Enterovirus Universal, EV71 le CoxA16
Lebitso la Sehlahiswa
HWTS-EV026B-Enterovirus Universal, EV71 le CoxA16 Nucleic Acid Detection Kit(Fluorescence PCR)
HWTS-EV020Y/Z- Enterovirus Universal e omisitsoeng ka leqhoa, EV71 le CoxA16 Nucleic Acid Detection Kit(Fluorescence PCR)
Setifikeiti
CE/MDA (HWTS-EV026)
Epidemiology
Hand-foot-mouth disease (HFMD) ke lefu le tšoaetsanoang le tšoaetsanoang le tloaelehileng ho bana. Hangata e hlaha ho bana ba ka tlase ho lilemo tse 5, 'me e ka baka herpes matsohong, maotong, molomong le likarolong tse ling,' me palo e nyenyane ea bana e ka baka mathata a kang myocarditis, pulmonary edema, aseptic meningoencephalitis, joalo-joalo Bana ka bomong ba nang le maloetse a tebileng ba senyeha ka potlako 'me ba kotsing ea ho shoa ha ba sa phekoloe hang-hang.
Hona joale, 108 serotypes ea enteroviruses li fumanoe, tse arotsoeng ka lihlopha tse 'nè: A, B, C le D. Enteroviruses tse bakang HFMD li fapane, empa enterovirus 71 (EV71) le coxsackievirus A16 (CoxA16) ke tsona tse tloaelehileng ka ho fetisisa 'me ho phaella ho HFMD, li ka baka mathata a tebileng a tsamaiso ea methapo e kang mencephalitis, complication e tebileng joalo ka mencephalitis, complication.
Channel
| FAM | Enterovirus |
| VIC (HEX) | CoxA16 |
| ROX | EV71 |
| CY5 | Taolo ya ka hare |
Tekheniki Parameters
| Polokelo | Mokelikeli: ≤-18℃ LefifingLyophilization: ≤30 ℃ |
| Shelf-bophelo | Mokelikeli: Likhoeli tse 9Lyophilization: likhoeli tse 12 |
| Mofuta oa Mohlala | Mohlala oa 'metso, Herpes fluid |
| Ct | ≤38 |
| CV | ≤5.0％ |
| LoD | 500Likopi / mL |
| Lisebelisoa tse sebetsang | E ka tsamaisana le lisebelisoa tsa PCR tsa fluorescent tse fumanehang 'marakeng.ABI 7500 Real-Nako PCR Systems ABI 7500 Fast Real-Nako PCR Systems Litsamaiso tsa PCR tsa nako ea sebele tsa SLAN-96P QuantStudio®5 Nako ea 'Nete ea PCR Systems LightCycler®480 Real-Nako PCR Systems LineGene 9600 Plus Nako ea 'Nete ea PCR ea ho Khetholla Sistimi MA-6000 Real-Nako Quantitative Thermal Cycler Sistimi ea PCR ea Nako ea 'Nete ea BioRad CFX96 BioRad CFX Opus 96 Real-Nako PCR System |
Kakaretso ea Tharollo ea PCR
Recommended reagent: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (e ka sebelisoang le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) by Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Bolumo ea sampole e ntšitsoeng ke 200μL, 'me molumo o khothaletsoang oa elution ke 80μL.
Sehlahisoa sa ho ntša se khothalelitsoeng: Macro & Micro-Test Sample Release Reagent (HWTS-3005-8). Ho ntšoa ho lokela ho etsoa ho latela bukana ea litaelo. Mehlala ea ho ntšoa ke li-oropharyngeal swabs kapa disampole tsa herpes fluid tsa bakuli tse bokelletsoeng sebakeng sa marang-rang. Eketsa li-swabs tse bokelletsoeng ka kotloloho ho Macro & Micro-Test Sample Release Reagent, vortex 'me u kopanye hantle, beha mocheso oa kamore metsotso e 5, u ntše, ebe u khelosa le ho kopanya hantle ho fumana RNA ea sampole ka' ngoe.
Sehlahisoa se khothalelitsoeng sa ho ntšoa: QIAamp Viral RNA Mini Kit (52904) ka QIAGEN kapa Nucleic Acid Extraction kapa Purification Reagent (YDP315-R). Ho ntšoa ho lokela ho etsoa ho latela tataiso e tiileng.
