HCV Genotyping

Tlhaloso e Khutšoanyane:

Setsi sena se sebelisetsoa ho lemoha genotyping ea kokoana-hloko ea lefu la sebete la mofuta oa C (HCV) subtypes 1b, 2a, 3a, 3b le 6a ho serum ea kliniki ea serum / plasma ea kokoana-hloko ea hepatitis C (HCV).E thusa ho hlahloba le ho phekola bakuli ba HCV.

Re romelle lengolo-tsoibila

Lintlha tsa Sehlahisoa

Li-tag tsa Sehlahisoa

Lebitso la Sehlahiswa

HWTS-HP004-HCV Genotyping Detection Kit (Fluorescence PCR)

Epidemiology

Kokoana-hloko ea Hepatitis C (HCV) ke ea lelapa la flaviviridae, 'me genome ea eona ke khoele e le 'ngoe e ntle ea RNA, e fetohang habonolo.Kokoana-hloko e teng ho hepatocytes, serum leukocyte le plasma ea batho ba tšoaelitsoeng.Liphatsa tsa lefutso tsa HCV li kotsing ea ho fetoha 'me li ka aroloa bonyane li-genotypes tse 6 le mefuta e mengata e nyane.Mefuta e fapaneng ea HCV e sebelisa mefuta e fapaneng ea kalafo ea DAA le lithuto tsa kalafo.Ka hona, pele bakuli ba phekoloa ka phekolo ea DAA e thibelang likokoana-hloko, ho tlameha ho lemohuoe mofuta oa HCV, esita le ho bakuli ba nang le mofuta oa 1, hoa hlokahala ho khetholla hore na ke mofuta oa 1a kapa oa mofuta oa 1b.

Channel

FAM	Mofuta oa 1b, Mofuta oa 2a
ROX	Mofuta oa 6a, Mofuta oa 3a
VIC/HEX	Taolo ea ka hare, Mofuta oa 3b

Tekheniki Parameters

Polokelo	≤-18℃ Lefifing
Shelf-bophelo	Likhoeli tse 9
Mofuta oa Mohlala	Serum, Plasma
Ct	≤36
CV	≤5.0％
LoD	200 IU/mL
Tse khethehileng	Sebelisa sephutheloana sena ho bona lisampole tse ling tsa kokoana-hloko kapa baktheria tse kang: human cytomegalovirus, Epstein-Barr virus, human immunodeficiency virus, hepatitis B virus, hepatitis A virus, syphilis, human herpes virus type 6, herpes simplex virus type 1, simplex Herpes virus. mofuta oa 2, kokoana-hloko ea ntaramane A, Propionibacterium acnes, Staphylococcus aureus, Candida albicans, joalo-joalo Liphetho kaofela li mpe.
Lisebelisoa tse sebetsang	E ka tsamaisana le lisebelisoa tsa PCR tsa fluorescent tse fumanehang 'marakeng. ABI 7500 Real-Nako PCR Systems ABI 7500 Fast Real-Nako PCR Systems Litsamaiso tsa PCR tsa nako ea sebele tsa SLAN-96P QuantStudio®5 Nako ea 'Nete ea PCR Systems LightCycler®480 Real-Nako PCR Systems LineGene 9600 Plus Nako ea 'Nete ea PCR ea ho Khetholla Sistimi MA-6000 Real-Nako Quantitative Thermal Cycler Sistimi ea PCR ea Nako ea 'Nete ea BioRad CFX96 BioRad CFX Opus 96 Real-Nako PCR System