thuto ea mafu a seoa
Thuto ea mafu a seoa
Mokero
| MokeroLebitso | Sesebediswa sa ho Buffer sa Karabelo 1 | Reaction Buffer 2 |
| FAM | HBV-C | HBV-D |
| VIC/HEX | HBV-B | Taolo ea Ka Hare |
Li-parameter tsa Tekheniki
| Polokelo | ≤-18℃ Lefifing |
| Nako ea ho phela | Likhoeli tse 12 |
| Mofuta oa Sampole | Serum, Lero la mali |
| Ct | ≤38 |
| CV | ≤5.0％ |
| LoD | 1 × 102IU/mL |
| Ho hlaka | Ha ho na karabelo e ts'oanang le vaerase ea hepatitis C, cytomegalovirus ea motho, vaerase ea Epstein-Barr, vaerase ea immunodeficiency ea motho, vaerase ea hepatitis A, syphilis, vaerase ea herpes, vaerase ea influenza A, propionibacterium acnes (PA), jj. |
| Lisebelisoa tse Sebetsang | E ka bapisoa le lisebelisoa tse tloaelehileng tsa PCR tse khanyang tse rekisoang 'marakeng. Mekhoa ea PCR ea ABI 7500 ea Nako ea Sebele Mekhoa ea PCR e Potlakileng ea ABI 7500 ea Nako ea 'Nete Mekhoa ea PCR ea Nako ea Sebele ea SLAN-96P Mekhoa ea PCR ea QuantStudio®5 ea Nako ea 'Nete Mekhoa ea PCR ea LightCycler®480 ea Nako ea 'Nete Litsamaiso tsa ho lemoha PCR tsa LineGene 9600 Plus tsa Nako ea 'Nete MA-6000 Nako ea Sebele ea Bongata ba Thermal Cycler Sistimi ea PCR ea BioRad CFX96 ea Nako ea 'Nete Sistimi ea PCR ea BioRad CFX Opus 96 ea Nako ea 'Nete |
Reagent e kgothaletswang ya ho ntsha: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) e ka sebediswang le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) ke Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ho ntsha ho lokela ho etswa ho ya ka bukana ya ditaelo, bophahamo ba sampole bo ntshitsweng ke200μL, 'me bophahamo ba tharollo bo khothaletsoang ke80μL.
Reagent e kgothalletswang ho ntsha: Nucleic Acid Extraction kapa Purification Reagent (YDP315). Ho ntsha ho lokela ho etswa ho ya ka ditaelo. Bophahamo ba sampole e ntshitsweng ke 200µL, mme bophahamo ba elution e kgothalletswang ke 100µL.
