Tlhahlobo ea liphatsa tsa lefutso tsa HCV

Setšoantšo se Hlahisitsoeng sa HCV Genotyping

Tlhaloso e Khutšoanyane:

Sete ena e sebelisetsoa ho lemoha liphatsa tsa lefutso tsa kokoana-hloko ea hepatitis C (HCV) ea 1b, 2a, 3a, 3b le 6a lisampoleng tsa kliniki tsa serum/plasma tsa kokoana-hloko ea hepatitis C (HCV). E thusa ho hlahloba le ho phekola bakuli ba HCV.

Romella imeile ho rona

Qaqiso ea Sehlahisoa

Li-tag tsa Sehlahisoa

Lebitso la Sehlahisoa

Sete ea ho Lemoha Genotyping ea HWTS-HP004-HCV (Fluorescence PCR)

Thuto ea mafu a seoa

Vaerase ea Hepatitis C (HCV) ke ea lelapa la flaviviridae, 'me genome ea eona ke RNA e le' ngoe e ntle, e fetohang habonolo. Vaerase ena e teng ho li-hepatocyte, li-leukocyte tsa serum le plasma ea batho ba tšoaelitsoeng. Lijini tsa HCV li kotsing ea ho fetoha 'me li ka aroloa bonyane ka li-genotype tse 6 le mefuta e mengata. Li-genotype tse fapaneng tsa HCV li sebelisa mekhoa e fapaneng ea kalafo ea DAA le mekhoa ea kalafo. Ka hona, pele bakuli ba phekoloa ka phekolo ea antiviral ea DAA, genotype ea HCV e tlameha ho fumanoa, 'me esita le bakeng sa bakuli ba nang le mofuta oa 1, hoa hlokahala ho khetholla hore na ke mofuta oa 1a kapa mofuta oa 1b.

Mokero

FAM	Mofuta oa 1b, Mofuta oa 2a
ROX	Mofuta oa 6a, Mofuta oa 3a
VIC/HEX	Taolo ea Ka Hare, Mofuta oa 3b

Li-parameter tsa Tekheniki

Polokelo	≤-18℃ Lefifing
Nako ea ho phela	Likhoeli tse 9
Mofuta oa Sampole	Serum, Lero la mali
Ct	≤36
CV	≤5.0％
LoD	200 IU/mL
Ho hlaka	Sebelisa kit ena ho fumana disampole tse ding tsa vaerase kapa baktheria tse kang: human cytomegalovirus, Epstein-Barr virus, human immunodeficiency virus, hepatitis B virus, hepatitis A virus, syphilis, human herpes virus type 6, herpes simplex virus type 1, simplex Herpes virus type 2, influenza A virus, Propionibacterium acnes, Staphylococcus aureus, Candida albicans, jj. Diphetho kaofela ha di mpe.
Lisebelisoa tse Sebetsang	E ka bapisoa le lisebelisoa tse tloaelehileng tsa PCR tse khanyang tse rekisoang 'marakeng. Mekhoa ea PCR ea ABI 7500 ea Nako ea Sebele Mekhoa ea PCR e Potlakileng ea ABI 7500 ea Nako ea 'Nete Mekhoa ea PCR ea Nako ea Sebele ea SLAN-96P Mekhoa ea PCR ea QuantStudio®5 ea Nako ea 'Nete Mekhoa ea PCR ea LightCycler®480 ea Nako ea 'Nete Litsamaiso tsa ho lemoha PCR tsa LineGene 9600 Plus tsa Nako ea 'Nete MA-6000 Nako ea Sebele ea Bongata ba Thermal Cycler Sistimi ea PCR ea BioRad CFX96 ea Nako ea 'Nete Sistimi ea PCR ea BioRad CFX Opus 96 ea Nako ea 'Nete