Hantaan Virus Nucleic
Lebitso la sehlahisoa
HWTS-FE005 Hantaan Virus Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Hantavirus ke mofuta oa kokoana-hloko ea RNA e koahetsoeng, e arohaneng, e nang le mefuta e mebe. Hantavirus e arotsoe ka mefuta e ’meli: e ’ngoe e baka Hantavirus pulmonary syndrome (HPS), ’me e ’ngoe e baka Hantavirus hemorrhagic fever with renal syndrome (HFRS). Ea pele e atile haholo Europe le United States, 'me ea morao ke feberu ea mali e nang le lefu la renal le bakoang ke kokoana-hloko ea Hantaan, e atileng Chaena. Matšoao a mofuta oa hantavirus hantaan haholo-holo a iponahatsa e le feberu ea mali e nang le lefu la renal, le khetholloang ke feberu e matla, hypotension, mali a tsoang, oliguria kapa polyuria le ho senyeha ho hong ha renal. Ke pathogenic ho batho 'me e lokela ho lefshoa ka ho lekaneng.
Channel
| FAM | mofuta oa hantavirus hantaan |
| ROX | Taolo ea ka hare |
Tekheniki Parameters
| Polokelo | ≤-18℃ |
| Shelf-bophelo | Likhoeli tse 9 |
| Mofuta oa Mohlala | serum e ncha |
| Ct | ≤38 |
| CV | 5.0% |
| LoD | 500 Likopi / μL |
| Lisebelisoa tse sebetsang | Lisebelisoa tsa Biosystems 7500 tsa Nako ea 'Nete ea PCR Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®Litsamaiso tse 5 tsa nako ea 'nete tsa PCR Litsamaiso tsa PCR tsa nako ea sebele tsa SLAN-96P LightCycler®Sistimi ea 480 ea Nako ea 'Nete ea PCR LineGene 9600 Plus Nako ea 'Nete ea PCR ea ho Fumana Sistimi MA-6000 Real-Nako Quantitative Thermal Cycler Sistimi ea PCR ea Nako ea 'Nete ea BioRad CFX96 BioRad CFX Opus 96 Real-Nako PCR System |
Phallo ea Mosebetsi
Li-reagents tse khothalelitsoeng: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (e ka sebelisoang le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) ka Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Bophahamo ba sampole ea ho ntšoa ke 200μL. Molumo o khothaletsoang oa elution ke 80μL.
Li-reagents tse khothalelitsoeng: Nucleic Acid Extraction kapa Purification Kit (YDP315-R). Ho ntšoa ho lokela ho etsoa ho latela IFU. Bophahamo ba sampole ea ho ntšoa ke 140μL. Molumo o khothaletsoang oa elution ke 60μL.
