Enterovirus Universal, EV71 le CoxA16
Lebitso la Sehlahisoa
Sete ea ho lemoha acid ea HWTS-EV026B-Enterovirus Universal, EV71 le CoxA16 Nucleic Acid (Fluorescence PCR)
HWTS-EV020Y/Z-Kit ea ho lemoha Enterovirus Universal, EV71 le CoxA16 Nucleic Acid e omisitsoeng ka leqhoa (Fluorescence PCR)
Setifikeiti
CE/MDA（HWTS-EV026）
Thuto ea mafu a seoa
Lefu la molomo la letsoho (HFMD) ke lefu le tšoaetsanoang le matla le tloaelehileng ho bana. Hangata le hlaha ho bana ba ka tlase ho lilemo tse 5, 'me le ka baka herpes matsohong, maotong, molomong le likarolong tse ling, 'me palo e nyane ea bana e ka baka mathata a kang myocarditis, pulmonary edema, aseptic meningoencephalitis, jj. Bana ka bomong ba nang le mafu a tebileng ba mpefala ka potlako 'me ba kotsing ea ho shoa haeba ba sa phekoloe kapele.
Hona jwale, ho fumanwe di-serotype tse 108 tsa di-enterovirus, tse arotsweng ka dihlopha tse nne: A, B, C le D. Di-enterovirus tse bakang HFMD di a fapana, empa enterovirus 71 (EV71) le coxsackievirus A16 (CoxA16) ke tsona tse atileng haholo mme ntle le HFMD, di ka baka mathata a tebileng a tsamaiso ya methapo ya kutlo jwalo ka meningitis, encephalitis, le ho holofala ha methapo ya kutlo.
Mokero
| FAM | Vaerase ea Enterovirus |
| VIC (HEX) | CoxA16 |
| ROX | EV71 |
| CY5 | Taolo ea ka hare |
Li-parameter tsa Tekheniki
| Polokelo | Metsi: ≤-18℃ LefifingLyophilization: ≤30℃ |
| Nako ea ho phela | Metsi: likhoeli tse 9Lyophilization: likhoeli tse 12 |
| Mofuta oa Sampole | Sampole ea sesepa sa 'metso, mokelikeli oa Herpes |
| Ct | ≤38 |
| CV | ≤5.0％ |
| LoD | Likopi tse 500/mL |
| Lisebelisoa tse Sebetsang | E ka bapisoa le lisebelisoa tse tloaelehileng tsa PCR tse khanyang tse rekisoang 'marakeng.Mekhoa ea PCR ea ABI 7500 ea Nako ea Sebele Mekhoa ea PCR e Potlakileng ea ABI 7500 ea Nako ea 'Nete Mekhoa ea PCR ea Nako ea Sebele ea SLAN-96P Mekhoa ea PCR ea QuantStudio®5 ea Nako ea 'Nete Mekhoa ea PCR ea LightCycler®480 ea Nako ea 'Nete Litsamaiso tsa ho lemoha PCR tsa LineGene 9600 Plus tsa Nako ea 'Nete MA-6000 Nako ea Sebele ea Bongata ba Thermal Cycler Sistimi ea PCR ea BioRad CFX96 ea Nako ea 'Nete Sistimi ea PCR ea BioRad CFX Opus 96 ea Nako ea 'Nete |
Tharollo ea PCR eohle
Reagent e kgothalletswang ho ntsha: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (e ka sebediswang le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) ke Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ho ntsha ho lokela ho etswa ho ya ka bukana ya ditaelo. Bophahamo ba sampole e ntshitsweng ke 200μL, mme bophahamo ba elution bo kgothaletswang ke 80μL.
Reagent e kgothalletswang ho ntsha: Reagent ya Macro & Micro-Test Sample Release Reagent (HWTS-3005-8). Ho ntsha ho lokela ho etswa ho ya ka bukana ya ditaelo. Disampole tsa ho ntsha ke diswab tsa oropharyngeal kapa disampole tsa metsi a herpes tsa bakudi tse bokelletsweng sebakeng seo. Kenya diswab tse bokelletsweng ka ho toba ho Reagent ya Macro & Micro-Test Sample Release, vortex mme o kopanye hantle, o di behe mochesong wa kamore metsotso e 5, o di ntshe ebe o di fetola mme o di kopanye hantle ho fumana RNA ya sampole ka nngwe.
Reagent e kgothaletswang ya ho ntsha: QIAamp Viral RNA Mini Kit (52904) ka QIAGEN kapa Nucleic Acid Extraction kapa Purification Reagent (YDP315-R). Ho ntsha ho lokela ho etswa ho ya ka ditaelo tse behilweng.
