Vaerase ea Hantaan Nucleic
Lebitso la sehlahisoa
Kiti ea ho Fumana Asiti ea Nyutlelie ea HWTS-FE005 ea Virus ea Hantaan (Fluorescence PCR)
Thuto ea mafu a seoa
Hantavirus ke mofuta oa kokoana-hloko ea RNA e koahetsoeng, e arotsoeng ka likarolo, e nang le mela e mebe. Hantavirus e arotsoe ka mefuta e 'meli: e 'ngoe e baka Hantavirus pulmonary syndrome (HPS), 'me e 'ngoe e baka Hantavirus hemorrhagic fever with renal syndrome (HFRS). Ea pele e atile haholo Europe le United States, 'me ea bobeli ke feberu ea hemorrhagic with renal syndrome e bakoang ke kokoana-hloko ea Hantaan, e atileng Chaena. Matšoao a mofuta oa hantavirus hantaan a bonahala haholo-holo e le feberu ea hemorrhagic with renal syndrome, e khetholloang ka feberu e phahameng, hypotension, ho tsoa mali, oliguria kapa polyuria le ho fokola ho hong ha ts'ebetso ea liphio. E baka mafu ho batho 'me e lokela ho eloa hloko ka ho lekaneng.
Mokero
| FAM | mofuta oa hantavirus oa hantaan |
| ROX | Taolo ea Ka Hare |
Li-parameter tsa Tekheniki
| Polokelo | ≤-18℃ |
| Nako ea ho phela | Likhoeli tse 9 |
| Mofuta oa Sampole | serum e foreshe |
| Ct | ≤38 |
| CV | ＜5.0% |
| LoD | Likopi tse 500/μL |
| Lisebelisoa tse Sebetsang | Sistimi ea PCR ea Nako ea 'Nete e Sebelisitsoeng ea 7500 Mekhoa ea PCR e Potlakileng ea Li-Biosystem tse 7500 tsa Nako ea 'Nete QuantStudio®Mekhoa e 5 ea PCR ea Nako ea Sebele Mekhoa ea PCR ea Nako ea Sebele ea SLAN-96P Leseli la ho Tsamaisa Li-Cycler®Sistimi ea PCR ea Nako ea 'Nete ea 480 Sistimi ea ho lemoha PCR ea LineGene 9600 Plus ea Nako ea 'Nete MA-6000 Nako ea Sebele ea Bongata ba Thermal Cycler Sistimi ea PCR ea BioRad CFX96 ea Nako ea 'Nete Sistimi ea PCR ea BioRad CFX Opus 96 ea Nako ea 'Nete |
Phallo ea Mosebetsi
Li-reagent tse khothaletsoang tsa ho ntša: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (e ka sebelisoang le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) ke Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ho ntša ho lokela ho etsoa ho latela IFU. Bophahamo ba sampole ea ho ntša ke 200μL. Bophahamo ba elution bo khothaletsoang ke 80μL.
Li-reagent tse khothaletsoang tsa ho ntša: Nucleic Acid Extraction kapa Purification Kit (YDP315-R). Ho ntša ho lokela ho etsoa ho latela IFU. Bophahamo ba sampole ea ho ntša ke 140μL. Bophahamo ba elution bo khothaletsoang ke 60μL.
